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Physostigmine blocked nicotinic acetylcholine receptors in rat sympathetic ganglion neurons.
Zhongguo Yao Li Xue Bao. 1997 Nov; 18(6):508-11.ZY

Abstract

AIM

To study the blocking mechanism of physostigmine (Phy) on nicotinic acetylcholine receptors (NAChR) in sympathetic neurons.

METHODS

The whole-cell patch-clamp technique was used to observe the effects of Phy on NAChR in the cultured sympathetic neurons from neonatal rat superior cervical ganglia (SCG).

RESULTS

Phy 5 -20 mumol.L-1 inhibited neuronal NAChR in a concentration-dependent manner and accelerated the desensitization of NAChR. Changing the membrane potential from -50 to -90 mV did not affect the blocking effect of Phy. Phy 200 mumol.L-1 did not induce any noticeable response in SCG neurons.

CONCLUSION

Phy blocked NAChR in the sympathetic ganglion neurons by interacting with the allosteric sites out of the binding sites and the open ionic channels of the receptors. Phy did not possess excitative effect on NAChR in SCG neurons.

Authors+Show Affiliations

Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10322906

Citation

Zheng, J Q., et al. "Physostigmine Blocked Nicotinic Acetylcholine Receptors in Rat Sympathetic Ganglion Neurons." Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica, vol. 18, no. 6, 1997, pp. 508-11.
Zheng JQ, He XP, Yang AZ, et al. Physostigmine blocked nicotinic acetylcholine receptors in rat sympathetic ganglion neurons. Zhongguo Yao Li Xue Bao. 1997;18(6):508-11.
Zheng, J. Q., He, X. P., Yang, A. Z., & Liu, C. G. (1997). Physostigmine blocked nicotinic acetylcholine receptors in rat sympathetic ganglion neurons. Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica, 18(6), 508-11.
Zheng JQ, et al. Physostigmine Blocked Nicotinic Acetylcholine Receptors in Rat Sympathetic Ganglion Neurons. Zhongguo Yao Li Xue Bao. 1997;18(6):508-11. PubMed PMID: 10322906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physostigmine blocked nicotinic acetylcholine receptors in rat sympathetic ganglion neurons. AU - Zheng,J Q, AU - He,X P, AU - Yang,A Z, AU - Liu,C G, PY - 1999/5/14/pubmed PY - 1999/5/14/medline PY - 1999/5/14/entrez SP - 508 EP - 11 JF - Zhongguo yao li xue bao = Acta pharmacologica Sinica JO - Zhongguo Yao Li Xue Bao VL - 18 IS - 6 N2 - AIM: To study the blocking mechanism of physostigmine (Phy) on nicotinic acetylcholine receptors (NAChR) in sympathetic neurons. METHODS: The whole-cell patch-clamp technique was used to observe the effects of Phy on NAChR in the cultured sympathetic neurons from neonatal rat superior cervical ganglia (SCG). RESULTS: Phy 5 -20 mumol.L-1 inhibited neuronal NAChR in a concentration-dependent manner and accelerated the desensitization of NAChR. Changing the membrane potential from -50 to -90 mV did not affect the blocking effect of Phy. Phy 200 mumol.L-1 did not induce any noticeable response in SCG neurons. CONCLUSION: Phy blocked NAChR in the sympathetic ganglion neurons by interacting with the allosteric sites out of the binding sites and the open ionic channels of the receptors. Phy did not possess excitative effect on NAChR in SCG neurons. SN - 0253-9756 UR - https://www.unboundmedicine.com/medline/citation/10322906/Physostigmine_blocked_nicotinic_acetylcholine_receptors_in_rat_sympathetic_ganglion_neurons_ L2 - http://www.chinaphar.com/1671-4083/18/508.pdf DB - PRIME DP - Unbound Medicine ER -