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Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii.
Acta Crystallogr D Biol Crystallogr. 1999 Jan; 55(Pt 1):347-9.AC

Abstract

Recombinant uracil phosphoribosyltransferase (UPRT) enzyme of Toxoplasma gondii was expressed in Escherichia coli and purified from the cell-free extract by a combination of chromatographic steps. The recombinant protein was enzymatically active when tested in an in vitro UPRT assay. The purified protein was crystallized using the hanging-drop vapor-diffusion technique with ammonium phosphate as precipitant. The crystallized protein also exhibited UPRT activity. Crystals diffract to 2.4 A resolution and belong to space group P3121 or P3221 with unit-cell dimensions a = b = 119.9, c = 70.8 A and two molecules per asymmetric unit.

Authors+Show Affiliations

Center for Macromolecular Crystallography, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

10089447

Citation

Barchue, J, et al. "Expression, Purification, Crystallization and Preliminary X-ray Diffraction Analysis of Uracil Phosphoribosyltransferase of Toxoplasma Gondii." Acta Crystallographica. Section D, Biological Crystallography, vol. 55, no. Pt 1, 1999, pp. 347-9.
Barchue J, Symersky J, Narayana SV, et al. Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii. Acta Crystallogr D Biol Crystallogr. 1999;55(Pt 1):347-9.
Barchue, J., Symersky, J., Narayana, S. V., Moore, J. K., DeLucas, L. J., & Chattopadhyay, D. (1999). Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii. Acta Crystallographica. Section D, Biological Crystallography, 55(Pt 1), 347-9.
Barchue J, et al. Expression, Purification, Crystallization and Preliminary X-ray Diffraction Analysis of Uracil Phosphoribosyltransferase of Toxoplasma Gondii. Acta Crystallogr D Biol Crystallogr. 1999;55(Pt 1):347-9. PubMed PMID: 10089447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii. AU - Barchue,J, AU - Symersky,J, AU - Narayana,S V, AU - Moore,J K, AU - DeLucas,L J, AU - Chattopadhyay,D, Y1 - 1999/01/01/ PY - 1998/01/23/received PY - 1998/08/20/accepted PY - 1999/3/25/pubmed PY - 2000/6/23/medline PY - 1999/3/25/entrez SP - 347 EP - 9 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 55 IS - Pt 1 N2 - Recombinant uracil phosphoribosyltransferase (UPRT) enzyme of Toxoplasma gondii was expressed in Escherichia coli and purified from the cell-free extract by a combination of chromatographic steps. The recombinant protein was enzymatically active when tested in an in vitro UPRT assay. The purified protein was crystallized using the hanging-drop vapor-diffusion technique with ammonium phosphate as precipitant. The crystallized protein also exhibited UPRT activity. Crystals diffract to 2.4 A resolution and belong to space group P3121 or P3221 with unit-cell dimensions a = b = 119.9, c = 70.8 A and two molecules per asymmetric unit. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/10089447/Expression_purification_crystallization_and_preliminary_X_ray_diffraction_analysis_of_uracil_phosphoribosyltransferase_of_Toxoplasma_gondii_ L2 - http://scripts.iucr.org/cgi-bin/paper?S090744499800821X DB - PRIME DP - Unbound Medicine ER -