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Effect of acute and chronic exercise on plasma amino acids and prolactin concentrations and on [3H]ketanserin binding to serotonin2A receptors on human platelets.

Abstract

The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been shown to modulate various physiological and psychological functions such as fatigue. Altered regulation of the serotonergic system has been suggested to play a role in response to exercise stress. In the present study, the influence was investigated of acute endurance exercise and short-term increase in the amount of training on the concentrations of the 5-HT precursor tryptophan (TRP), of prolactin (PRL) and of branched-chain amino acids (BCAA) in the blood, as well as on the binding of [3H]ketanserin to the serotonin-2A (5-HT2A) receptors on platelets. Nine healthy endurance-trained men were tested the day before (I) and after (II) a 9-day training programme. Samples of venous blood were drawn after an overnight fast and following 5 h of cycling. Fasted and post-exercise plasma concentrations of free TRP, BCAA and free TRP:BCAA ratio did not differ between I and II. A significant decrease of plasma BCAA (P < 0.01) and significant augmentations of plasma free TRP, free TRP:BCAA ratio and PRL (P < 0.01) were found post-exercise. The increase in plasma PRL was smaller in II compared with I. Acute endurance exercise reduced the density of platelet 5-HT2A receptor [3H]ketanserin binding sites at I and II (P < 0.05). The basal density of the binding sites and the affinity of [3H]ketanserin for these binding sites were unaffected by an increase in the amount of training. The present results support the hypothesis that acute endurance exercise may increase 5-HT availability. This was reflected in the periphery by increased concentration of the 5-HT precursor free TRP, by increased plasma PRL concentration, and by a reduction of 5-HT2A receptors on platelets. It remains to be resolved whether these alterations in the periphery occur in parallel with an increase in the availability of 5-HT in the brain.

Authors+Show Affiliations

,

Institute of Sports Games, German Sport University, Cologne.

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Source

MeSH

Adult
Amino Acids
Amino Acids, Branched-Chain
Blood Platelets
Exercise
Humans
Ketanserin
Male
Osmolar Concentration
Physical Endurance
Prolactin
Receptors, Serotonin
Time Factors
Tritium
Tryptophan

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10090630

Citation

Strüder, H K., et al. "Effect of Acute and Chronic Exercise On Plasma Amino Acids and Prolactin Concentrations and On [3H]ketanserin Binding to serotonin2A Receptors On Human Platelets." European Journal of Applied Physiology and Occupational Physiology, vol. 79, no. 4, 1999, pp. 318-24.
Strüder HK, Hollmann W, Platen P, et al. Effect of acute and chronic exercise on plasma amino acids and prolactin concentrations and on [3H]ketanserin binding to serotonin2A receptors on human platelets. Eur J Appl Physiol Occup Physiol. 1999;79(4):318-24.
Strüder, H. K., Hollmann, W., Platen, P., Wöstmann, R., Weicker, H., & Molderings, G. J. (1999). Effect of acute and chronic exercise on plasma amino acids and prolactin concentrations and on [3H]ketanserin binding to serotonin2A receptors on human platelets. European Journal of Applied Physiology and Occupational Physiology, 79(4), pp. 318-24.
Strüder HK, et al. Effect of Acute and Chronic Exercise On Plasma Amino Acids and Prolactin Concentrations and On [3H]ketanserin Binding to serotonin2A Receptors On Human Platelets. Eur J Appl Physiol Occup Physiol. 1999;79(4):318-24. PubMed PMID: 10090630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of acute and chronic exercise on plasma amino acids and prolactin concentrations and on [3H]ketanserin binding to serotonin2A receptors on human platelets. AU - Strüder,H K, AU - Hollmann,W, AU - Platen,P, AU - Wöstmann,R, AU - Weicker,H, AU - Molderings,G J, PY - 1999/3/25/pubmed PY - 1999/3/25/medline PY - 1999/3/25/entrez SP - 318 EP - 24 JF - European journal of applied physiology and occupational physiology JO - Eur J Appl Physiol Occup Physiol VL - 79 IS - 4 N2 - The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been shown to modulate various physiological and psychological functions such as fatigue. Altered regulation of the serotonergic system has been suggested to play a role in response to exercise stress. In the present study, the influence was investigated of acute endurance exercise and short-term increase in the amount of training on the concentrations of the 5-HT precursor tryptophan (TRP), of prolactin (PRL) and of branched-chain amino acids (BCAA) in the blood, as well as on the binding of [3H]ketanserin to the serotonin-2A (5-HT2A) receptors on platelets. Nine healthy endurance-trained men were tested the day before (I) and after (II) a 9-day training programme. Samples of venous blood were drawn after an overnight fast and following 5 h of cycling. Fasted and post-exercise plasma concentrations of free TRP, BCAA and free TRP:BCAA ratio did not differ between I and II. A significant decrease of plasma BCAA (P < 0.01) and significant augmentations of plasma free TRP, free TRP:BCAA ratio and PRL (P < 0.01) were found post-exercise. The increase in plasma PRL was smaller in II compared with I. Acute endurance exercise reduced the density of platelet 5-HT2A receptor [3H]ketanserin binding sites at I and II (P < 0.05). The basal density of the binding sites and the affinity of [3H]ketanserin for these binding sites were unaffected by an increase in the amount of training. The present results support the hypothesis that acute endurance exercise may increase 5-HT availability. This was reflected in the periphery by increased concentration of the 5-HT precursor free TRP, by increased plasma PRL concentration, and by a reduction of 5-HT2A receptors on platelets. It remains to be resolved whether these alterations in the periphery occur in parallel with an increase in the availability of 5-HT in the brain. SN - 0301-5548 UR - https://www.unboundmedicine.com/medline/citation/10090630/Effect_of_acute_and_chronic_exercise_on_plasma_amino_acids_and_prolactin_concentrations_and_on_[3H]ketanserin_binding_to_serotonin2A_receptors_on_human_platelets_ L2 - https://medlineplus.gov/exerciseandphysicalfitness.html DB - PRIME DP - Unbound Medicine ER -