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Effects of epinephrine on ouabain-sensitive, K(+) -dependent P-nitrophenylphosphatase activity in strial marginal cells of guinea pigs.
Ann Otol Rhinol Laryngol. 1999 Apr; 108(4):345-8.AO

Abstract

In strial marginal cells, Na+/K(+)-ATPase activity is abundant, and contributes to maintain the characteristic electrolyte composition of the cochlear endolymph. In the present study, to clarify the relationship between epinephrine and strial Na+/K(+)-ATPase activity, the ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K(+)-NPPase) activity of strial marginal cells was investigated with a cerium-based method in normal guinea pigs and guinea pigs treated with reserpine, epinephrine, and reserpine plus epinephrine. In our previous study, K(+)-NPPase activity had almost completely decreased 3 to 20 days after reserpine administration. In the present study, at 10 days after reserpinization and following repeated epinephrine treatment, enzyme activity was detectable. These results suggest that exogenous epinephrine was able to restore strial K(+)-NPPase activity in the reserpine-treated animals, and that epinephrine might increase strial Na+/K(+)-ATPase activity.

Authors+Show Affiliations

Department of Otolaryngology, Hyogo College of Medicine, Nishinomiya, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10214780

Citation

Kanoh, N. "Effects of Epinephrine On Ouabain-sensitive, K(+) -dependent P-nitrophenylphosphatase Activity in Strial Marginal Cells of Guinea Pigs." The Annals of Otology, Rhinology, and Laryngology, vol. 108, no. 4, 1999, pp. 345-8.
Kanoh N. Effects of epinephrine on ouabain-sensitive, K(+) -dependent P-nitrophenylphosphatase activity in strial marginal cells of guinea pigs. Ann Otol Rhinol Laryngol. 1999;108(4):345-8.
Kanoh, N. (1999). Effects of epinephrine on ouabain-sensitive, K(+) -dependent P-nitrophenylphosphatase activity in strial marginal cells of guinea pigs. The Annals of Otology, Rhinology, and Laryngology, 108(4), 345-8.
Kanoh N. Effects of Epinephrine On Ouabain-sensitive, K(+) -dependent P-nitrophenylphosphatase Activity in Strial Marginal Cells of Guinea Pigs. Ann Otol Rhinol Laryngol. 1999;108(4):345-8. PubMed PMID: 10214780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of epinephrine on ouabain-sensitive, K(+) -dependent P-nitrophenylphosphatase activity in strial marginal cells of guinea pigs. A1 - Kanoh,N, PY - 1999/4/24/pubmed PY - 1999/4/24/medline PY - 1999/4/24/entrez SP - 345 EP - 8 JF - The Annals of otology, rhinology, and laryngology JO - Ann Otol Rhinol Laryngol VL - 108 IS - 4 N2 - In strial marginal cells, Na+/K(+)-ATPase activity is abundant, and contributes to maintain the characteristic electrolyte composition of the cochlear endolymph. In the present study, to clarify the relationship between epinephrine and strial Na+/K(+)-ATPase activity, the ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K(+)-NPPase) activity of strial marginal cells was investigated with a cerium-based method in normal guinea pigs and guinea pigs treated with reserpine, epinephrine, and reserpine plus epinephrine. In our previous study, K(+)-NPPase activity had almost completely decreased 3 to 20 days after reserpine administration. In the present study, at 10 days after reserpinization and following repeated epinephrine treatment, enzyme activity was detectable. These results suggest that exogenous epinephrine was able to restore strial K(+)-NPPase activity in the reserpine-treated animals, and that epinephrine might increase strial Na+/K(+)-ATPase activity. SN - 0003-4894 UR - https://www.unboundmedicine.com/medline/citation/10214780/Effects_of_epinephrine_on_ouabain_sensitive_K_+___dependent_P_nitrophenylphosphatase_activity_in_strial_marginal_cells_of_guinea_pigs_ L2 - https://journals.sagepub.com/doi/10.1177/000348949910800405?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -