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Candida albicans mannan- and protein-induced humoral, cellular and cytokine responses in atopic dermatitis patients.
Clin Exp Allergy. 1999 Jun; 29(6):824-31.CE

Abstract

BACKGROUND

The cytokine observed most often in atopic dermatitis (AD) is IL-4, but a role for IL-5 and IFN-gamma in the late and delayed phase reactions has been suggested. In AD with head, neck and shoulder distribution, hypersensitivity to saprophytic yeasts is an important pathogenetic factor. The yeast allergens include both the mannan polysaccharides and the proteins. Mannans are major cross-reacting allergens likely to be involved in the pathogenesis of AD.

OBJECTIVE

To characterize the humoral, lymphoproliferative and cytokine (IL-2, 4, 5 and IFN-gamma) responses of peripheral blood mononuclear cells (PBMCs) induced by Candida albicans mannan and protein antigens in AD.

METHODS

Fifteen AD patients and seven healthy controls were included. Ficoll-isolated PBMCs were stimulated by PHA and laboratory-generated mannan and protein extracts of C. albicans. Lymphocyte proliferation was measured and cytokine production was studied by ELISA. The antigen-specific IgG and IgE antibodies were analysed by ELISA and nitrocellulose RAST.

RESULTS

In AD mannan (P < 0.005) and protein (P < 0.002), specific IgE levels were higher than in healthy controls. Both mannan and protein-specific lymphoproliferations (both: P < 0.02) were higher in AD than in healthy controls. Mannan, but not protein, induced long lasting IL-2 and IL-4 productions from 24 h lasting up to 66-96 h and IL-5 and IFN-gamma productions with elevated levels at 66 and 96 h. The mannan-induced IL-2 (P = 0.015) and IFN-gamma (P < 0.005) were increased in AD as compared with healthy controls. Significant correlations were seen between the protein-induced proliferation responses and both serum total IgE (r = 0.59, P < 0.01) and protein-specific IgE (r = 0.65, P < 0.005). The mannan-induced IL-2 responses correlated with the specific IgE (r = 0.62, P < 0.01) and proliferation (r = 0.51, P < 0.02) and S-IgE level (r = 0.71, P < 0. 002). Mannan-induced IL-4 and IFN-gamma productions also correlated (r = 0.43, P < 0.05).

CONCLUSIONS

C. albicans mannan induced elevated IL-2 and IFN-gamma responses in AD patients. The correlations of the cytokine responses with mannan-induced IgE and proliferation responses suggest that C. albicans mannan induced TH1 type cytokine responses are involved in AD.

Authors+Show Affiliations

Department of Pulmonary Diseases and Clinical Allergology, INSERM U454, Hôpital Arnaud de Villeneuve, Montpellier, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10336600

Citation

Savolainen, J, et al. "Candida Albicans Mannan- and Protein-induced Humoral, Cellular and Cytokine Responses in Atopic Dermatitis Patients." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 29, no. 6, 1999, pp. 824-31.
Savolainen J, Kosonen J, Lintu P, et al. Candida albicans mannan- and protein-induced humoral, cellular and cytokine responses in atopic dermatitis patients. Clin Exp Allergy. 1999;29(6):824-31.
Savolainen, J., Kosonen, J., Lintu, P., Viander, M., Pène, J., Kalimo, K., Terho, E. O., & Bousquet, J. (1999). Candida albicans mannan- and protein-induced humoral, cellular and cytokine responses in atopic dermatitis patients. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 29(6), 824-31.
Savolainen J, et al. Candida Albicans Mannan- and Protein-induced Humoral, Cellular and Cytokine Responses in Atopic Dermatitis Patients. Clin Exp Allergy. 1999;29(6):824-31. PubMed PMID: 10336600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Candida albicans mannan- and protein-induced humoral, cellular and cytokine responses in atopic dermatitis patients. AU - Savolainen,J, AU - Kosonen,J, AU - Lintu,P, AU - Viander,M, AU - Pène,J, AU - Kalimo,K, AU - Terho,E O, AU - Bousquet,J, PY - 1999/5/21/pubmed PY - 1999/5/21/medline PY - 1999/5/21/entrez SP - 824 EP - 31 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 29 IS - 6 N2 - BACKGROUND: The cytokine observed most often in atopic dermatitis (AD) is IL-4, but a role for IL-5 and IFN-gamma in the late and delayed phase reactions has been suggested. In AD with head, neck and shoulder distribution, hypersensitivity to saprophytic yeasts is an important pathogenetic factor. The yeast allergens include both the mannan polysaccharides and the proteins. Mannans are major cross-reacting allergens likely to be involved in the pathogenesis of AD. OBJECTIVE: To characterize the humoral, lymphoproliferative and cytokine (IL-2, 4, 5 and IFN-gamma) responses of peripheral blood mononuclear cells (PBMCs) induced by Candida albicans mannan and protein antigens in AD. METHODS: Fifteen AD patients and seven healthy controls were included. Ficoll-isolated PBMCs were stimulated by PHA and laboratory-generated mannan and protein extracts of C. albicans. Lymphocyte proliferation was measured and cytokine production was studied by ELISA. The antigen-specific IgG and IgE antibodies were analysed by ELISA and nitrocellulose RAST. RESULTS: In AD mannan (P < 0.005) and protein (P < 0.002), specific IgE levels were higher than in healthy controls. Both mannan and protein-specific lymphoproliferations (both: P < 0.02) were higher in AD than in healthy controls. Mannan, but not protein, induced long lasting IL-2 and IL-4 productions from 24 h lasting up to 66-96 h and IL-5 and IFN-gamma productions with elevated levels at 66 and 96 h. The mannan-induced IL-2 (P = 0.015) and IFN-gamma (P < 0.005) were increased in AD as compared with healthy controls. Significant correlations were seen between the protein-induced proliferation responses and both serum total IgE (r = 0.59, P < 0.01) and protein-specific IgE (r = 0.65, P < 0.005). The mannan-induced IL-2 responses correlated with the specific IgE (r = 0.62, P < 0.01) and proliferation (r = 0.51, P < 0.02) and S-IgE level (r = 0.71, P < 0. 002). Mannan-induced IL-4 and IFN-gamma productions also correlated (r = 0.43, P < 0.05). CONCLUSIONS: C. albicans mannan induced elevated IL-2 and IFN-gamma responses in AD patients. The correlations of the cytokine responses with mannan-induced IgE and proliferation responses suggest that C. albicans mannan induced TH1 type cytokine responses are involved in AD. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/10336600/Candida_albicans_mannan__and_protein_induced_humoral_cellular_and_cytokine_responses_in_atopic_dermatitis_patients_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0954-7894&amp;date=1999&amp;volume=29&amp;issue=6&amp;spage=824 DB - PRIME DP - Unbound Medicine ER -