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Conjugative mobilization of the rolling-circle plasmid pIP823 from Listeria monocytogenes BM4293 among gram-positive and gram-negative bacteria.
J Bacteriol 1999; 181(11):3368-74JB

Abstract

We determined the sequence and genetic organization of plasmid pIP823, which contains the dfrD gene; dfrD confers high-level trimethoprim resistance to Listeria monocytogenes BM4293 by synthesis of dihydrofolate reductase type S2. pIP823 possessed all the features of the pUB110/pC194 plasmid family, whose members replicate by the rolling-circle mechanism. The rep gene encoded a protein identical to RepU, the protein required for initiation of the replication of plasmids pTB913 from a thermophilic Bacillus sp. and pUB110 from Staphylococcus aureus. The mob gene encoded a protein with a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmidic recombination of pTB913 and pUB110. The host range of pIP823 was broad and included L. monocytogenes, Enterococcus faecalis, S. aureus, Bacillus subtilis, and Escherichia coli. In all these species, pIP823 replicated by generating single-stranded DNA and was stable. Conjugative mobilization of pIP823 was obtained by self-transferable plasmids between L. monocytogenes and E. faecalis, between L. monocytogenes and E. coli, and between strains of E. coli, and by the streptococcal conjugative transposon Tn1545 from L. monocytogenes to E. faecalis, and from L. monocytogenes and E. faecalis to E. coli. These data indicate that the gene flux observed in nature from gram-positive to gram-negative bacteria can occur by conjugative mobilization. Our results suggest that dissemination of trimethoprim resistance in Listeria spp. and acquisition of other antibiotic resistance determinants in this species can be anticipated.

Authors+Show Affiliations

Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10348847

Citation

Charpentier, E, et al. "Conjugative Mobilization of the Rolling-circle Plasmid pIP823 From Listeria Monocytogenes BM4293 Among Gram-positive and Gram-negative Bacteria." Journal of Bacteriology, vol. 181, no. 11, 1999, pp. 3368-74.
Charpentier E, Gerbaud G, Courvalin P. Conjugative mobilization of the rolling-circle plasmid pIP823 from Listeria monocytogenes BM4293 among gram-positive and gram-negative bacteria. J Bacteriol. 1999;181(11):3368-74.
Charpentier, E., Gerbaud, G., & Courvalin, P. (1999). Conjugative mobilization of the rolling-circle plasmid pIP823 from Listeria monocytogenes BM4293 among gram-positive and gram-negative bacteria. Journal of Bacteriology, 181(11), pp. 3368-74.
Charpentier E, Gerbaud G, Courvalin P. Conjugative Mobilization of the Rolling-circle Plasmid pIP823 From Listeria Monocytogenes BM4293 Among Gram-positive and Gram-negative Bacteria. J Bacteriol. 1999;181(11):3368-74. PubMed PMID: 10348847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conjugative mobilization of the rolling-circle plasmid pIP823 from Listeria monocytogenes BM4293 among gram-positive and gram-negative bacteria. AU - Charpentier,E, AU - Gerbaud,G, AU - Courvalin,P, PY - 1999/5/29/pubmed PY - 1999/5/29/medline PY - 1999/5/29/entrez SP - 3368 EP - 74 JF - Journal of bacteriology JO - J. Bacteriol. VL - 181 IS - 11 N2 - We determined the sequence and genetic organization of plasmid pIP823, which contains the dfrD gene; dfrD confers high-level trimethoprim resistance to Listeria monocytogenes BM4293 by synthesis of dihydrofolate reductase type S2. pIP823 possessed all the features of the pUB110/pC194 plasmid family, whose members replicate by the rolling-circle mechanism. The rep gene encoded a protein identical to RepU, the protein required for initiation of the replication of plasmids pTB913 from a thermophilic Bacillus sp. and pUB110 from Staphylococcus aureus. The mob gene encoded a protein with a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmidic recombination of pTB913 and pUB110. The host range of pIP823 was broad and included L. monocytogenes, Enterococcus faecalis, S. aureus, Bacillus subtilis, and Escherichia coli. In all these species, pIP823 replicated by generating single-stranded DNA and was stable. Conjugative mobilization of pIP823 was obtained by self-transferable plasmids between L. monocytogenes and E. faecalis, between L. monocytogenes and E. coli, and between strains of E. coli, and by the streptococcal conjugative transposon Tn1545 from L. monocytogenes to E. faecalis, and from L. monocytogenes and E. faecalis to E. coli. These data indicate that the gene flux observed in nature from gram-positive to gram-negative bacteria can occur by conjugative mobilization. Our results suggest that dissemination of trimethoprim resistance in Listeria spp. and acquisition of other antibiotic resistance determinants in this species can be anticipated. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/10348847/Conjugative_mobilization_of_the_rolling_circle_plasmid_pIP823_from_Listeria_monocytogenes_BM4293_among_gram_positive_and_gram_negative_bacteria_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=10348847 DB - PRIME DP - Unbound Medicine ER -