Tags

Type your tag names separated by a space and hit enter

Plasma concentrations of carotenoids in healthy volunteers after intervention with carotenoid-rich foods.

Abstract

AIM OF THE STUDY

The present study was conducted to investigate changes in the plasma concentration of carotenoids and carotenoid oxidation products, vitamin A, alpha- and gamma-tocopherol, and ubiquinone-10 during a dietary intervention trial with 23 male healthy volunteers.

METHOD

A two week carotenoid depletion period was followed by a daily consumption of 330 mL tomato juice (40 mg lycopene), then by 330 mL carrot juice (15.7 mg alpha-carotene and 22.3 mg beta-carotene), and then by a 10 g spinach powder preparation (11.3 mg lutein and 3.1 mg beta-carotene) served with main meals for two weeks, respectively. Blood samples were collected in the morning after an overnight fasting and carotenoids, vitamin A, tocopherols, and ubichinone were analyzed by reversed-phase HPLC.

RESULTS

During the tomato juice intervention, plasma concentrations of trans- and cis-lycopene increased 3-fold compared to the depletion period. Lycopene oxidation products could be demonstrated in plasma and were significantly elevated compared to control (p < 0.001). After two weeks of carrot juice consumption, alpha-carotene and beta-carotene concentrations increased 8.6- and 3.2-fold, respectively. Finally, during the spinach consumption period the lutein concentration increased 2-fold, while the beta-carotene concentrations were still elevated 2-fold.

CONCLUSIONS

The moderate change in dietary habits, e.g., the consumption of 330 mL of carotenoid-rich vegetable juices caused significant changes in the plasma carotenoid concentrations, indicating a high bioavailability of carotenoids from these processed vegetable products. The changes in plasma carotenoid concentrations reflected the carotenoid composition of the consumed foods. However, particularly during the tomato juice intervention period the occurrence of lycopene oxidation products and cis-lycopene isomers in plasma was eminent. The formation may be due to antioxidant reactions of lycopene in the organism.

Authors+Show Affiliations

,

Federal Research Center for Nutrition, Institute of Nutritional Physiology, Karlsruhe, Germany.

, ,

Source

European journal of nutrition 38:1 1999 Feb pg 35-44

MeSH

Adult
Antioxidants
Carotenoids
Cholesterol
Diet
Fruit
Humans
Lycopene
Male
Reference Values
Vegetables
beta Carotene

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10338686

Citation

Müller, H, et al. "Plasma Concentrations of Carotenoids in Healthy Volunteers After Intervention With Carotenoid-rich Foods." European Journal of Nutrition, vol. 38, no. 1, 1999, pp. 35-44.
Müller H, Bub A, Watzl B, et al. Plasma concentrations of carotenoids in healthy volunteers after intervention with carotenoid-rich foods. Eur J Nutr. 1999;38(1):35-44.
Müller, H., Bub, A., Watzl, B., & Rechkemmer, G. (1999). Plasma concentrations of carotenoids in healthy volunteers after intervention with carotenoid-rich foods. European Journal of Nutrition, 38(1), pp. 35-44.
Müller H, et al. Plasma Concentrations of Carotenoids in Healthy Volunteers After Intervention With Carotenoid-rich Foods. Eur J Nutr. 1999;38(1):35-44. PubMed PMID: 10338686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasma concentrations of carotenoids in healthy volunteers after intervention with carotenoid-rich foods. AU - Müller,H, AU - Bub,A, AU - Watzl,B, AU - Rechkemmer,G, PY - 1999/5/25/pubmed PY - 1999/5/25/medline PY - 1999/5/25/entrez SP - 35 EP - 44 JF - European journal of nutrition JO - Eur J Nutr VL - 38 IS - 1 N2 - AIM OF THE STUDY: The present study was conducted to investigate changes in the plasma concentration of carotenoids and carotenoid oxidation products, vitamin A, alpha- and gamma-tocopherol, and ubiquinone-10 during a dietary intervention trial with 23 male healthy volunteers. METHOD: A two week carotenoid depletion period was followed by a daily consumption of 330 mL tomato juice (40 mg lycopene), then by 330 mL carrot juice (15.7 mg alpha-carotene and 22.3 mg beta-carotene), and then by a 10 g spinach powder preparation (11.3 mg lutein and 3.1 mg beta-carotene) served with main meals for two weeks, respectively. Blood samples were collected in the morning after an overnight fasting and carotenoids, vitamin A, tocopherols, and ubichinone were analyzed by reversed-phase HPLC. RESULTS: During the tomato juice intervention, plasma concentrations of trans- and cis-lycopene increased 3-fold compared to the depletion period. Lycopene oxidation products could be demonstrated in plasma and were significantly elevated compared to control (p < 0.001). After two weeks of carrot juice consumption, alpha-carotene and beta-carotene concentrations increased 8.6- and 3.2-fold, respectively. Finally, during the spinach consumption period the lutein concentration increased 2-fold, while the beta-carotene concentrations were still elevated 2-fold. CONCLUSIONS: The moderate change in dietary habits, e.g., the consumption of 330 mL of carotenoid-rich vegetable juices caused significant changes in the plasma carotenoid concentrations, indicating a high bioavailability of carotenoids from these processed vegetable products. The changes in plasma carotenoid concentrations reflected the carotenoid composition of the consumed foods. However, particularly during the tomato juice intervention period the occurrence of lycopene oxidation products and cis-lycopene isomers in plasma was eminent. The formation may be due to antioxidant reactions of lycopene in the organism. SN - 1436-6207 UR - https://www.unboundmedicine.com/medline/citation/10338686/Plasma_concentrations_of_carotenoids_in_healthy_volunteers_after_intervention_with_carotenoid_rich_foods_ DB - PRIME DP - Unbound Medicine ER -