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Glucose uptake kinetics and transcription of HXT genes in chemostat cultures of Saccharomyces cerevisiae.
J Biol Chem. 1999 May 28; 274(22):15350-9.JB

Abstract

The kinetics of glucose transport and the transcription of all 20 members of the HXT hexose transporter gene family were studied in relation to the steady state in situ carbon metabolism of Saccharomyces cerevisiae CEN.PK113-7D grown in chemostat cultures. Cells were cultivated at a dilution rate of 0.10 h-1 under various nutrient-limited conditions (anaerobically glucose- or nitrogen-limited or aerobically glucose-, galactose-, fructose-, ethanol-, or nitrogen-limited), or at dilution rates ranging between 0.05 and 0.38 h-1 in aerobic glucose-limited cultures. Transcription of HXT1-HXT7 was correlated with the extracellular glucose concentration in the cultures. Transcription of GAL2, encoding the galactose transporter, was only detected in galactose-limited cultures. SNF3 and RGT2, two members of the HXT family that encode glucose sensors, were transcribed at low levels. HXT8-HXT17 transcripts were detected at very low levels. A consistent relationship was observed between the expression of individual HXT genes and the glucose transport kinetics determined from zero-trans influx of 14C-glucose during 5 s. This relationship was in broad agreement with the transport kinetics of Hxt1-Hxt7 and Gal2 deduced in previous studies on single-HXT strains. At lower dilution rates the glucose transport capacity estimated from zero-trans influx experiments and the residual glucose concentration exceeded the measured in situ glucose consumption rate. At high dilution rates, however, the estimated glucose transport capacity was too low to account for the in situ glucose consumption rate.

Authors+Show Affiliations

E. C. Slater Institute, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10336421

Citation

Diderich, J A., et al. "Glucose Uptake Kinetics and Transcription of HXT Genes in Chemostat Cultures of Saccharomyces Cerevisiae." The Journal of Biological Chemistry, vol. 274, no. 22, 1999, pp. 15350-9.
Diderich JA, Schepper M, van Hoek P, et al. Glucose uptake kinetics and transcription of HXT genes in chemostat cultures of Saccharomyces cerevisiae. J Biol Chem. 1999;274(22):15350-9.
Diderich, J. A., Schepper, M., van Hoek, P., Luttik, M. A., van Dijken, J. P., Pronk, J. T., Klaassen, P., Boelens, H. F., de Mattos, M. J., van Dam, K., & Kruckeberg, A. L. (1999). Glucose uptake kinetics and transcription of HXT genes in chemostat cultures of Saccharomyces cerevisiae. The Journal of Biological Chemistry, 274(22), 15350-9.
Diderich JA, et al. Glucose Uptake Kinetics and Transcription of HXT Genes in Chemostat Cultures of Saccharomyces Cerevisiae. J Biol Chem. 1999 May 28;274(22):15350-9. PubMed PMID: 10336421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucose uptake kinetics and transcription of HXT genes in chemostat cultures of Saccharomyces cerevisiae. AU - Diderich,J A, AU - Schepper,M, AU - van Hoek,P, AU - Luttik,M A, AU - van Dijken,J P, AU - Pronk,J T, AU - Klaassen,P, AU - Boelens,H F, AU - de Mattos,M J, AU - van Dam,K, AU - Kruckeberg,A L, PY - 1999/5/21/pubmed PY - 1999/5/21/medline PY - 1999/5/21/entrez SP - 15350 EP - 9 JF - The Journal of biological chemistry JO - J Biol Chem VL - 274 IS - 22 N2 - The kinetics of glucose transport and the transcription of all 20 members of the HXT hexose transporter gene family were studied in relation to the steady state in situ carbon metabolism of Saccharomyces cerevisiae CEN.PK113-7D grown in chemostat cultures. Cells were cultivated at a dilution rate of 0.10 h-1 under various nutrient-limited conditions (anaerobically glucose- or nitrogen-limited or aerobically glucose-, galactose-, fructose-, ethanol-, or nitrogen-limited), or at dilution rates ranging between 0.05 and 0.38 h-1 in aerobic glucose-limited cultures. Transcription of HXT1-HXT7 was correlated with the extracellular glucose concentration in the cultures. Transcription of GAL2, encoding the galactose transporter, was only detected in galactose-limited cultures. SNF3 and RGT2, two members of the HXT family that encode glucose sensors, were transcribed at low levels. HXT8-HXT17 transcripts were detected at very low levels. A consistent relationship was observed between the expression of individual HXT genes and the glucose transport kinetics determined from zero-trans influx of 14C-glucose during 5 s. This relationship was in broad agreement with the transport kinetics of Hxt1-Hxt7 and Gal2 deduced in previous studies on single-HXT strains. At lower dilution rates the glucose transport capacity estimated from zero-trans influx experiments and the residual glucose concentration exceeded the measured in situ glucose consumption rate. At high dilution rates, however, the estimated glucose transport capacity was too low to account for the in situ glucose consumption rate. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/10336421/Glucose_uptake_kinetics_and_transcription_of_HXT_genes_in_chemostat_cultures_of_Saccharomyces_cerevisiae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(19)73030-0 DB - PRIME DP - Unbound Medicine ER -