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Costratification of a population of bipolar cells with the direction-selective circuitry of the rabbit retina.
J Comp Neurol. 1999 May 24; 408(1):97-106.JC

Abstract

We have stained a new population of bipolar cells in rabbit retina by using antibodies against the carbohydrate epitope, CD15. The CD15-positive bipolar cells comprise 6-8% of the total cone bipolar cells in peripheral retina. Their axonal and dendritic arbors are similar in size and range from 15 to 50 pm in diameter. The axonal arbors are narrowly stratified in sublamina b of the inner plexiform layer. Double label experiments using an antibody against the calcium binding protein, calbindin, or an antibody against protein kinase C, demonstrate that the CD15-positive bipolar cells are a separate population from the previously identified calbindin-positive cone bipolar cells and the rod bipolar cells. Labeling the processes of starburst amacrine cells with antibodies against choline acetyltransferase showed that the CD15-positive bipolar cells stratify within and slightly more distally to the processes of the ON-starburst amacrine cells. Confocal images of retinal wholemounts showed that the axons of the CD15-positive bipolar cells follow the pattern of the ON-starburst cells' processes. Axonal varicosities of the CD15-positive bipolar cells penetrate the bundles formed by the processes of the ON-starburst cells. This finding suggests that the CD15-positive bipolar cell provides input to the ON-starburst amacrine cells and/or the ON-plexus of the ON-OFF direction-selective ganglion cells.

Authors+Show Affiliations

Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115, USA. browns@helix.mgh.harvard.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10331582

Citation

Brown, S P., and R H. Masland. "Costratification of a Population of Bipolar Cells With the Direction-selective Circuitry of the Rabbit Retina." The Journal of Comparative Neurology, vol. 408, no. 1, 1999, pp. 97-106.
Brown SP, Masland RH. Costratification of a population of bipolar cells with the direction-selective circuitry of the rabbit retina. J Comp Neurol. 1999;408(1):97-106.
Brown, S. P., & Masland, R. H. (1999). Costratification of a population of bipolar cells with the direction-selective circuitry of the rabbit retina. The Journal of Comparative Neurology, 408(1), 97-106.
Brown SP, Masland RH. Costratification of a Population of Bipolar Cells With the Direction-selective Circuitry of the Rabbit Retina. J Comp Neurol. 1999 May 24;408(1):97-106. PubMed PMID: 10331582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Costratification of a population of bipolar cells with the direction-selective circuitry of the rabbit retina. AU - Brown,S P, AU - Masland,R H, PY - 1999/5/20/pubmed PY - 2000/6/20/medline PY - 1999/5/20/entrez SP - 97 EP - 106 JF - The Journal of comparative neurology JO - J Comp Neurol VL - 408 IS - 1 N2 - We have stained a new population of bipolar cells in rabbit retina by using antibodies against the carbohydrate epitope, CD15. The CD15-positive bipolar cells comprise 6-8% of the total cone bipolar cells in peripheral retina. Their axonal and dendritic arbors are similar in size and range from 15 to 50 pm in diameter. The axonal arbors are narrowly stratified in sublamina b of the inner plexiform layer. Double label experiments using an antibody against the calcium binding protein, calbindin, or an antibody against protein kinase C, demonstrate that the CD15-positive bipolar cells are a separate population from the previously identified calbindin-positive cone bipolar cells and the rod bipolar cells. Labeling the processes of starburst amacrine cells with antibodies against choline acetyltransferase showed that the CD15-positive bipolar cells stratify within and slightly more distally to the processes of the ON-starburst amacrine cells. Confocal images of retinal wholemounts showed that the axons of the CD15-positive bipolar cells follow the pattern of the ON-starburst cells' processes. Axonal varicosities of the CD15-positive bipolar cells penetrate the bundles formed by the processes of the ON-starburst cells. This finding suggests that the CD15-positive bipolar cell provides input to the ON-starburst amacrine cells and/or the ON-plexus of the ON-OFF direction-selective ganglion cells. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/10331582/Costratification_of_a_population_of_bipolar_cells_with_the_direction_selective_circuitry_of_the_rabbit_retina_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1999&volume=408&issue=1&spage=97 DB - PRIME DP - Unbound Medicine ER -