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Determination of anabolic steroids by gas chromatography/negative-ion chemical ionization mass spectrometry and gas chromatography/negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization.
Rapid Commun Mass Spectrom. 1999; 13(5):376-80.RC

Abstract

A gas chromatography/mass spectrometry (GC/MS) method is described which uses negative ion chemical ionization (NCI) and tandem mass spectrometry (MS/MS) for the determination of eight anabolic steroids in human urine. Eight anabolic steroids were derivatized by heptafluorobutyric anhydride (HFBA), and were determined using GC/NCI-MS and GC/NCI-MS/MS. The linear correlation coefficients for calibration in NCI-MS/MS were in the range 0.9880-0.9988. This method of derivatization with HFBA for use with GC/NCI was useful in determinations of 19-norandrosterone, boldenone, 19-noretiocholanolone, 2-methylandrosterone, nandrolone, 1-methyleneandrosterone, 1-methylandrosterone, 4-dihydroboldenone and mesterolone. The detection limits of this procedure were 5-20 ppb at a signal-to-noise (S/N) ratio of 3.

Authors+Show Affiliations

Doping Control Centre, Korea Institute of Science and Technology, Cheongryang, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10209875

Citation

Choi, M H., et al. "Determination of Anabolic Steroids By Gas Chromatography/negative-ion Chemical Ionization Mass Spectrometry and Gas Chromatography/negative-ion Chemical Ionization Tandem Mass Spectrometry With Heptafluorobutyric Anhydride Derivatization." Rapid Communications in Mass Spectrometry : RCM, vol. 13, no. 5, 1999, pp. 376-80.
Choi MH, Chung BC, Lee W, et al. Determination of anabolic steroids by gas chromatography/negative-ion chemical ionization mass spectrometry and gas chromatography/negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization. Rapid Commun Mass Spectrom. 1999;13(5):376-80.
Choi, M. H., Chung, B. C., Lee, W., Lee, U. C., & Kim, Y. (1999). Determination of anabolic steroids by gas chromatography/negative-ion chemical ionization mass spectrometry and gas chromatography/negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization. Rapid Communications in Mass Spectrometry : RCM, 13(5), 376-80.
Choi MH, et al. Determination of Anabolic Steroids By Gas Chromatography/negative-ion Chemical Ionization Mass Spectrometry and Gas Chromatography/negative-ion Chemical Ionization Tandem Mass Spectrometry With Heptafluorobutyric Anhydride Derivatization. Rapid Commun Mass Spectrom. 1999;13(5):376-80. PubMed PMID: 10209875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of anabolic steroids by gas chromatography/negative-ion chemical ionization mass spectrometry and gas chromatography/negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization. AU - Choi,M H, AU - Chung,B C, AU - Lee,W, AU - Lee,U C, AU - Kim,Y, PY - 1999/4/21/pubmed PY - 2000/6/20/medline PY - 1999/4/21/entrez SP - 376 EP - 80 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 13 IS - 5 N2 - A gas chromatography/mass spectrometry (GC/MS) method is described which uses negative ion chemical ionization (NCI) and tandem mass spectrometry (MS/MS) for the determination of eight anabolic steroids in human urine. Eight anabolic steroids were derivatized by heptafluorobutyric anhydride (HFBA), and were determined using GC/NCI-MS and GC/NCI-MS/MS. The linear correlation coefficients for calibration in NCI-MS/MS were in the range 0.9880-0.9988. This method of derivatization with HFBA for use with GC/NCI was useful in determinations of 19-norandrosterone, boldenone, 19-noretiocholanolone, 2-methylandrosterone, nandrolone, 1-methyleneandrosterone, 1-methylandrosterone, 4-dihydroboldenone and mesterolone. The detection limits of this procedure were 5-20 ppb at a signal-to-noise (S/N) ratio of 3. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/10209875/Determination_of_anabolic_steroids_by_gas_chromatography/negative_ion_chemical_ionization_mass_spectrometry_and_gas_chromatography/negative_ion_chemical_ionization_tandem_mass_spectrometry_with_heptafluorobutyric_anhydride_derivatization_ L2 - https://doi.org/10.1002/(SICI)1097-0231(19990315)13:5<376::AID-RCM494>3.0.CO;2-# DB - PRIME DP - Unbound Medicine ER -