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Apoptosis and the pattern of DNase I expression following massive small bowel resection.
J Surg Res. 1999 Jun 15; 84(2):218-22.JS

Abstract

INTRODUCTION

Following massive small bowel resection (SBR), histologic evidence of increased enterocyte apoptosis has been demonstrated in several animal models. Deoxyribonuclease I (DNase I) is requisite for intranuclear cleavage of DNA during apoptosis; we therefore hypothesized that the expression of this gene would be increased following SBR.

METHODS

Male ICR mice underwent either 50% proximal SBR or sham surgery (bowel transection/reanastomosis). After 12 h and 1, 3, and 7 days, rates of enterocyte proliferation and apoptosis were recorded as well as DNase I mRNA expression and activity.

RESULTS

Adaptation after SBR was confirmed at each time point by increased proliferation. Enterocyte proliferation was increased by 12 h and apoptosis was increased by 24 h and remained elevated through Day 7. When compared with sham-operated mice, SBR resulted in a twofold increase in both DNase I expression and activity at 24 h postoperatively, which returned to baseline by Postoperative Day 3.

CONCLUSIONS

DNase I expression and activity are increased early following massive SBR but return to baseline despite persistent increased rates of enterocyte apoptosis and proliferation. This enzyme may be important in the early induction of apoptosis following massive SBR, but not once a new set point has been established in the balance between the rate of enterocyte production and enterocyte loss.

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Authors+Show Affiliations

Falcone RA
Department of Surgery, Children's Hospital Medical Center, Cincinnati, Ohio, 45229-3039, USA.
Stern LE
No affiliation info available
Kemp CJ
No affiliation info available
Shin CE
No affiliation info available
Erwin CR
No affiliation info available
Warner BW
No affiliation info available

MeSH

Adaptation, PhysiologicalAnimalsApoptosisCell DivisionDeoxyribonuclease IIntestine, SmallMaleMiceMice, Inbred ICRPostoperative PeriodRNA, MessengerTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10357923

Citation

Falcone, R A., et al. "Apoptosis and the Pattern of DNase I Expression Following Massive Small Bowel Resection." The Journal of Surgical Research, vol. 84, no. 2, 1999, pp. 218-22.
Falcone RA, Stern LE, Kemp CJ, et al. Apoptosis and the pattern of DNase I expression following massive small bowel resection. J Surg Res. 1999;84(2):218-22.
Falcone, R. A., Stern, L. E., Kemp, C. J., Shin, C. E., Erwin, C. R., & Warner, B. W. (1999). Apoptosis and the pattern of DNase I expression following massive small bowel resection. The Journal of Surgical Research, 84(2), 218-22.
Falcone RA, et al. Apoptosis and the Pattern of DNase I Expression Following Massive Small Bowel Resection. J Surg Res. 1999 Jun 15;84(2):218-22. PubMed PMID: 10357923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis and the pattern of DNase I expression following massive small bowel resection. AU - Falcone,R A,Jr AU - Stern,L E, AU - Kemp,C J, AU - Shin,C E, AU - Erwin,C R, AU - Warner,B W, PY - 1999/6/8/pubmed PY - 2001/3/28/medline PY - 1999/6/8/entrez SP - 218 EP - 22 JF - The Journal of surgical research JO - J Surg Res VL - 84 IS - 2 N2 - INTRODUCTION: Following massive small bowel resection (SBR), histologic evidence of increased enterocyte apoptosis has been demonstrated in several animal models. Deoxyribonuclease I (DNase I) is requisite for intranuclear cleavage of DNA during apoptosis; we therefore hypothesized that the expression of this gene would be increased following SBR. METHODS: Male ICR mice underwent either 50% proximal SBR or sham surgery (bowel transection/reanastomosis). After 12 h and 1, 3, and 7 days, rates of enterocyte proliferation and apoptosis were recorded as well as DNase I mRNA expression and activity. RESULTS: Adaptation after SBR was confirmed at each time point by increased proliferation. Enterocyte proliferation was increased by 12 h and apoptosis was increased by 24 h and remained elevated through Day 7. When compared with sham-operated mice, SBR resulted in a twofold increase in both DNase I expression and activity at 24 h postoperatively, which returned to baseline by Postoperative Day 3. CONCLUSIONS: DNase I expression and activity are increased early following massive SBR but return to baseline despite persistent increased rates of enterocyte apoptosis and proliferation. This enzyme may be important in the early induction of apoptosis following massive SBR, but not once a new set point has been established in the balance between the rate of enterocyte production and enterocyte loss. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/10357923/Apoptosis_and_the_pattern_of_DNase_I_expression_following_massive_small_bowel_resection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(99)95649-4 DB - PRIME DP - Unbound Medicine ER -