Tags

Type your tag names separated by a space and hit enter

Z/AP, a double reporter for cre-mediated recombination.
Dev Biol. 1999 Apr 15; 208(2):281-92.DB

Abstract

The Cre/loxP site-specific recombination system combined with embryonic stem cell-mediated technologies has greatly expanded our capability to address normal and disease development in mammals using genetic approaches. The success of this emerging technology hinges on the production of Cre-expressing transgenic lines that provide cell type-, tissue-, or developmental stage-specific recombination between loxP sites placed in the genome. Here we describe and characterize the production of a double-reporter mouse line that provides a convenient and reliable readout of Cre recombinase activity. Throughout all embryonic and adult stages, the transgenic animal expresses the lacZ reporter gene before Cre-mediated excision occurs. Cre excision, however, removes the lacZ gene, allowing expression of the second reporter, the human alkaline phosphatase gene. This double-reporter transgenic line is able to indicate the occurrence of Cre excision in an extremely widespread manner from early embryonic to adult lineages. It will be a valuable reagent for the increasing number of investigators taking advantage of the powerful tools provided by the Cre/loxP site-specific recombinase system.

Authors+Show Affiliations

Cancer Research Division, Sunnybrook Health Science Centre, 2075 Bayview Avenue, Toronto, Ontario, M4N 3M5, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10191045

Citation

Lobe, C G., et al. "Z/AP, a Double Reporter for Cre-mediated Recombination." Developmental Biology, vol. 208, no. 2, 1999, pp. 281-92.
Lobe CG, Koop KE, Kreppner W, et al. Z/AP, a double reporter for cre-mediated recombination. Dev Biol. 1999;208(2):281-92.
Lobe, C. G., Koop, K. E., Kreppner, W., Lomeli, H., Gertsenstein, M., & Nagy, A. (1999). Z/AP, a double reporter for cre-mediated recombination. Developmental Biology, 208(2), 281-92.
Lobe CG, et al. Z/AP, a Double Reporter for Cre-mediated Recombination. Dev Biol. 1999 Apr 15;208(2):281-92. PubMed PMID: 10191045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Z/AP, a double reporter for cre-mediated recombination. AU - Lobe,C G, AU - Koop,K E, AU - Kreppner,W, AU - Lomeli,H, AU - Gertsenstein,M, AU - Nagy,A, PY - 1999/4/7/pubmed PY - 1999/4/7/medline PY - 1999/4/7/entrez SP - 281 EP - 92 JF - Developmental biology JO - Dev Biol VL - 208 IS - 2 N2 - The Cre/loxP site-specific recombination system combined with embryonic stem cell-mediated technologies has greatly expanded our capability to address normal and disease development in mammals using genetic approaches. The success of this emerging technology hinges on the production of Cre-expressing transgenic lines that provide cell type-, tissue-, or developmental stage-specific recombination between loxP sites placed in the genome. Here we describe and characterize the production of a double-reporter mouse line that provides a convenient and reliable readout of Cre recombinase activity. Throughout all embryonic and adult stages, the transgenic animal expresses the lacZ reporter gene before Cre-mediated excision occurs. Cre excision, however, removes the lacZ gene, allowing expression of the second reporter, the human alkaline phosphatase gene. This double-reporter transgenic line is able to indicate the occurrence of Cre excision in an extremely widespread manner from early embryonic to adult lineages. It will be a valuable reagent for the increasing number of investigators taking advantage of the powerful tools provided by the Cre/loxP site-specific recombinase system. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/10191045/Z/AP_a_double_reporter_for_cre_mediated_recombination_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(99)99209-6 DB - PRIME DP - Unbound Medicine ER -