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Simultaneous determination of nitrite, nitrate and ascorbic acid in canned vegetable juices by reverse-phase ion-interaction HPLC.
Food Addit Contam. 1998 Oct; 15(7):753-8.FA

Abstract

A simple ion-interaction C18 reverse-phase high performance liquid chromatographic method was developed for simultaneous determination of nitrite, nitrate and ascorbic acid in canned vegetable juices. The method makes use of 0.010 M octylammonium orthophosphate as the ion interacting reagent and 20% (v/v) aqueous methanol as the mobile phase. The content of nitrite, nitrate (expressed as nitrite ion and nitrate ion, respectively) and ascorbic acid in commercial brands of canned tomato, carrot and mixed vegetable juices were surveyed. The quantitative results indicated that nitrite was very probably added as a preservative in the canned vegetable juices. The total amount of nitrite and nitrate ion per can in two commercial brands of vegetable juices was found to exceed the acceptable daily intake recommended by the 1996 Joint FAO/WHO Expert Committee on Food Additives for an average adult of 60 kg body weight.

Authors+Show Affiliations

Cheng CF
Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Kowloon, Hong Kong.
Tsang CW
No affiliation info available

MeSH

Ascorbic AcidBeveragesChromatography, High Pressure LiquidFood PreservationFood PreservativesHumansNitratesNitritesVegetables

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10211181

Citation

Cheng, C F., and C W. Tsang. "Simultaneous Determination of Nitrite, Nitrate and Ascorbic Acid in Canned Vegetable Juices By Reverse-phase Ion-interaction HPLC." Food Additives and Contaminants, vol. 15, no. 7, 1998, pp. 753-8.
Cheng CF, Tsang CW. Simultaneous determination of nitrite, nitrate and ascorbic acid in canned vegetable juices by reverse-phase ion-interaction HPLC. Food Addit Contam. 1998;15(7):753-8.
Cheng, C. F., & Tsang, C. W. (1998). Simultaneous determination of nitrite, nitrate and ascorbic acid in canned vegetable juices by reverse-phase ion-interaction HPLC. Food Additives and Contaminants, 15(7), 753-8.
Cheng CF, Tsang CW. Simultaneous Determination of Nitrite, Nitrate and Ascorbic Acid in Canned Vegetable Juices By Reverse-phase Ion-interaction HPLC. Food Addit Contam. 1998;15(7):753-8. PubMed PMID: 10211181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of nitrite, nitrate and ascorbic acid in canned vegetable juices by reverse-phase ion-interaction HPLC. AU - Cheng,C F, AU - Tsang,C W, PY - 1999/4/22/pubmed PY - 1999/4/22/medline PY - 1999/4/22/entrez SP - 753 EP - 8 JF - Food additives and contaminants JO - Food Addit Contam VL - 15 IS - 7 N2 - A simple ion-interaction C18 reverse-phase high performance liquid chromatographic method was developed for simultaneous determination of nitrite, nitrate and ascorbic acid in canned vegetable juices. The method makes use of 0.010 M octylammonium orthophosphate as the ion interacting reagent and 20% (v/v) aqueous methanol as the mobile phase. The content of nitrite, nitrate (expressed as nitrite ion and nitrate ion, respectively) and ascorbic acid in commercial brands of canned tomato, carrot and mixed vegetable juices were surveyed. The quantitative results indicated that nitrite was very probably added as a preservative in the canned vegetable juices. The total amount of nitrite and nitrate ion per can in two commercial brands of vegetable juices was found to exceed the acceptable daily intake recommended by the 1996 Joint FAO/WHO Expert Committee on Food Additives for an average adult of 60 kg body weight. SN - 0265-203X UR - https://www.unboundmedicine.com/medline/citation/10211181/Simultaneous_determination_of_nitrite_nitrate_and_ascorbic_acid_in_canned_vegetable_juices_by_reverse_phase_ion_interaction_HPLC_ L2 - https://medlineplus.gov/vitaminc.html DB - PRIME DP - Unbound Medicine ER -