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Modulation of formyl peptide receptor expression by IL-10 in human monocytes and neutrophils.
J Immunol. 1999 Mar 15; 162(6):3590-5.JI

Abstract

IL-10, originally described as a cytokine synthesis inhibitory factor, is secreted by a number of cells of the immune system, including monocytes and T cells. Although IL-10 is being assigned as an immunosuppressive cytokine, our study showed that FMLP-R mRNA was rapidly up-regulated by exposure of monocytes to graded concentrations of this cytokine, with maximal (three- to fourfold) stimulation with 10 ng/ml. The effect was rapid, being observable as early as 1 h of treatment with IL-10, maximal between 2 and 4 h, and still evident after 24 h and was associated with an increase of receptor expression on the cell surface as assessed by flow cytometry analysis. Pretreatment of monocytes with actinomycin D completely abrogated the effect of IL-10, suggesting a transcriptional regulation. Moreover, IL-10-treated monocytes showed a significantly enhanced functional responsiveness to FMLP with enhanced (three- to fourfold) chemotaxis and augmented (twofold) intracellular calcium mobilization. In polymorphonuclear neutrophils (PMN), IL-10 also mediated a twofold augmentation of FMLP-R expression. In parallel experiments, we observed that IL-10 could differentially modulate other chemotactic receptors. Hence, we observed that IL-10 augmented two-to threefold platelet-activating factor receptor (PAF-R) expression, whereas it had no significant effect on the fifth component of complement (C5a) receptor (C5a-R) expression. Collectively, our results demonstrate that IL-10 may play an important role in inflammatory process through modulation of chemotactic receptor expression.

Authors+Show Affiliations

Department of Pediatrics, Faculty of Medicine, Université de Sherbrooke, Québec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10092818

Citation

Thivierge, M, et al. "Modulation of Formyl Peptide Receptor Expression By IL-10 in Human Monocytes and Neutrophils." Journal of Immunology (Baltimore, Md. : 1950), vol. 162, no. 6, 1999, pp. 3590-5.
Thivierge M, Parent JL, Stankova J, et al. Modulation of formyl peptide receptor expression by IL-10 in human monocytes and neutrophils. J Immunol. 1999;162(6):3590-5.
Thivierge, M., Parent, J. L., Stankova, J., & Rola-Pleszczynski, M. (1999). Modulation of formyl peptide receptor expression by IL-10 in human monocytes and neutrophils. Journal of Immunology (Baltimore, Md. : 1950), 162(6), 3590-5.
Thivierge M, et al. Modulation of Formyl Peptide Receptor Expression By IL-10 in Human Monocytes and Neutrophils. J Immunol. 1999 Mar 15;162(6):3590-5. PubMed PMID: 10092818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of formyl peptide receptor expression by IL-10 in human monocytes and neutrophils. AU - Thivierge,M, AU - Parent,J L, AU - Stankova,J, AU - Rola-Pleszczynski,M, PY - 1999/3/27/pubmed PY - 1999/3/27/medline PY - 1999/3/27/entrez SP - 3590 EP - 5 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 162 IS - 6 N2 - IL-10, originally described as a cytokine synthesis inhibitory factor, is secreted by a number of cells of the immune system, including monocytes and T cells. Although IL-10 is being assigned as an immunosuppressive cytokine, our study showed that FMLP-R mRNA was rapidly up-regulated by exposure of monocytes to graded concentrations of this cytokine, with maximal (three- to fourfold) stimulation with 10 ng/ml. The effect was rapid, being observable as early as 1 h of treatment with IL-10, maximal between 2 and 4 h, and still evident after 24 h and was associated with an increase of receptor expression on the cell surface as assessed by flow cytometry analysis. Pretreatment of monocytes with actinomycin D completely abrogated the effect of IL-10, suggesting a transcriptional regulation. Moreover, IL-10-treated monocytes showed a significantly enhanced functional responsiveness to FMLP with enhanced (three- to fourfold) chemotaxis and augmented (twofold) intracellular calcium mobilization. In polymorphonuclear neutrophils (PMN), IL-10 also mediated a twofold augmentation of FMLP-R expression. In parallel experiments, we observed that IL-10 could differentially modulate other chemotactic receptors. Hence, we observed that IL-10 augmented two-to threefold platelet-activating factor receptor (PAF-R) expression, whereas it had no significant effect on the fifth component of complement (C5a) receptor (C5a-R) expression. Collectively, our results demonstrate that IL-10 may play an important role in inflammatory process through modulation of chemotactic receptor expression. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/10092818/Modulation_of_formyl_peptide_receptor_expression_by_IL_10_in_human_monocytes_and_neutrophils_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=10092818 DB - PRIME DP - Unbound Medicine ER -