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Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures.
Nat Neurosci. 1999 May; 2(5):454-60.NN

Abstract

Synaptic strength can be altered by a variety of pre- or postsynaptic modifications. Here we test the hypothesis that long-term depression (LTD) involves a decrease in the number of glutamate receptors that are clustered at individual synapses in primary cultures of hippocampal neurons. Similar to a prominent form of LTD observed in hippocampal slices, LTD in hippocampal cultures required NMDA receptor activation and was accompanied by a decrease in the amplitude and frequency of miniature excitatory postsynaptic currents. Immunocytochemical analysis revealed that induction of LTD caused a concurrent decrease in the number of AMPA receptors clustered at synapses but had no effect on synaptic NMDA receptor clusters. These results suggest that a subtype-specific redistribution of synaptic glutamate receptors contributes to NMDA receptor-dependent LTD.

Authors+Show Affiliations

Carroll RC
Department of Psychiatry, University of California, San Francisco 94143, USA.
Lissin DV
No affiliation info available
von Zastrow M
No affiliation info available
Nicoll RA
No affiliation info available
Malenka RC
No affiliation info available

MeSH

AnimalsCells, CulturedExcitatory Postsynaptic PotentialsHippocampusImmunohistochemistryNeuronal PlasticityNeuronsPatch-Clamp TechniquesRatsRats, Sprague-DawleyReceptors, AMPAReceptors, GlutamateReceptors, N-Methyl-D-AspartateTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10321250

Citation

Carroll, R C., et al. "Rapid Redistribution of Glutamate Receptors Contributes to Long-term Depression in Hippocampal Cultures." Nature Neuroscience, vol. 2, no. 5, 1999, pp. 454-60.
Carroll RC, Lissin DV, von Zastrow M, et al. Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures. Nat Neurosci. 1999;2(5):454-60.
Carroll, R. C., Lissin, D. V., von Zastrow, M., Nicoll, R. A., & Malenka, R. C. (1999). Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures. Nature Neuroscience, 2(5), 454-60.
Carroll RC, et al. Rapid Redistribution of Glutamate Receptors Contributes to Long-term Depression in Hippocampal Cultures. Nat Neurosci. 1999;2(5):454-60. PubMed PMID: 10321250.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures. AU - Carroll,R C, AU - Lissin,D V, AU - von Zastrow,M, AU - Nicoll,R A, AU - Malenka,R C, PY - 1999/5/13/pubmed PY - 2001/3/23/medline PY - 1999/5/13/entrez SP - 454 EP - 60 JF - Nature neuroscience JO - Nat Neurosci VL - 2 IS - 5 N2 - Synaptic strength can be altered by a variety of pre- or postsynaptic modifications. Here we test the hypothesis that long-term depression (LTD) involves a decrease in the number of glutamate receptors that are clustered at individual synapses in primary cultures of hippocampal neurons. Similar to a prominent form of LTD observed in hippocampal slices, LTD in hippocampal cultures required NMDA receptor activation and was accompanied by a decrease in the amplitude and frequency of miniature excitatory postsynaptic currents. Immunocytochemical analysis revealed that induction of LTD caused a concurrent decrease in the number of AMPA receptors clustered at synapses but had no effect on synaptic NMDA receptor clusters. These results suggest that a subtype-specific redistribution of synaptic glutamate receptors contributes to NMDA receptor-dependent LTD. SN - 1097-6256 UR - https://www.unboundmedicine.com/medline/citation/10321250/Rapid_redistribution_of_glutamate_receptors_contributes_to_long_term_depression_in_hippocampal_cultures_ DB - PRIME DP - Unbound Medicine ER -