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Interrelationships of myofibrillar ATPase activity and metabolic properties of myosin heavy chain-based fibre types in rat skeletal muscle.
Histochem Cell Biol. 1999 Apr; 111(4):277-87.HC

Abstract

Myofibrillar ATPase (mATPase), succinate dehydrogenase (SDH) and alpha-glycerophosphate dehydrogenase (GPD) activities and cross-sectional area (CSA) were measured in fibres of rat medial gastrocnemius muscle using quantitative histochemistry. The same fibres were typed immunohistochemically using monoclonal antibodies specific to selected myosin heavy chain (MHC) isoforms. The values of mATPase, SDH, GPD and CSA formed a continuum, but significant differences in mean values were observed among fibre types of presumed homogeneous MHC content. Type I fibres had the lowest mATPase activity, followed in rank order by type IIA < type IID/X < type IIB. Type IIA fibres had the highest SDH activity, followed in rank order by type IID/X > type I > type IIB. The mean GPD activity was consistently ranked according to fibre type such that type IIB > type IID/X > type IIA > type I. Type IIA fibres were the smallest, type IIB fibres were the largest and types I and IID/X were of intermediate size. Significant interrelationships between mATPase, SDH, GPD and CSA values were found on a fibre-to-fibre basis. Consequently, discrimination of fibres according to their MHC content was possible on the basis of their mATPase, SDH, GPD and CSA profiles. These intrafibre interrelationships suggest that the MHC isoform is associated with phenotypic differences in contractile, metabolic and size properties of muscle fibre types.

Authors+Show Affiliations

Department of Comparative and Pathological Anatomy, Faculty of Veterinary Science, University of Cordoba, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10219627

Citation

Rivero, J L., et al. "Interrelationships of Myofibrillar ATPase Activity and Metabolic Properties of Myosin Heavy Chain-based Fibre Types in Rat Skeletal Muscle." Histochemistry and Cell Biology, vol. 111, no. 4, 1999, pp. 277-87.
Rivero JL, Talmadge RJ, Edgerton VR. Interrelationships of myofibrillar ATPase activity and metabolic properties of myosin heavy chain-based fibre types in rat skeletal muscle. Histochem Cell Biol. 1999;111(4):277-87.
Rivero, J. L., Talmadge, R. J., & Edgerton, V. R. (1999). Interrelationships of myofibrillar ATPase activity and metabolic properties of myosin heavy chain-based fibre types in rat skeletal muscle. Histochemistry and Cell Biology, 111(4), 277-87.
Rivero JL, Talmadge RJ, Edgerton VR. Interrelationships of Myofibrillar ATPase Activity and Metabolic Properties of Myosin Heavy Chain-based Fibre Types in Rat Skeletal Muscle. Histochem Cell Biol. 1999;111(4):277-87. PubMed PMID: 10219627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interrelationships of myofibrillar ATPase activity and metabolic properties of myosin heavy chain-based fibre types in rat skeletal muscle. AU - Rivero,J L, AU - Talmadge,R J, AU - Edgerton,V R, PY - 1999/4/29/pubmed PY - 1999/4/29/medline PY - 1999/4/29/entrez SP - 277 EP - 87 JF - Histochemistry and cell biology JO - Histochem Cell Biol VL - 111 IS - 4 N2 - Myofibrillar ATPase (mATPase), succinate dehydrogenase (SDH) and alpha-glycerophosphate dehydrogenase (GPD) activities and cross-sectional area (CSA) were measured in fibres of rat medial gastrocnemius muscle using quantitative histochemistry. The same fibres were typed immunohistochemically using monoclonal antibodies specific to selected myosin heavy chain (MHC) isoforms. The values of mATPase, SDH, GPD and CSA formed a continuum, but significant differences in mean values were observed among fibre types of presumed homogeneous MHC content. Type I fibres had the lowest mATPase activity, followed in rank order by type IIA < type IID/X < type IIB. Type IIA fibres had the highest SDH activity, followed in rank order by type IID/X > type I > type IIB. The mean GPD activity was consistently ranked according to fibre type such that type IIB > type IID/X > type IIA > type I. Type IIA fibres were the smallest, type IIB fibres were the largest and types I and IID/X were of intermediate size. Significant interrelationships between mATPase, SDH, GPD and CSA values were found on a fibre-to-fibre basis. Consequently, discrimination of fibres according to their MHC content was possible on the basis of their mATPase, SDH, GPD and CSA profiles. These intrafibre interrelationships suggest that the MHC isoform is associated with phenotypic differences in contractile, metabolic and size properties of muscle fibre types. SN - 0948-6143 UR - https://www.unboundmedicine.com/medline/citation/10219627/Interrelationships_of_myofibrillar_ATPase_activity_and_metabolic_properties_of_myosin_heavy_chain_based_fibre_types_in_rat_skeletal_muscle_ L2 - https://dx.doi.org/10.1007/s004180050358 DB - PRIME DP - Unbound Medicine ER -