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The importance of storage temperature to the quality of freeze-dried biological products.
Dev Biol Stand. 1976 Oct; 36:161-4.DB

Abstract

Several important practical steps are recognized in the process of freeze-drying of biologic material. The first of these is the selection of conditions optimal for the stability of the substance at the normal storage temperature, usually +5 degrees C. The second step involves the collection of material, storage at some choice freezing temperature (preferably around -20 degrees C) until use. The third step consists of the freeze-drying process itself, with preliminary freezing at another temperature, usually between -60 degrees and -80 degrees C. How these steps affect the quality of biologic products, proteins and polysaccharides has been the subject of study in our laboratories. The results have led us to the conclusion that molecular rearrangements occur at certain critical frozen temperatures which, in combination with time, leads to inactivation in some cases, stability in others. Such critical temperatures can be measured for other products and systems.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1030415

Citation

Tiru, M, and M B. Tiru. "The Importance of Storage Temperature to the Quality of Freeze-dried Biological Products." Developments in Biological Standardization, vol. 36, 1976, pp. 161-4.
Tiru M, Tiru MB. The importance of storage temperature to the quality of freeze-dried biological products. Dev Biol Stand. 1976;36:161-4.
Tiru, M., & Tiru, M. B. (1976). The importance of storage temperature to the quality of freeze-dried biological products. Developments in Biological Standardization, 36, 161-4.
Tiru M, Tiru MB. The Importance of Storage Temperature to the Quality of Freeze-dried Biological Products. Dev Biol Stand. 1976;36:161-4. PubMed PMID: 1030415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The importance of storage temperature to the quality of freeze-dried biological products. AU - Tiru,M, AU - Tiru,M B, PY - 1976/10/1/pubmed PY - 1976/10/1/medline PY - 1976/10/1/entrez SP - 161 EP - 4 JF - Developments in biological standardization JO - Dev. Biol. Stand. VL - 36 N2 - Several important practical steps are recognized in the process of freeze-drying of biologic material. The first of these is the selection of conditions optimal for the stability of the substance at the normal storage temperature, usually +5 degrees C. The second step involves the collection of material, storage at some choice freezing temperature (preferably around -20 degrees C) until use. The third step consists of the freeze-drying process itself, with preliminary freezing at another temperature, usually between -60 degrees and -80 degrees C. How these steps affect the quality of biologic products, proteins and polysaccharides has been the subject of study in our laboratories. The results have led us to the conclusion that molecular rearrangements occur at certain critical frozen temperatures which, in combination with time, leads to inactivation in some cases, stability in others. Such critical temperatures can be measured for other products and systems. SN - 0301-5149 UR - https://www.unboundmedicine.com/medline/citation/1030415/The_importance_of_storage_temperature_to_the_quality_of_freeze_dried_biological_products_ DB - PRIME DP - Unbound Medicine ER -