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Relation between vacA subtypes, cytotoxin activity, and disease in Helicobacter pylori-infected patients from The Netherlands.
Am J Gastroenterol. 1999 Jun; 94(6):1517-21.AJ

Abstract

OBJECTIVE

The vacuolating cytotoxin of Helicobacter pylori (H. pylori) is encoded by vacA, of which allelic variation has been described. In the U.S., H. pylori strains with the signal sequence allele s1a are associated with enhanced gastric inflammation and with peptic ulcer disease (PUD). The m1 middle region allele is linked with more severe gastric epithelial damage. However, the distribution of H. pylori genotypes and the association with disease may be different in other geographical areas. The aim of this study was to establish whether vacA types among H. pylori isolates from Dutch patients are associated with disease.

METHODS

The cytotoxin activity of the H. pylori isolates from 34 PUD patients and 46 patients with functional dyspepsia (FD) was assessed by an in vitro assay using HeLa cells as indicator cells. The vacA types and cagA status of the isolates were assessed by polymerase chain reaction (PCR).

RESULTS

vacA s1-type H. pylori displayed cytotoxin activity more frequently than s2 vacA-type H. pylori (p = 0.003). This difference was not significant when only cagA+ H. pylori were considered. H. pylori isolates with the m1 vacA type exhibited a higher cytotoxin activity, independent of cagA (p = 0.006). Ninety-four percent (32/34) of the PUD patients and 74% (34/46) of the FD patients were infected with s1 vacA-type H. pylori (p = 0.04). When only cagA+ H. pylori were considered, s1 vacA type was not associated with disease. In addition, neither the s1a nor s1b subtypes correlated with disease.

CONCLUSIONS

An association between vacA subtypes and disease could not be established in this patient population, due to the strong linkage between vacA s1 type and cagA.

Authors+Show Affiliations

Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10364017

Citation

Pan, Z J., et al. "Relation Between vacA Subtypes, Cytotoxin Activity, and Disease in Helicobacter Pylori-infected Patients From the Netherlands." The American Journal of Gastroenterology, vol. 94, no. 6, 1999, pp. 1517-21.
Pan ZJ, van der Hulst RW, Tytgat GN, et al. Relation between vacA subtypes, cytotoxin activity, and disease in Helicobacter pylori-infected patients from The Netherlands. Am J Gastroenterol. 1999;94(6):1517-21.
Pan, Z. J., van der Hulst, R. W., Tytgat, G. N., Dankert, J., & van der Ende, A. (1999). Relation between vacA subtypes, cytotoxin activity, and disease in Helicobacter pylori-infected patients from The Netherlands. The American Journal of Gastroenterology, 94(6), 1517-21.
Pan ZJ, et al. Relation Between vacA Subtypes, Cytotoxin Activity, and Disease in Helicobacter Pylori-infected Patients From the Netherlands. Am J Gastroenterol. 1999;94(6):1517-21. PubMed PMID: 10364017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relation between vacA subtypes, cytotoxin activity, and disease in Helicobacter pylori-infected patients from The Netherlands. AU - Pan,Z J, AU - van der Hulst,R W, AU - Tytgat,G N, AU - Dankert,J, AU - van der Ende,A, PY - 1999/6/11/pubmed PY - 1999/6/11/medline PY - 1999/6/11/entrez SP - 1517 EP - 21 JF - The American journal of gastroenterology JO - Am J Gastroenterol VL - 94 IS - 6 N2 - OBJECTIVE: The vacuolating cytotoxin of Helicobacter pylori (H. pylori) is encoded by vacA, of which allelic variation has been described. In the U.S., H. pylori strains with the signal sequence allele s1a are associated with enhanced gastric inflammation and with peptic ulcer disease (PUD). The m1 middle region allele is linked with more severe gastric epithelial damage. However, the distribution of H. pylori genotypes and the association with disease may be different in other geographical areas. The aim of this study was to establish whether vacA types among H. pylori isolates from Dutch patients are associated with disease. METHODS: The cytotoxin activity of the H. pylori isolates from 34 PUD patients and 46 patients with functional dyspepsia (FD) was assessed by an in vitro assay using HeLa cells as indicator cells. The vacA types and cagA status of the isolates were assessed by polymerase chain reaction (PCR). RESULTS: vacA s1-type H. pylori displayed cytotoxin activity more frequently than s2 vacA-type H. pylori (p = 0.003). This difference was not significant when only cagA+ H. pylori were considered. H. pylori isolates with the m1 vacA type exhibited a higher cytotoxin activity, independent of cagA (p = 0.006). Ninety-four percent (32/34) of the PUD patients and 74% (34/46) of the FD patients were infected with s1 vacA-type H. pylori (p = 0.04). When only cagA+ H. pylori were considered, s1 vacA type was not associated with disease. In addition, neither the s1a nor s1b subtypes correlated with disease. CONCLUSIONS: An association between vacA subtypes and disease could not be established in this patient population, due to the strong linkage between vacA s1 type and cagA. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/10364017/Relation_between_vacA_subtypes_cytotoxin_activity_and_disease_in_Helicobacter_pylori_infected_patients_from_The_Netherlands_ L2 - https://Insights.ovid.com/pubmed?pmid=10364017 DB - PRIME DP - Unbound Medicine ER -