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Temporal sequence of changes in rat retina after UV-A and blue light exposure.

Abstract

Two spectral types of retinal light damage were induced in pigmented rats by irradiating small retinal patches at either 380 or 470 nm. The temporal sequence of changes in the retina was followed for up to 2 months by funduscopy and histology. For both damage types, fundus changes were best visible after 3 days. Histology showed that 380 nm specifically damaged photoreceptor cells, particularly the rods. All cell compartments of the rods, including the nucleus were affected already after 3 h. In the next days, damaged rods degenerated. At high doses (2.5 x the funduscopic threshold dose) all rods in the irradiated area were lost, resulting in a local photoreceptor lesion, which was still present at 2 months after the irradiation. At 470 nm, damage occurred both in the photoreceptor layer and in the pigment epithelium. Acute changes, at 1 h after irradiation, consisted mainly of damaged mitochondria in these layers. Next, the pigment epithelium showed swelling, an altered melanin distribution and, at high doses (2.5 x threshold), interruptions of the monolayer. Degeneration of photoreceptor cells was initially limited to a few scattered cells, but 3 days after high doses focal areas of massive degeneration were seen. At late stages, the cells of the pigment epithelium recovered and the photoreceptor layer showed a loss of cells. The results show that the spectral damage types are distinct in the early phases, indicating that different mechanisms are involved. Yet, the end effect of both damage types after exposure at doses up to 2.5 x the funduscopic threshold is remarkably similar and consists of local photoreceptor lesions.

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  • Authors+Show Affiliations

    ,

    Department of Ophthalmology, Helmholtz Institute, Utrecht Academic Hospital, The Netherlands.

    ,

    Source

    Vision research 39:7 1999 Apr pg 1233-47

    MeSH

    Animals
    Dose-Response Relationship, Radiation
    Light
    Male
    Nerve Regeneration
    Photoreceptor Cells
    Pigment Epithelium of Eye
    Radiation Injuries
    Rats
    Rats, Long-Evans
    Retina
    Spectrophotometry
    Time Factors
    Ultraviolet Rays

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    10343838

    Citation

    Busch, E M., et al. "Temporal Sequence of Changes in Rat Retina After UV-A and Blue Light Exposure." Vision Research, vol. 39, no. 7, 1999, pp. 1233-47.
    Busch EM, Gorgels TG, van Norren D. Temporal sequence of changes in rat retina after UV-A and blue light exposure. Vision Res. 1999;39(7):1233-47.
    Busch, E. M., Gorgels, T. G., & van Norren, D. (1999). Temporal sequence of changes in rat retina after UV-A and blue light exposure. Vision Research, 39(7), pp. 1233-47.
    Busch EM, Gorgels TG, van Norren D. Temporal Sequence of Changes in Rat Retina After UV-A and Blue Light Exposure. Vision Res. 1999;39(7):1233-47. PubMed PMID: 10343838.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Temporal sequence of changes in rat retina after UV-A and blue light exposure. AU - Busch,E M, AU - Gorgels,T G, AU - van Norren,D, PY - 1999/5/27/pubmed PY - 1999/5/27/medline PY - 1999/5/27/entrez SP - 1233 EP - 47 JF - Vision research JO - Vision Res. VL - 39 IS - 7 N2 - Two spectral types of retinal light damage were induced in pigmented rats by irradiating small retinal patches at either 380 or 470 nm. The temporal sequence of changes in the retina was followed for up to 2 months by funduscopy and histology. For both damage types, fundus changes were best visible after 3 days. Histology showed that 380 nm specifically damaged photoreceptor cells, particularly the rods. All cell compartments of the rods, including the nucleus were affected already after 3 h. In the next days, damaged rods degenerated. At high doses (2.5 x the funduscopic threshold dose) all rods in the irradiated area were lost, resulting in a local photoreceptor lesion, which was still present at 2 months after the irradiation. At 470 nm, damage occurred both in the photoreceptor layer and in the pigment epithelium. Acute changes, at 1 h after irradiation, consisted mainly of damaged mitochondria in these layers. Next, the pigment epithelium showed swelling, an altered melanin distribution and, at high doses (2.5 x threshold), interruptions of the monolayer. Degeneration of photoreceptor cells was initially limited to a few scattered cells, but 3 days after high doses focal areas of massive degeneration were seen. At late stages, the cells of the pigment epithelium recovered and the photoreceptor layer showed a loss of cells. The results show that the spectral damage types are distinct in the early phases, indicating that different mechanisms are involved. Yet, the end effect of both damage types after exposure at doses up to 2.5 x the funduscopic threshold is remarkably similar and consists of local photoreceptor lesions. SN - 0042-6989 UR - https://www.unboundmedicine.com/medline/citation/10343838/Temporal_sequence_of_changes_in_rat_retina_after_UV_A_and_blue_light_exposure_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6989(98)00233-8 DB - PRIME DP - Unbound Medicine ER -