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Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin.
Acta Crystallogr D Biol Crystallogr. 1999 Jan; 55(Pt 1):299-301.AC

Abstract

The prokaryotic ferritin gene of Campylobacter jejuni was overexpressed in Escherichia coli under control of the bacteriophage T7 promoter and the protein (Cj-FTN) purified. Preliminary crystallization experiments have been performed using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. Diffraction studies show the crystals belong to the I432 space group (a = 151.52 A). Structure solution by molecular replacement is in progress while crystal quality improvement is carried out.

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Authors+Show Affiliations

Clerté S
Unité de Biophysique Structurale (UPRES A 5471), Université de Bordeaux I, Avenue des Facultés, Bâtiment B8, 33405 Talence CEDEX, France.
Dautant A
No affiliation info available
Langlois d'Estaintot B
No affiliation info available
Gallois B
No affiliation info available
Mizunoe Y
No affiliation info available
Wai SN
No affiliation info available
Précigoux G
No affiliation info available

MeSH

Campylobacter jejuniCrystallizationCrystallography, X-RayEscherichia coliFerritinsGene ExpressionGenes, BacterialRecombinant Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10089431

Citation

Clerté, S, et al. "Expression, Purification, Crystallization and Preliminary X-ray Diffraction Results From Campylobacter Jejuni Ferritin." Acta Crystallographica. Section D, Biological Crystallography, vol. 55, no. Pt 1, 1999, pp. 299-301.
Clerté S, Dautant A, Langlois d'Estaintot B, et al. Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin. Acta Crystallogr D Biol Crystallogr. 1999;55(Pt 1):299-301.
Clerté, S., Dautant, A., Langlois d'Estaintot, B., Gallois, B., Mizunoe, Y., Wai, S. N., & Précigoux, G. (1999). Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin. Acta Crystallographica. Section D, Biological Crystallography, 55(Pt 1), 299-301.
Clerté S, et al. Expression, Purification, Crystallization and Preliminary X-ray Diffraction Results From Campylobacter Jejuni Ferritin. Acta Crystallogr D Biol Crystallogr. 1999;55(Pt 1):299-301. PubMed PMID: 10089431.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin. AU - Clerté,S, AU - Dautant,A, AU - Langlois d'Estaintot,B, AU - Gallois,B, AU - Mizunoe,Y, AU - Wai,S N, AU - Précigoux,G, Y1 - 1999/01/01/ PY - 1997/12/12/received PY - 1998/07/23/accepted PY - 1999/3/25/pubmed PY - 2000/6/23/medline PY - 1999/3/25/entrez SP - 299 EP - 301 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 55 IS - Pt 1 N2 - The prokaryotic ferritin gene of Campylobacter jejuni was overexpressed in Escherichia coli under control of the bacteriophage T7 promoter and the protein (Cj-FTN) purified. Preliminary crystallization experiments have been performed using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. Diffraction studies show the crystals belong to the I432 space group (a = 151.52 A). Structure solution by molecular replacement is in progress while crystal quality improvement is carried out. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/10089431/Expression_purification_crystallization_and_preliminary_X_ray_diffraction_results_from_Campylobacter_jejuni_ferritin_ L2 - http://scripts.iucr.org/cgi-bin/paper?S090744499801004X DB - PRIME DP - Unbound Medicine ER -