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Leukocytes utilize both alpha4 and alpha5 integrins for intraislet infiltration in non-obese diabetic mice.
J Autoimmun. 1999 May; 12(3):167-76.JA

Abstract

The loss of insulin-producing cells during the development of type 1 diabetes is dependent on leukocyte infiltration of beta-islets in the pancreas. Injection of antibodies to integrins and their ligands has been shown to prevent the development of diabetes in non-obese diabetic (NOD) mice. However, little is known about the progression of infiltration by leukocytes after their homing and extravasation into the pancreas. In the present study, the effect of monoclonal antibodies (mAbs) to alpha4 and alpha5 integrins on leukocytes that had infiltrated the islets was characterized in NOD mice at 10 weeks of age when insulitis was in progress, or in mice with recent onset of diabetes. Injection of mAbs to either alpha4 or alpha5 integrins had little effect on the extent of leukocyte infiltration in 10-week-old or diabetic mice. In contrast, leukocyte infiltration was significantly reduced upon injection of mAbs to both alpha4 and alpha5 integrins. The reduction in leukocyte infiltration was due to decreases in the percentage of islets with intraislet infiltration. However, the observed effect of mAbs to alpha4 and alpha5 integrins was reversible, since intraislet infiltration resumed upon termination of antibody treatment. Results suggest that after homing to the pancreas, leukocytes utilize both alpha4 and alpha5 integrins for intraislet infiltration.

Authors+Show Affiliations

Department of Microbiology and Immunology, The John P. Robarts Research Institute, London, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10222026

Citation

Uniyal, S, et al. "Leukocytes Utilize Both Alpha4 and Alpha5 Integrins for Intraislet Infiltration in Non-obese Diabetic Mice." Journal of Autoimmunity, vol. 12, no. 3, 1999, pp. 167-76.
Uniyal S, Boeters L, Chakrabarti S, et al. Leukocytes utilize both alpha4 and alpha5 integrins for intraislet infiltration in non-obese diabetic mice. J Autoimmun. 1999;12(3):167-76.
Uniyal, S., Boeters, L., Chakrabarti, S., Singh, B., & Chan, B. M. (1999). Leukocytes utilize both alpha4 and alpha5 integrins for intraislet infiltration in non-obese diabetic mice. Journal of Autoimmunity, 12(3), 167-76.
Uniyal S, et al. Leukocytes Utilize Both Alpha4 and Alpha5 Integrins for Intraislet Infiltration in Non-obese Diabetic Mice. J Autoimmun. 1999;12(3):167-76. PubMed PMID: 10222026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leukocytes utilize both alpha4 and alpha5 integrins for intraislet infiltration in non-obese diabetic mice. AU - Uniyal,S, AU - Boeters,L, AU - Chakrabarti,S, AU - Singh,B, AU - Chan,B M, PY - 1999/5/1/pubmed PY - 1999/5/1/medline PY - 1999/5/1/entrez SP - 167 EP - 76 JF - Journal of autoimmunity JO - J Autoimmun VL - 12 IS - 3 N2 - The loss of insulin-producing cells during the development of type 1 diabetes is dependent on leukocyte infiltration of beta-islets in the pancreas. Injection of antibodies to integrins and their ligands has been shown to prevent the development of diabetes in non-obese diabetic (NOD) mice. However, little is known about the progression of infiltration by leukocytes after their homing and extravasation into the pancreas. In the present study, the effect of monoclonal antibodies (mAbs) to alpha4 and alpha5 integrins on leukocytes that had infiltrated the islets was characterized in NOD mice at 10 weeks of age when insulitis was in progress, or in mice with recent onset of diabetes. Injection of mAbs to either alpha4 or alpha5 integrins had little effect on the extent of leukocyte infiltration in 10-week-old or diabetic mice. In contrast, leukocyte infiltration was significantly reduced upon injection of mAbs to both alpha4 and alpha5 integrins. The reduction in leukocyte infiltration was due to decreases in the percentage of islets with intraislet infiltration. However, the observed effect of mAbs to alpha4 and alpha5 integrins was reversible, since intraislet infiltration resumed upon termination of antibody treatment. Results suggest that after homing to the pancreas, leukocytes utilize both alpha4 and alpha5 integrins for intraislet infiltration. SN - 0896-8411 UR - https://www.unboundmedicine.com/medline/citation/10222026/Leukocytes_utilize_both_alpha4_and_alpha5_integrins_for_intraislet_infiltration_in_non_obese_diabetic_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0896-8411(98)90268-2 DB - PRIME DP - Unbound Medicine ER -