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Progression and regression of atherosclerosis in APOE3-Leiden transgenic mice: an immunohistochemical study.
Atherosclerosis. 1999 Mar; 143(1):15-25.A

Abstract

Apolipoprotein E3-Leiden (APOE3-Leiden) transgenic mice develop hyperlipidemia and are highly susceptible to diet-induced atherosclerosis. We have studied the progression and regression of atherosclerosis using immunohistochemistry. Female transgenic mice were fed a moderate fat diet to study atherosclerosis over a longer time period. Fatty streaks arose in the intima and consisted of lipid filled macrophages which differed in origin. All macrophages expressed the macrophage scavenger receptor while two thirds expressed sialoadhesin and were positive for an antibody recognizing marginal zone macrophages (MOMA-1). All macrophages were negative for the scavenger receptor MARCO and 50% were positive for CD4. Small fatty streaks contained CD-3 positive T-lymphocytes which were for more than 70% CD4-positive. ICAM-1 was positive both in atherosclerotic and control mice. In early plaques, fibrosis was observed on the luminal and medial site of the foam cells while smooth muscle cells were only observed in the fibrous cap. To study regression, we used a high fat, high cholesterol diet to rapidly induce atherosclerosis (14 weeks). The animals were then fed normal chow. Subsequently, atherosclerosis was assayed over time (4, 8, 16 weeks). Cholesterol levels dropped in 4 weeks to control levels. The animals did not show a significantly decrease in plaque size over time. but the percentage macrophages was significantly smaller in the animals after 4 weeks. In conclusion, the APOE3-Leiden mouse is a useful model to study the progression and regression of atherosclerosis.

Authors+Show Affiliations

Department of Cell Biology and Immunology, Vrije Universiteit, Amsterdam, The Netherlands. mg@ruly46.medfac.leidenuniv.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10208477

Citation

Gijbels, M J., et al. "Progression and Regression of Atherosclerosis in APOE3-Leiden Transgenic Mice: an Immunohistochemical Study." Atherosclerosis, vol. 143, no. 1, 1999, pp. 15-25.
Gijbels MJ, van der Cammen M, van der Laan LJ, et al. Progression and regression of atherosclerosis in APOE3-Leiden transgenic mice: an immunohistochemical study. Atherosclerosis. 1999;143(1):15-25.
Gijbels, M. J., van der Cammen, M., van der Laan, L. J., Emeis, J. J., Havekes, L. M., Hofker, M. H., & Kraal, G. (1999). Progression and regression of atherosclerosis in APOE3-Leiden transgenic mice: an immunohistochemical study. Atherosclerosis, 143(1), 15-25.
Gijbels MJ, et al. Progression and Regression of Atherosclerosis in APOE3-Leiden Transgenic Mice: an Immunohistochemical Study. Atherosclerosis. 1999;143(1):15-25. PubMed PMID: 10208477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Progression and regression of atherosclerosis in APOE3-Leiden transgenic mice: an immunohistochemical study. AU - Gijbels,M J, AU - van der Cammen,M, AU - van der Laan,L J, AU - Emeis,J J, AU - Havekes,L M, AU - Hofker,M H, AU - Kraal,G, PY - 1999/4/20/pubmed PY - 1999/4/20/medline PY - 1999/4/20/entrez SP - 15 EP - 25 JF - Atherosclerosis JO - Atherosclerosis VL - 143 IS - 1 N2 - Apolipoprotein E3-Leiden (APOE3-Leiden) transgenic mice develop hyperlipidemia and are highly susceptible to diet-induced atherosclerosis. We have studied the progression and regression of atherosclerosis using immunohistochemistry. Female transgenic mice were fed a moderate fat diet to study atherosclerosis over a longer time period. Fatty streaks arose in the intima and consisted of lipid filled macrophages which differed in origin. All macrophages expressed the macrophage scavenger receptor while two thirds expressed sialoadhesin and were positive for an antibody recognizing marginal zone macrophages (MOMA-1). All macrophages were negative for the scavenger receptor MARCO and 50% were positive for CD4. Small fatty streaks contained CD-3 positive T-lymphocytes which were for more than 70% CD4-positive. ICAM-1 was positive both in atherosclerotic and control mice. In early plaques, fibrosis was observed on the luminal and medial site of the foam cells while smooth muscle cells were only observed in the fibrous cap. To study regression, we used a high fat, high cholesterol diet to rapidly induce atherosclerosis (14 weeks). The animals were then fed normal chow. Subsequently, atherosclerosis was assayed over time (4, 8, 16 weeks). Cholesterol levels dropped in 4 weeks to control levels. The animals did not show a significantly decrease in plaque size over time. but the percentage macrophages was significantly smaller in the animals after 4 weeks. In conclusion, the APOE3-Leiden mouse is a useful model to study the progression and regression of atherosclerosis. SN - 0021-9150 UR - https://www.unboundmedicine.com/medline/citation/10208477/Progression_and_regression_of_atherosclerosis_in_APOE3_Leiden_transgenic_mice:_an_immunohistochemical_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9150(98)00263-9 DB - PRIME DP - Unbound Medicine ER -