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Detection of Epstein-Barr virus (EBV) in hepatocellular carcinoma tissue: a novel EBV latency characterized by the absence of EBV-encoded small RNA expression.
Virology. 1999 Apr 10; 256(2):196-202.V

Abstract

In this study, we investigated the presence of Epstein-Barr virus (EBV) in liver tissue from 35 patients with hepatocellular carcinoma (HCC). EBV DNA was detected in 13 patients (37%) by Southern blot hybridization. In 10 of these patients, EBV DNA was present in tumor tissue only, whereas in the other 3, it was detected in both tumor and nontumor tissues. The quantity of EBV DNA detected was equivalent to 1-10 viral DNA molecules/100 cells. EBV-determined nuclear antigen was detected in 7-13% of the carcinoma cells in three tumor tissue samples that contained approximately one copy of the EBV genome/10 cells. A single terminal fragment of EBV DNA was identified in these tissues, suggesting that the EBV-infected cells in HCC represent clonal proliferation. Western blotting and reverse transcription-polymerase chain reaction analyses demonstrated that these three tumor tissue specimens were positive for EBV-determined nuclear antigen 1 and BamHI A transcripts but were negative for the other latent EBV products, including EBV-encoded small RNA. The results indicated that there is a high EBV load in HCC tissue and that all of the HCC tissue examined showed a novel pattern of EBV latency characterized by absence of EBV-encoded small RNA expression.

Authors+Show Affiliations

Cancer Institute, Hokkaido University School of Medicine, N15 W7, Kita-ku, Sapporo, 060-8638, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10191184

Citation

Sugawara, Y, et al. "Detection of Epstein-Barr Virus (EBV) in Hepatocellular Carcinoma Tissue: a Novel EBV Latency Characterized By the Absence of EBV-encoded Small RNA Expression." Virology, vol. 256, no. 2, 1999, pp. 196-202.
Sugawara Y, Mizugaki Y, Uchida T, et al. Detection of Epstein-Barr virus (EBV) in hepatocellular carcinoma tissue: a novel EBV latency characterized by the absence of EBV-encoded small RNA expression. Virology. 1999;256(2):196-202.
Sugawara, Y., Mizugaki, Y., Uchida, T., Torii, T., Imai, S., Makuuchi, M., & Takada, K. (1999). Detection of Epstein-Barr virus (EBV) in hepatocellular carcinoma tissue: a novel EBV latency characterized by the absence of EBV-encoded small RNA expression. Virology, 256(2), 196-202.
Sugawara Y, et al. Detection of Epstein-Barr Virus (EBV) in Hepatocellular Carcinoma Tissue: a Novel EBV Latency Characterized By the Absence of EBV-encoded Small RNA Expression. Virology. 1999 Apr 10;256(2):196-202. PubMed PMID: 10191184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Epstein-Barr virus (EBV) in hepatocellular carcinoma tissue: a novel EBV latency characterized by the absence of EBV-encoded small RNA expression. AU - Sugawara,Y, AU - Mizugaki,Y, AU - Uchida,T, AU - Torii,T, AU - Imai,S, AU - Makuuchi,M, AU - Takada,K, PY - 1999/4/7/pubmed PY - 1999/4/7/medline PY - 1999/4/7/entrez SP - 196 EP - 202 JF - Virology JO - Virology VL - 256 IS - 2 N2 - In this study, we investigated the presence of Epstein-Barr virus (EBV) in liver tissue from 35 patients with hepatocellular carcinoma (HCC). EBV DNA was detected in 13 patients (37%) by Southern blot hybridization. In 10 of these patients, EBV DNA was present in tumor tissue only, whereas in the other 3, it was detected in both tumor and nontumor tissues. The quantity of EBV DNA detected was equivalent to 1-10 viral DNA molecules/100 cells. EBV-determined nuclear antigen was detected in 7-13% of the carcinoma cells in three tumor tissue samples that contained approximately one copy of the EBV genome/10 cells. A single terminal fragment of EBV DNA was identified in these tissues, suggesting that the EBV-infected cells in HCC represent clonal proliferation. Western blotting and reverse transcription-polymerase chain reaction analyses demonstrated that these three tumor tissue specimens were positive for EBV-determined nuclear antigen 1 and BamHI A transcripts but were negative for the other latent EBV products, including EBV-encoded small RNA. The results indicated that there is a high EBV load in HCC tissue and that all of the HCC tissue examined showed a novel pattern of EBV latency characterized by absence of EBV-encoded small RNA expression. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/10191184/Detection_of_Epstein_Barr_virus__EBV__in_hepatocellular_carcinoma_tissue:_a_novel_EBV_latency_characterized_by_the_absence_of_EBV_encoded_small_RNA_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(99)99619-8 DB - PRIME DP - Unbound Medicine ER -