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Protection against development of otitis media induced by nontypeable Haemophilus influenzae by both active and passive immunization in a chinchilla model of virus-bacterium superinfection.
Infect Immun. 1999 Jun; 67(6):2746-62.II

Abstract

Three separate studies, two involving active-immunization regimens and one involving a passive-transfer protocol, were conducted to initially screen and ultimately more fully assess several nontypeable Haemophilus influenzae outer membrane proteins or their derivatives for their relative protective efficacy in chinchilla models of otitis media. Initial screening of these antigens (P5-fimbrin, lipoprotein D, and P6), delivered singly or in combination with either Freund's adjuvant or alum, indicated that augmented bacterial clearance from the nasopharynx, the middle ears, or both anatomical sites could be induced by parenteral immunization with P5-fimbrin combined with lipoprotein D, lipoprotein D alone, or the synthetic chimeric peptide LB1 (derived from P5-fimbrin), respectively. Data from a second study, wherein chinchillas were immunized with LB1 or lipoprotein D, each delivered with alum, again indicated that clearance of nontypeable H. influenzae could be augmented by immunization with either of these immunogens; however, when this adjuvant was used, both antibody titers in serum and efficacy were reduced. A third study was performed to investigate passive delivery of antisera directed against either LB1, lipoprotein D, nonacylated lipoprotein D, or a unique recombinant peptide designated LPD-LB1(f)2,1,3. The last three antiserum pools were generated by using the combined adjuvant of alum plus monophosphoryl lipid A. Passive transfer of sera specific for LB1 or LPD-LB1(f)2,1,3 to adenovirus-compromised chinchillas, prior to intranasal challenge with nontypeable H. influenzae, significantly reduced the severity of signs and incidence of otitis media which developed (P </= 0.001). Collectively, these data indicate the continued merit of further developing LB1 and LPD-LB1(f)2,1,3 as components of vaccines for otitis media.

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Authors+Show Affiliations

Bakaletz LO
Division of Otologic Research, Department of Otolaryngology, College of Medicine, The Ohio State University, Columbus, Ohio, USA. BakaletL@pediatrics.ohio-state.edu
Kennedy BJ
No affiliation info available
Novotny LA
No affiliation info available
Duquesne G
No affiliation info available
Cohen J
No affiliation info available
Lobet Y
No affiliation info available

MeSH

Amino Acid SequenceAnimalsApolipoproteinsApolipoproteins DBacterial Outer Membrane ProteinsChinchillaDisease Models, AnimalGene AmplificationHaemophilus InfectionsHaemophilus VaccinesHaemophilus influenzaeImmunization, PassiveImmunohistochemistryMolecular Sequence DataOtitis MediaOtitis Media with EffusionPolymerase Chain ReactionSuperinfectionTympanic MembraneVaccinationVaccines, SyntheticVirus Diseases

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10338477

Citation

Bakaletz, L O., et al. "Protection Against Development of Otitis Media Induced By Nontypeable Haemophilus Influenzae By Both Active and Passive Immunization in a Chinchilla Model of Virus-bacterium Superinfection." Infection and Immunity, vol. 67, no. 6, 1999, pp. 2746-62.
Bakaletz LO, Kennedy BJ, Novotny LA, et al. Protection against development of otitis media induced by nontypeable Haemophilus influenzae by both active and passive immunization in a chinchilla model of virus-bacterium superinfection. Infect Immun. 1999;67(6):2746-62.
Bakaletz, L. O., Kennedy, B. J., Novotny, L. A., Duquesne, G., Cohen, J., & Lobet, Y. (1999). Protection against development of otitis media induced by nontypeable Haemophilus influenzae by both active and passive immunization in a chinchilla model of virus-bacterium superinfection. Infection and Immunity, 67(6), 2746-62.
Bakaletz LO, et al. Protection Against Development of Otitis Media Induced By Nontypeable Haemophilus Influenzae By Both Active and Passive Immunization in a Chinchilla Model of Virus-bacterium Superinfection. Infect Immun. 1999;67(6):2746-62. PubMed PMID: 10338477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection against development of otitis media induced by nontypeable Haemophilus influenzae by both active and passive immunization in a chinchilla model of virus-bacterium superinfection. AU - Bakaletz,L O, AU - Kennedy,B J, AU - Novotny,L A, AU - Duquesne,G, AU - Cohen,J, AU - Lobet,Y, PY - 1999/5/25/pubmed PY - 1999/5/25/medline PY - 1999/5/25/entrez SP - 2746 EP - 62 JF - Infection and immunity JO - Infect Immun VL - 67 IS - 6 N2 - Three separate studies, two involving active-immunization regimens and one involving a passive-transfer protocol, were conducted to initially screen and ultimately more fully assess several nontypeable Haemophilus influenzae outer membrane proteins or their derivatives for their relative protective efficacy in chinchilla models of otitis media. Initial screening of these antigens (P5-fimbrin, lipoprotein D, and P6), delivered singly or in combination with either Freund's adjuvant or alum, indicated that augmented bacterial clearance from the nasopharynx, the middle ears, or both anatomical sites could be induced by parenteral immunization with P5-fimbrin combined with lipoprotein D, lipoprotein D alone, or the synthetic chimeric peptide LB1 (derived from P5-fimbrin), respectively. Data from a second study, wherein chinchillas were immunized with LB1 or lipoprotein D, each delivered with alum, again indicated that clearance of nontypeable H. influenzae could be augmented by immunization with either of these immunogens; however, when this adjuvant was used, both antibody titers in serum and efficacy were reduced. A third study was performed to investigate passive delivery of antisera directed against either LB1, lipoprotein D, nonacylated lipoprotein D, or a unique recombinant peptide designated LPD-LB1(f)2,1,3. The last three antiserum pools were generated by using the combined adjuvant of alum plus monophosphoryl lipid A. Passive transfer of sera specific for LB1 or LPD-LB1(f)2,1,3 to adenovirus-compromised chinchillas, prior to intranasal challenge with nontypeable H. influenzae, significantly reduced the severity of signs and incidence of otitis media which developed (P </= 0.001). Collectively, these data indicate the continued merit of further developing LB1 and LPD-LB1(f)2,1,3 as components of vaccines for otitis media. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/10338477/Protection_against_development_of_otitis_media_induced_by_nontypeable_Haemophilus_influenzae_by_both_active_and_passive_immunization_in_a_chinchilla_model_of_virus_bacterium_superinfection_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&amp;pmid=10338477 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓59 ↑72]

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