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Phylogenetic analysis of Ara+ and Ara- Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes.
J Clin Microbiol. 1999 Jun; 37(6):1906-12.JC

Abstract

A Burkholderia pseudomallei-like organism has recently been identified among some soil isolates of B. pseudomallei in an area with endemic melioidosis. This organism is almost identical to B. pseudomallei in terms of morphological and biochemical profiles, except that it differs in ability to assimilate L-arabinose. These Ara+ isolates are also less virulent than the Ara- isolates in animal models. In addition, clinical isolates of B. pseudomallei available to date are almost exclusively Ara-. These features suggested that these two organisms may belong to distinctive species. In this study, the 16S rRNA-encoding genes from five clinical (four Ara- and one Ara+) and nine soil isolates (five Ara- and four Ara+) of B. pseudomallei were sequenced. The nucleotide sequences and phylogenetic analysis indicated that the 16S rRNA-encoding gene of the Ara+ biotype was similar to but distinctively different from that of the Ara- soil isolates, which were identical to the classical clinical isolates of B. pseudomallei. The nucleotide sequence differences in the 16S rRNA-encoding gene appeared to be specific for the Ara+ or Ara- biotypes. The differences were, however, not sufficient for classification into a new species within the genus Burkholderia. A simple and rapid multiplex PCR procedure was developed to discriminate between Ara- and Ara+ B. pseudomallei isolates. This new method could also be incorporated into our previously reported nested PCR system for detecting B. pseudomallei in clinical specimens.

Authors+Show Affiliations

Laboratory of Cellular and Molecular Immunology, Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10325345

Citation

Dharakul, T, et al. "Phylogenetic Analysis of Ara+ and Ara- Burkholderia Pseudomallei Isolates and Development of a Multiplex PCR Procedure for Rapid Discrimination Between the Two Biotypes." Journal of Clinical Microbiology, vol. 37, no. 6, 1999, pp. 1906-12.
Dharakul T, Tassaneetrithep B, Trakulsomboon S, et al. Phylogenetic analysis of Ara+ and Ara- Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. J Clin Microbiol. 1999;37(6):1906-12.
Dharakul, T., Tassaneetrithep, B., Trakulsomboon, S., & Songsivilai, S. (1999). Phylogenetic analysis of Ara+ and Ara- Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. Journal of Clinical Microbiology, 37(6), 1906-12.
Dharakul T, et al. Phylogenetic Analysis of Ara+ and Ara- Burkholderia Pseudomallei Isolates and Development of a Multiplex PCR Procedure for Rapid Discrimination Between the Two Biotypes. J Clin Microbiol. 1999;37(6):1906-12. PubMed PMID: 10325345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phylogenetic analysis of Ara+ and Ara- Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. AU - Dharakul,T, AU - Tassaneetrithep,B, AU - Trakulsomboon,S, AU - Songsivilai,S, PY - 1999/5/15/pubmed PY - 1999/5/15/medline PY - 1999/5/15/entrez SP - 1906 EP - 12 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 37 IS - 6 N2 - A Burkholderia pseudomallei-like organism has recently been identified among some soil isolates of B. pseudomallei in an area with endemic melioidosis. This organism is almost identical to B. pseudomallei in terms of morphological and biochemical profiles, except that it differs in ability to assimilate L-arabinose. These Ara+ isolates are also less virulent than the Ara- isolates in animal models. In addition, clinical isolates of B. pseudomallei available to date are almost exclusively Ara-. These features suggested that these two organisms may belong to distinctive species. In this study, the 16S rRNA-encoding genes from five clinical (four Ara- and one Ara+) and nine soil isolates (five Ara- and four Ara+) of B. pseudomallei were sequenced. The nucleotide sequences and phylogenetic analysis indicated that the 16S rRNA-encoding gene of the Ara+ biotype was similar to but distinctively different from that of the Ara- soil isolates, which were identical to the classical clinical isolates of B. pseudomallei. The nucleotide sequence differences in the 16S rRNA-encoding gene appeared to be specific for the Ara+ or Ara- biotypes. The differences were, however, not sufficient for classification into a new species within the genus Burkholderia. A simple and rapid multiplex PCR procedure was developed to discriminate between Ara- and Ara+ B. pseudomallei isolates. This new method could also be incorporated into our previously reported nested PCR system for detecting B. pseudomallei in clinical specimens. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/10325345/Phylogenetic_analysis_of_Ara+_and_Ara__Burkholderia_pseudomallei_isolates_and_development_of_a_multiplex_PCR_procedure_for_rapid_discrimination_between_the_two_biotypes_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=10325345 DB - PRIME DP - Unbound Medicine ER -