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In vivo and in vitro studies on the residual allergenicity of partially hydrolysed infant formulae.
Acta Paediatr 1999; 88(4):394-8AP

Abstract

AIM

Because allergen-reduced formulae are widely used in the prevention and treatment of cow's milk allergy in children and because anaphylactic reactions have been reported for some hydrolysed formulae, it is of clinical relevance to know about the residual allergenicity of so-called hypoallergenic formulae.

METHOD

We therefore studied the reactions of 20 children (mean age 1.6 years) with proven cow's milk allergy to a variety of formulae, using skin prick test, specific IgE in serum, protein content and RAST inhibition.

RESULTS

Whereas all but two children with a clinically relevant cow's milk allergy had a positive skin prick test to cow's milk, some children still showed positive responses to the partially hydrolysed formulae. No child had a positive skin test to the amino acid formula. Specific IgE to the partially hydrolysed whey formula (median 0.28 U/ml) was significantly lower (p < 0.003) than to cow's milk. Specific IgE to the partially hydrolysed whey/casein formula, soy/pork collagen hydrolysate and the amino acid formula was in a low range (median values 0.19, 0.23 and 0.21 U/ml, respectively). While determination of the protein content of the formulae gave no valid information, RAST/EAST inhibition was highest for cow's milk, followed by the partially hydrolysed whey formula, partially hydrolysed whey/casein formula, soy/pork collagen formula, and the amino acid formula.

CONCLUSION

Skin prick test and RAST inhibition test are suitable methods for determining the residual allergenicity of hydrolysed infant formulae, while determination of protein content using the applied modified Lowry method is not helpful.

Authors+Show Affiliations

University Children's Hospital Charité, Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10342536

Citation

Niggemann, B, et al. "In Vivo and in Vitro Studies On the Residual Allergenicity of Partially Hydrolysed Infant Formulae." Acta Paediatrica (Oslo, Norway : 1992), vol. 88, no. 4, 1999, pp. 394-8.
Niggemann B, Binder C, Klettke U, et al. In vivo and in vitro studies on the residual allergenicity of partially hydrolysed infant formulae. Acta Paediatr. 1999;88(4):394-8.
Niggemann, B., Binder, C., Klettke, U., & Wahn, U. (1999). In vivo and in vitro studies on the residual allergenicity of partially hydrolysed infant formulae. Acta Paediatrica (Oslo, Norway : 1992), 88(4), pp. 394-8.
Niggemann B, et al. In Vivo and in Vitro Studies On the Residual Allergenicity of Partially Hydrolysed Infant Formulae. Acta Paediatr. 1999;88(4):394-8. PubMed PMID: 10342536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo and in vitro studies on the residual allergenicity of partially hydrolysed infant formulae. AU - Niggemann,B, AU - Binder,C, AU - Klettke,U, AU - Wahn,U, PY - 1999/5/26/pubmed PY - 1999/5/26/medline PY - 1999/5/26/entrez SP - 394 EP - 8 JF - Acta paediatrica (Oslo, Norway : 1992) JO - Acta Paediatr. VL - 88 IS - 4 N2 - AIM: Because allergen-reduced formulae are widely used in the prevention and treatment of cow's milk allergy in children and because anaphylactic reactions have been reported for some hydrolysed formulae, it is of clinical relevance to know about the residual allergenicity of so-called hypoallergenic formulae. METHOD: We therefore studied the reactions of 20 children (mean age 1.6 years) with proven cow's milk allergy to a variety of formulae, using skin prick test, specific IgE in serum, protein content and RAST inhibition. RESULTS: Whereas all but two children with a clinically relevant cow's milk allergy had a positive skin prick test to cow's milk, some children still showed positive responses to the partially hydrolysed formulae. No child had a positive skin test to the amino acid formula. Specific IgE to the partially hydrolysed whey formula (median 0.28 U/ml) was significantly lower (p < 0.003) than to cow's milk. Specific IgE to the partially hydrolysed whey/casein formula, soy/pork collagen hydrolysate and the amino acid formula was in a low range (median values 0.19, 0.23 and 0.21 U/ml, respectively). While determination of the protein content of the formulae gave no valid information, RAST/EAST inhibition was highest for cow's milk, followed by the partially hydrolysed whey formula, partially hydrolysed whey/casein formula, soy/pork collagen formula, and the amino acid formula. CONCLUSION: Skin prick test and RAST inhibition test are suitable methods for determining the residual allergenicity of hydrolysed infant formulae, while determination of protein content using the applied modified Lowry method is not helpful. SN - 0803-5253 UR - https://www.unboundmedicine.com/medline/citation/10342536/In_vivo_and_in_vitro_studies_on_the_residual_allergenicity_of_partially_hydrolysed_infant_formulae_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0803-5253&amp;date=1999&amp;volume=88&amp;issue=4&amp;spage=394 DB - PRIME DP - Unbound Medicine ER -