TY - JOUR T1 - Use of streptococcal protein G in obtaining crystals of an antibody Fab fragment in complex with a meningococcal antigen. AU - Derrick,J P, AU - Feavers,I, AU - Maiden,M C, Y1 - 1999/01/01/ PY - 1998/04/25/received PY - 1998/07/28/accepted PY - 1999/3/25/pubmed PY - 2000/6/23/medline PY - 1999/3/25/entrez SP - 314 EP - 6 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 55 IS - Pt 1 N2 - Crystals have been obtained of an antibody Fab fragment grown in the presence of a single domain from streptococcal protein G and a ten amino-acid peptide corresponding to the P1.7 serosubtype antigen from the human pathogen Neisseria meningitidis. Crystal trials using the Fab fragment and peptide antigen alone were unsuccessful, but the inclusion of a protein G domain provided an additional variable that generated suitable crystals. Crystals are in space group P21 with unit-cell parameters a = 43.60, b = 63.42, c = 89.63 A, beta = 98.58 degrees and a data set has been collected to 2.9 A resolution using synchrotron radiation. The inclusion of protein G is likely to be of general utility for the crystallization of Fab-antigen complexes. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/10089436/Use_of_streptococcal_protein_G_in_obtaining_crystals_of_an_antibody_Fab_fragment_in_complex_with_a_meningococcal_antigen_ L2 - http://scripts.iucr.org/cgi-bin/paper?S0907444998010269 DB - PRIME DP - Unbound Medicine ER -