Tags

Type your tag names separated by a space and hit enter

Molecular screening of potential HNPCC patients using a multiplex microsatellite PCR system.
Mol Cell Probes. 1999 Apr; 13(2):157-65.MC

Abstract

Microsatellite instability (MSI) is a molecular hallmark of the H ereditary N on- P olyposis C olorectal C ancer (HNPCC) syndrome occurring in about 80-90% of the tumours and also in sporadic tumours of different organs, albeit at lower frequency. Highly unstable colorectal tumours (MSI-H) have different histopathological features and tend to have a better prognosis compared to neoplasms without (MSS) or with low levels of microsatellite instability (MSI-L). Since MSI classification allows the identification of potential HNPCC patients and might represent a valuable diagnostic parameter an increasing demand for high-throughput microsatellite analysis will arise. Therefore, we have adapted five diagnostic microsatellites, m(odified) ACTC, mBAT26, mD5S107, mD5S406 and mD13S153, to allow coamplification. Using this multiplex polymerase chain reaction (PCR) system 29 colorectal tumour tissues with known MSI status could be unambiguously identified as MSI-H (13 cases) or MSI-L/MSS (16 cases). Highly unstable colorectal tumour detection frequency of individual markers reached 77% (mD5S406), 85% (mACTC), 85% (mD5S107), 92% (D13S153) and 100% (mBAT26) showing similar sensitivity but improved specificity as compared with a microsatellite reference panel. In a prospective analysis of 31 colorectal tumours, the multiplex PCR system identified five MSI-H cases. Multiplex MSI PCR is a time saving and cost-effective method not restricted to specific technical equipment and applicable to a variety of microsatellite-based genotyping approaches.

Authors+Show Affiliations

Division of Molecular Diagnostics and Therapy, University Hospital, Im Neuenheimer Feld 116, Heidelberg, D-69120, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10208807

Citation

Sutter, C, et al. "Molecular Screening of Potential HNPCC Patients Using a Multiplex Microsatellite PCR System." Molecular and Cellular Probes, vol. 13, no. 2, 1999, pp. 157-65.
Sutter C, Gebert J, Bischoff P, et al. Molecular screening of potential HNPCC patients using a multiplex microsatellite PCR system. Mol Cell Probes. 1999;13(2):157-65.
Sutter, C., Gebert, J., Bischoff, P., Herfarth, C., & von Knebel Doeberitz, M. (1999). Molecular screening of potential HNPCC patients using a multiplex microsatellite PCR system. Molecular and Cellular Probes, 13(2), 157-65.
Sutter C, et al. Molecular Screening of Potential HNPCC Patients Using a Multiplex Microsatellite PCR System. Mol Cell Probes. 1999;13(2):157-65. PubMed PMID: 10208807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular screening of potential HNPCC patients using a multiplex microsatellite PCR system. AU - Sutter,C, AU - Gebert,J, AU - Bischoff,P, AU - Herfarth,C, AU - von Knebel Doeberitz,M, PY - 1999/4/20/pubmed PY - 1999/4/20/medline PY - 1999/4/20/entrez SP - 157 EP - 65 JF - Molecular and cellular probes JO - Mol. Cell. Probes VL - 13 IS - 2 N2 - Microsatellite instability (MSI) is a molecular hallmark of the H ereditary N on- P olyposis C olorectal C ancer (HNPCC) syndrome occurring in about 80-90% of the tumours and also in sporadic tumours of different organs, albeit at lower frequency. Highly unstable colorectal tumours (MSI-H) have different histopathological features and tend to have a better prognosis compared to neoplasms without (MSS) or with low levels of microsatellite instability (MSI-L). Since MSI classification allows the identification of potential HNPCC patients and might represent a valuable diagnostic parameter an increasing demand for high-throughput microsatellite analysis will arise. Therefore, we have adapted five diagnostic microsatellites, m(odified) ACTC, mBAT26, mD5S107, mD5S406 and mD13S153, to allow coamplification. Using this multiplex polymerase chain reaction (PCR) system 29 colorectal tumour tissues with known MSI status could be unambiguously identified as MSI-H (13 cases) or MSI-L/MSS (16 cases). Highly unstable colorectal tumour detection frequency of individual markers reached 77% (mD5S406), 85% (mACTC), 85% (mD5S107), 92% (D13S153) and 100% (mBAT26) showing similar sensitivity but improved specificity as compared with a microsatellite reference panel. In a prospective analysis of 31 colorectal tumours, the multiplex PCR system identified five MSI-H cases. Multiplex MSI PCR is a time saving and cost-effective method not restricted to specific technical equipment and applicable to a variety of microsatellite-based genotyping approaches. SN - 0890-8508 UR - https://www.unboundmedicine.com/medline/citation/10208807/Molecular_screening_of_potential_HNPCC_patients_using_a_multiplex_microsatellite_PCR_system_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-8508(99)90231-1 DB - PRIME DP - Unbound Medicine ER -