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Detection of mutations of p53 tumor suppressor gene in pancreatic juice and its application to diagnosis of patients with pancreatic cancer: comparison with K-ras mutation.
Clin Cancer Res 1999; 5(5):1147-53CC

Abstract

Because of the difficulty in obtaining biopsy specimens from pancreatic cancer patients, K-ras mutation analysis in pancreatic juice has been used for specific diagnosis. But recently, false positives have been obtained with this method. To improve the genetic diagnosis of pancreatic cancer, detection of p53 gene mutation in pancreatic juice was studied. Pancreatic juice was sampled endoscopically. Single-strand conformation polymorphism analysis was used for p53 mutation analysis. Furthermore, K-ras mutations at codon 12 were also studied in the same pancreatic cancer patients. Of 26 cases of pancreatic cancer, p53 mutations were detected in 11 (42.3%). No mutations were seen in the cases with mucin-producing adenoma nor with chronic pancreatitis. K-ras mutations were detected in 84.0% of cases by RFLP analysis, which has high sensitivity, and in 65.3% by hybridization protection assay, which has high specificity. Using a combination assay with both genes, genetic abnormalities were detected in 92.0% by RFLP and 73.1% by hybridization protection assay including two cases in which p53 alone was positive by both methods. The specificity of p53 mutation for pancreatic cancer is very high. Therefore, simultaneous analysis of p53 and K-ras mutation is suggested to enhance the genetic diagnosis of pancreatic cancer.

Authors+Show Affiliations

Department of Internal Medicine and Medical Oncology, Cancer Research Institute, Kanazawa University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10353750

Citation

Yamaguchi, Y, et al. "Detection of Mutations of P53 Tumor Suppressor Gene in Pancreatic Juice and Its Application to Diagnosis of Patients With Pancreatic Cancer: Comparison With K-ras Mutation." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 5, no. 5, 1999, pp. 1147-53.
Yamaguchi Y, Watanabe H, Yrdiran S, et al. Detection of mutations of p53 tumor suppressor gene in pancreatic juice and its application to diagnosis of patients with pancreatic cancer: comparison with K-ras mutation. Clin Cancer Res. 1999;5(5):1147-53.
Yamaguchi, Y., Watanabe, H., Yrdiran, S., Ohtsubo, K., Motoo, Y., Okai, T., & Sawabu, N. (1999). Detection of mutations of p53 tumor suppressor gene in pancreatic juice and its application to diagnosis of patients with pancreatic cancer: comparison with K-ras mutation. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 5(5), pp. 1147-53.
Yamaguchi Y, et al. Detection of Mutations of P53 Tumor Suppressor Gene in Pancreatic Juice and Its Application to Diagnosis of Patients With Pancreatic Cancer: Comparison With K-ras Mutation. Clin Cancer Res. 1999;5(5):1147-53. PubMed PMID: 10353750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of mutations of p53 tumor suppressor gene in pancreatic juice and its application to diagnosis of patients with pancreatic cancer: comparison with K-ras mutation. AU - Yamaguchi,Y, AU - Watanabe,H, AU - Yrdiran,S, AU - Ohtsubo,K, AU - Motoo,Y, AU - Okai,T, AU - Sawabu,N, PY - 1999/6/3/pubmed PY - 1999/6/3/medline PY - 1999/6/3/entrez SP - 1147 EP - 53 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin. Cancer Res. VL - 5 IS - 5 N2 - Because of the difficulty in obtaining biopsy specimens from pancreatic cancer patients, K-ras mutation analysis in pancreatic juice has been used for specific diagnosis. But recently, false positives have been obtained with this method. To improve the genetic diagnosis of pancreatic cancer, detection of p53 gene mutation in pancreatic juice was studied. Pancreatic juice was sampled endoscopically. Single-strand conformation polymorphism analysis was used for p53 mutation analysis. Furthermore, K-ras mutations at codon 12 were also studied in the same pancreatic cancer patients. Of 26 cases of pancreatic cancer, p53 mutations were detected in 11 (42.3%). No mutations were seen in the cases with mucin-producing adenoma nor with chronic pancreatitis. K-ras mutations were detected in 84.0% of cases by RFLP analysis, which has high sensitivity, and in 65.3% by hybridization protection assay, which has high specificity. Using a combination assay with both genes, genetic abnormalities were detected in 92.0% by RFLP and 73.1% by hybridization protection assay including two cases in which p53 alone was positive by both methods. The specificity of p53 mutation for pancreatic cancer is very high. Therefore, simultaneous analysis of p53 and K-ras mutation is suggested to enhance the genetic diagnosis of pancreatic cancer. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/10353750/Detection_of_mutations_of_p53_tumor_suppressor_gene_in_pancreatic_juice_and_its_application_to_diagnosis_of_patients_with_pancreatic_cancer:_comparison_with_K_ras_mutation_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=10353750 DB - PRIME DP - Unbound Medicine ER -