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Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus.
Acta Crystallogr D Biol Crystallogr. 1999 Jan; 55(Pt 1):345-6.AC

Abstract

Crystallization of orotate phosphoribosyltransferase from a thermophilic organism, Thermus thermophilus, was achieved using the hanging-drop vapour-diffusion method coupled with a macroseeding starter. Small needle-like microcrystals were grown in a fresh protein solution in the presence of 2-methyl-2,4-pentanediol at 298 K or below. Although these normal temperature conditions caused stacking crystallization, an increase of temperature to 310 K permitted crystal growth. This was because of increased enzyme solubility at the higher temperature. The crystal was found to belong to the monoclinic space group P21with unit-cell parameters a = 44.4, b = 59.6, c = 67.8 A and beta = 98.3 degrees.

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Authors+Show Affiliations

Hamana H
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu Gunma 376, Japan.
Moriyama H
No affiliation info available
Shinozawa T
No affiliation info available
Tanaka N
No affiliation info available

MeSH

CrystallizationCrystallography, X-RayOrotate PhosphoribosyltransferaseTemperatureThermus thermophilus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10089446

Citation

Hamana, H, et al. "Medium Temperature, 310 K, Provides Single Crystals of Orotate Phosphoribosyltransferase From Thermus Thermophilus." Acta Crystallographica. Section D, Biological Crystallography, vol. 55, no. Pt 1, 1999, pp. 345-6.
Hamana H, Moriyama H, Shinozawa T, et al. Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus. Acta Crystallogr D Biol Crystallogr. 1999;55(Pt 1):345-6.
Hamana, H., Moriyama, H., Shinozawa, T., & Tanaka, N. (1999). Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus. Acta Crystallographica. Section D, Biological Crystallography, 55(Pt 1), 345-6.
Hamana H, et al. Medium Temperature, 310 K, Provides Single Crystals of Orotate Phosphoribosyltransferase From Thermus Thermophilus. Acta Crystallogr D Biol Crystallogr. 1999;55(Pt 1):345-6. PubMed PMID: 10089446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Medium temperature, 310 K, provides single crystals of orotate phosphoribosyltransferase from Thermus thermophilus. AU - Hamana,H, AU - Moriyama,H, AU - Shinozawa,T, AU - Tanaka,N, Y1 - 1999/01/01/ PY - 1997/12/03/received PY - 1998/06/09/accepted PY - 1999/3/25/pubmed PY - 2000/6/23/medline PY - 1999/3/25/entrez SP - 345 EP - 6 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 55 IS - Pt 1 N2 - Crystallization of orotate phosphoribosyltransferase from a thermophilic organism, Thermus thermophilus, was achieved using the hanging-drop vapour-diffusion method coupled with a macroseeding starter. Small needle-like microcrystals were grown in a fresh protein solution in the presence of 2-methyl-2,4-pentanediol at 298 K or below. Although these normal temperature conditions caused stacking crystallization, an increase of temperature to 310 K permitted crystal growth. This was because of increased enzyme solubility at the higher temperature. The crystal was found to belong to the monoclinic space group P21with unit-cell parameters a = 44.4, b = 59.6, c = 67.8 A and beta = 98.3 degrees. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/10089446/Medium_temperature_310_K_provides_single_crystals_of_orotate_phosphoribosyltransferase_from_Thermus_thermophilus_ L2 - http://scripts.iucr.org/cgi-bin/paper?S0907444998008130 DB - PRIME DP - Unbound Medicine ER -