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Flow cytometric DNA quantification in immunophenotyped cells as a sensitive method for determination of aneuploid multiple myeloma cells in peripheral blood stem cell harvests and bone marrow after therapy.
Bone Marrow Transplant. 1999 May; 23(9):895-900.BM

Abstract

The simultaneous measurement of DNA content and myeloma-related antigens (B-B4 or CD38) by flow cytometry is proposed as a method for the detection of aneuploid plasma cells in peripheral blood stem cell (PBSC) harvests and in bone marrow after therapy. In 30 patients with initially detected aneuploid myeloma cells we evaluated the bone marrow after therapy and in eight of these patients 23 PBSC harvests were analyzed. In 13 of 23 PBSC harvests aneuploid myeloma cells were detectable (range: 0.02-0.63%). In the bone marrow of the 30 patients aneuploid plasma cells were detectable in all samples after chemotherapy (range: 0.12-35.70%) and after autologous PBSC transplantation in two of three patients (0.21% and 0.03%). Furthermore the relationship between diploid and aneuploid plasma cells can be evaluated. In the PBSC harvests the percentage of aneuploid plasma cells is significantly lower than that of diploid plasma cells (P=0.006). In contrast, in bone marrow the aneuploid plasma cells are predominant in most patients even after therapy (24 of 30 patients; P=0.0055). In the case of initially detected aneuploid myeloma cells, a contamination with malignant cells can be estimated with a simple flow cytometric method in PBSC harvests and in bone marrow after therapy.

Authors+Show Affiliations

Medizinische Klinik I, Universitätsklinikum der TU, Dresden, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10338044

Citation

Nowak, R, et al. "Flow Cytometric DNA Quantification in Immunophenotyped Cells as a Sensitive Method for Determination of Aneuploid Multiple Myeloma Cells in Peripheral Blood Stem Cell Harvests and Bone Marrow After Therapy." Bone Marrow Transplantation, vol. 23, no. 9, 1999, pp. 895-900.
Nowak R, Oelschlägel U, Range U, et al. Flow cytometric DNA quantification in immunophenotyped cells as a sensitive method for determination of aneuploid multiple myeloma cells in peripheral blood stem cell harvests and bone marrow after therapy. Bone Marrow Transplant. 1999;23(9):895-900.
Nowak, R., Oelschlägel, U., Range, U., Bergmann, S., Bornhäuser, M., Hölig, C., Schuler, U., Krebs, U., Günther, H., Kroschinsky, F., & Ehninger, G. (1999). Flow cytometric DNA quantification in immunophenotyped cells as a sensitive method for determination of aneuploid multiple myeloma cells in peripheral blood stem cell harvests and bone marrow after therapy. Bone Marrow Transplantation, 23(9), 895-900.
Nowak R, et al. Flow Cytometric DNA Quantification in Immunophenotyped Cells as a Sensitive Method for Determination of Aneuploid Multiple Myeloma Cells in Peripheral Blood Stem Cell Harvests and Bone Marrow After Therapy. Bone Marrow Transplant. 1999;23(9):895-900. PubMed PMID: 10338044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometric DNA quantification in immunophenotyped cells as a sensitive method for determination of aneuploid multiple myeloma cells in peripheral blood stem cell harvests and bone marrow after therapy. AU - Nowak,R, AU - Oelschlägel,U, AU - Range,U, AU - Bergmann,S, AU - Bornhäuser,M, AU - Hölig,C, AU - Schuler,U, AU - Krebs,U, AU - Günther,H, AU - Kroschinsky,F, AU - Ehninger,G, PY - 1999/5/25/pubmed PY - 1999/5/25/medline PY - 1999/5/25/entrez SP - 895 EP - 900 JF - Bone marrow transplantation JO - Bone Marrow Transplant VL - 23 IS - 9 N2 - The simultaneous measurement of DNA content and myeloma-related antigens (B-B4 or CD38) by flow cytometry is proposed as a method for the detection of aneuploid plasma cells in peripheral blood stem cell (PBSC) harvests and in bone marrow after therapy. In 30 patients with initially detected aneuploid myeloma cells we evaluated the bone marrow after therapy and in eight of these patients 23 PBSC harvests were analyzed. In 13 of 23 PBSC harvests aneuploid myeloma cells were detectable (range: 0.02-0.63%). In the bone marrow of the 30 patients aneuploid plasma cells were detectable in all samples after chemotherapy (range: 0.12-35.70%) and after autologous PBSC transplantation in two of three patients (0.21% and 0.03%). Furthermore the relationship between diploid and aneuploid plasma cells can be evaluated. In the PBSC harvests the percentage of aneuploid plasma cells is significantly lower than that of diploid plasma cells (P=0.006). In contrast, in bone marrow the aneuploid plasma cells are predominant in most patients even after therapy (24 of 30 patients; P=0.0055). In the case of initially detected aneuploid myeloma cells, a contamination with malignant cells can be estimated with a simple flow cytometric method in PBSC harvests and in bone marrow after therapy. SN - 0268-3369 UR - https://www.unboundmedicine.com/medline/citation/10338044/Flow_cytometric_DNA_quantification_in_immunophenotyped_cells_as_a_sensitive_method_for_determination_of_aneuploid_multiple_myeloma_cells_in_peripheral_blood_stem_cell_harvests_and_bone_marrow_after_therapy_ L2 - https://doi.org/10.1038/sj.bmt.1701736 DB - PRIME DP - Unbound Medicine ER -