Tags

Type your tag names separated by a space and hit enter

Deposition of eosinophil-granule major basic protein and expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in the mucosa of the small intestine in infants with cow's milk-sensitive enteropathy.
J Allergy Clin Immunol 1999; 103(6):1195-201JA

Abstract

BACKGROUND

Cow's milk-sensitive enteropathy (CMSE) is an important cause of chronic diarrhea and failure to thrive in infancy. The immunopathology of the mucosal lesion associated with CMSE has not yet been described.

OBJECTIVES

This study investigated the eosinophil activation and the role of adhesion molecules in the pathogenesis of intestinal mucosal damage associated with CMSE.

METHODS

Twenty-one patients with chronic diarrhea and abnormal mucosa on duodenal biopsy specimens were included. The patients had negative responses to skin prick tests and RASTs with milk. Fourteen patients were diagnosed with CMSE by milk challenge test and were designated as the CMSE group. Seven patients with no milk intolerance were defined as the non-CMSE group. Four infants with frequent vomiting and no mucosal abnormalities were also studied as the control group. Immunohistochemical stains for eosinophil major basic protein (MBP), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 on endoscopic duodenal biopsy specimens were performed.

RESULTS

The degree of eosinophil degranulation, as evidenced by localization of extracellular MBP, was significantly greater in the CMSE group compared with the non-CMSE and control groups (P <.05). Expression of VCAM-1 on mononuclear cells was higher in the CMSE group compared with the non-CMSE and control groups (P <.05). The severity of villous atrophy was positively correlated with the deposition of MBP (r = 0.79, P <.001).

CONCLUSION

These results strongly suggest eosinophils and VCAM-1 are implicated in the pathogenesis of mucosal damage associated with CMSE.

Authors+Show Affiliations

Departments of Pediatrics and Pathology, School of Medicine, Catholic University of Taegu-Hyosung, Taegu, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10359906

Citation

Chung, H L., et al. "Deposition of Eosinophil-granule Major Basic Protein and Expression of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 in the Mucosa of the Small Intestine in Infants With Cow's Milk-sensitive Enteropathy." The Journal of Allergy and Clinical Immunology, vol. 103, no. 6, 1999, pp. 1195-201.
Chung HL, Hwang JB, Kwon YD, et al. Deposition of eosinophil-granule major basic protein and expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in the mucosa of the small intestine in infants with cow's milk-sensitive enteropathy. J Allergy Clin Immunol. 1999;103(6):1195-201.
Chung, H. L., Hwang, J. B., Kwon, Y. D., Park, M. H., Shin, W. J., & Park, J. B. (1999). Deposition of eosinophil-granule major basic protein and expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in the mucosa of the small intestine in infants with cow's milk-sensitive enteropathy. The Journal of Allergy and Clinical Immunology, 103(6), pp. 1195-201.
Chung HL, et al. Deposition of Eosinophil-granule Major Basic Protein and Expression of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 in the Mucosa of the Small Intestine in Infants With Cow's Milk-sensitive Enteropathy. J Allergy Clin Immunol. 1999;103(6):1195-201. PubMed PMID: 10359906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deposition of eosinophil-granule major basic protein and expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in the mucosa of the small intestine in infants with cow's milk-sensitive enteropathy. AU - Chung,H L, AU - Hwang,J B, AU - Kwon,Y D, AU - Park,M H, AU - Shin,W J, AU - Park,J B, PY - 1999/6/9/pubmed PY - 1999/6/9/medline PY - 1999/6/9/entrez SP - 1195 EP - 201 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 103 IS - 6 N2 - BACKGROUND: Cow's milk-sensitive enteropathy (CMSE) is an important cause of chronic diarrhea and failure to thrive in infancy. The immunopathology of the mucosal lesion associated with CMSE has not yet been described. OBJECTIVES: This study investigated the eosinophil activation and the role of adhesion molecules in the pathogenesis of intestinal mucosal damage associated with CMSE. METHODS: Twenty-one patients with chronic diarrhea and abnormal mucosa on duodenal biopsy specimens were included. The patients had negative responses to skin prick tests and RASTs with milk. Fourteen patients were diagnosed with CMSE by milk challenge test and were designated as the CMSE group. Seven patients with no milk intolerance were defined as the non-CMSE group. Four infants with frequent vomiting and no mucosal abnormalities were also studied as the control group. Immunohistochemical stains for eosinophil major basic protein (MBP), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 on endoscopic duodenal biopsy specimens were performed. RESULTS: The degree of eosinophil degranulation, as evidenced by localization of extracellular MBP, was significantly greater in the CMSE group compared with the non-CMSE and control groups (P <.05). Expression of VCAM-1 on mononuclear cells was higher in the CMSE group compared with the non-CMSE and control groups (P <.05). The severity of villous atrophy was positively correlated with the deposition of MBP (r = 0.79, P <.001). CONCLUSION: These results strongly suggest eosinophils and VCAM-1 are implicated in the pathogenesis of mucosal damage associated with CMSE. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/10359906/Deposition_of_eosinophil_granule_major_basic_protein_and_expression_of_intercellular_adhesion_molecule_1_and_vascular_cell_adhesion_molecule_1_in_the_mucosa_of_the_small_intestine_in_infants_with_cow's_milk_sensitive_enteropathy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(99)70199-5 DB - PRIME DP - Unbound Medicine ER -