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Detection of functional platelet-activating factor receptors on human tonsillar B lymphocytes.
J Immunol. 1999 May 01; 162(9):5498-505.JI

Abstract

Although platelet-activating factor (PAF) receptors have been found on B lymphoblastoid cell lines, the action of PAF on freshly isolated human B cells has not been clearly demonstrated. Using a sensitive semiquantitative reverse-transcriptase PCR, we have found PAF receptor mRNA expressed by tonsillar B lymphocytes, but little in T lymphocytes. Examination of Percoll-fractionated tonsillar B cells indicated that the low density (primarily germinal center cells) and medium density fractions had approximately twofold more PAF receptor mRNA relative to the high density fraction. PAF (10-7 M) stimulated increases in intracellular Ca2+ that were consistently higher in the low and medium density B lymphocytes compared with high density cells. The PAF receptor antagonist Web 2170 inhibited this. Addition of PAF, but not lyso- or enantio-PAF, induced four- to sixfold greater synthesis of IgM and IgG in low and medium density cells compared with unstimulated controls, but had little effect on Ig production by high density cells. To investigate how PAF may influence Ig synthesis, PAF-stimulated B cells were examined for production of the Th2-type cytokines IL-4 and IL-13. PAF induced IL-4 and IL-13 mRNA expression in 17% of CD20+ cells, and IL-4 was detected in cell supernatants after 48-72 h of culture. Together, these data strongly suggest that functional PAF receptors are expressed on B cells in tonsils.

Authors+Show Affiliations

Division of Allergy and Immunology, McGill University/Montreal Children's Hospital Research Institute, Quebec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10228030

Citation

Bastien, Y, et al. "Detection of Functional Platelet-activating Factor Receptors On Human Tonsillar B Lymphocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 162, no. 9, 1999, pp. 5498-505.
Bastien Y, Toledano BJ, Mehio N, et al. Detection of functional platelet-activating factor receptors on human tonsillar B lymphocytes. J Immunol. 1999;162(9):5498-505.
Bastien, Y., Toledano, B. J., Mehio, N., Cameron, L., Lamoukhaid, B., Renzi, P., Hamid, Q., & Mazer, B. D. (1999). Detection of functional platelet-activating factor receptors on human tonsillar B lymphocytes. Journal of Immunology (Baltimore, Md. : 1950), 162(9), 5498-505.
Bastien Y, et al. Detection of Functional Platelet-activating Factor Receptors On Human Tonsillar B Lymphocytes. J Immunol. 1999 May 1;162(9):5498-505. PubMed PMID: 10228030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of functional platelet-activating factor receptors on human tonsillar B lymphocytes. AU - Bastien,Y, AU - Toledano,B J, AU - Mehio,N, AU - Cameron,L, AU - Lamoukhaid,B, AU - Renzi,P, AU - Hamid,Q, AU - Mazer,B D, PY - 1999/5/5/pubmed PY - 1999/5/5/medline PY - 1999/5/5/entrez SP - 5498 EP - 505 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 162 IS - 9 N2 - Although platelet-activating factor (PAF) receptors have been found on B lymphoblastoid cell lines, the action of PAF on freshly isolated human B cells has not been clearly demonstrated. Using a sensitive semiquantitative reverse-transcriptase PCR, we have found PAF receptor mRNA expressed by tonsillar B lymphocytes, but little in T lymphocytes. Examination of Percoll-fractionated tonsillar B cells indicated that the low density (primarily germinal center cells) and medium density fractions had approximately twofold more PAF receptor mRNA relative to the high density fraction. PAF (10-7 M) stimulated increases in intracellular Ca2+ that were consistently higher in the low and medium density B lymphocytes compared with high density cells. The PAF receptor antagonist Web 2170 inhibited this. Addition of PAF, but not lyso- or enantio-PAF, induced four- to sixfold greater synthesis of IgM and IgG in low and medium density cells compared with unstimulated controls, but had little effect on Ig production by high density cells. To investigate how PAF may influence Ig synthesis, PAF-stimulated B cells were examined for production of the Th2-type cytokines IL-4 and IL-13. PAF induced IL-4 and IL-13 mRNA expression in 17% of CD20+ cells, and IL-4 was detected in cell supernatants after 48-72 h of culture. Together, these data strongly suggest that functional PAF receptors are expressed on B cells in tonsils. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/10228030/Detection_of_functional_platelet_activating_factor_receptors_on_human_tonsillar_B_lymphocytes_ DB - PRIME DP - Unbound Medicine ER -