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Increased production of vascular endothelial growth factor by intestinal mucosa of patients with inflammatory bowel disease.
Hepatogastroenterology 1999 Mar-Apr; 46(26):920-3H

Abstract

BACKGROUND/AIMS

Vascular endothelial growth factor (VEGF) is a heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. Recent studies have shown significantly increased VEGF serum levels in patients with active Crohn's disease and ulcerative colitis. The origin of the circulating VEGF is not yet completely described. The present investigation examines the VEGF production of colonic mucosa in consideration of mucosal disease activity in patients with inflammatory bowel disease.

METHODOLOGY

Fifteen patients with inflammatory bowel disease were studied, 9 patients with Crohn's disease and 6 patients with ulcerative colitis. Biopsies were taken from endoscopically inflamed and non-inflamed colonic mucosa. Therefore, an analysis of the spontaneous VEGF production of cultured biopsies without stimulus and of the histological grade of inflammation scored on a scale of 0-3 (normal mucosa--severe chronic colitis) were performed. Eight patients with irritable bowel syndrome served as controls. VEGF levels in the supernatant of cultured mucosal biopsies were measured using an enzyme linked immunosorbent assay.

RESULTS

VEGF production is expressed as pg/mg wet weight of the biopsies. Inflamed mucosa of patients with active ulcerative colitis (16.27 +/- 10.39, p = 0.003, n = 6) and active Crohn's disease (9.88 +/- 5.98, p < 0.012, n = 9) showed a significantly higher spontaneous production of VEGF by colonic mucosa than normal mucosa of controls (3.16 +/- 1.63, n = 8). In addition, there was an increased unstimulated VEGF production by cultured inflamed mucosa of patients with Crohn's disease compared with non-inflamed mucosa (3.88 +/- 3.66, p < 0.015, n = 9). In both Crohn's disease and ulcerative colitis, there was no significant difference between VEGF production by non-inflamed mucosa and normal mucosa of controls.

CONCLUSIONS

The present study identifies the intestinal mucosa as one of the origins of the elevated VEGF serum levels in patients with active inflammatory bowel disease and verifies the findings of recent studies about the importance of VEGF in Crohn's disease and ulcerative colitis.

Authors+Show Affiliations

Department of Gastroenterology, University of Bochum, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10370639

Citation

Griga, T, et al. "Increased Production of Vascular Endothelial Growth Factor By Intestinal Mucosa of Patients With Inflammatory Bowel Disease." Hepato-gastroenterology, vol. 46, no. 26, 1999, pp. 920-3.
Griga T, Voigt E, Gretzer B, et al. Increased production of vascular endothelial growth factor by intestinal mucosa of patients with inflammatory bowel disease. Hepatogastroenterology. 1999;46(26):920-3.
Griga, T., Voigt, E., Gretzer, B., Brasch, F., & May, B. (1999). Increased production of vascular endothelial growth factor by intestinal mucosa of patients with inflammatory bowel disease. Hepato-gastroenterology, 46(26), pp. 920-3.
Griga T, et al. Increased Production of Vascular Endothelial Growth Factor By Intestinal Mucosa of Patients With Inflammatory Bowel Disease. Hepatogastroenterology. 1999;46(26):920-3. PubMed PMID: 10370639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased production of vascular endothelial growth factor by intestinal mucosa of patients with inflammatory bowel disease. AU - Griga,T, AU - Voigt,E, AU - Gretzer,B, AU - Brasch,F, AU - May,B, PY - 1999/6/17/pubmed PY - 1999/6/17/medline PY - 1999/6/17/entrez SP - 920 EP - 3 JF - Hepato-gastroenterology JO - Hepatogastroenterology VL - 46 IS - 26 N2 - BACKGROUND/AIMS: Vascular endothelial growth factor (VEGF) is a heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. Recent studies have shown significantly increased VEGF serum levels in patients with active Crohn's disease and ulcerative colitis. The origin of the circulating VEGF is not yet completely described. The present investigation examines the VEGF production of colonic mucosa in consideration of mucosal disease activity in patients with inflammatory bowel disease. METHODOLOGY: Fifteen patients with inflammatory bowel disease were studied, 9 patients with Crohn's disease and 6 patients with ulcerative colitis. Biopsies were taken from endoscopically inflamed and non-inflamed colonic mucosa. Therefore, an analysis of the spontaneous VEGF production of cultured biopsies without stimulus and of the histological grade of inflammation scored on a scale of 0-3 (normal mucosa--severe chronic colitis) were performed. Eight patients with irritable bowel syndrome served as controls. VEGF levels in the supernatant of cultured mucosal biopsies were measured using an enzyme linked immunosorbent assay. RESULTS: VEGF production is expressed as pg/mg wet weight of the biopsies. Inflamed mucosa of patients with active ulcerative colitis (16.27 +/- 10.39, p = 0.003, n = 6) and active Crohn's disease (9.88 +/- 5.98, p < 0.012, n = 9) showed a significantly higher spontaneous production of VEGF by colonic mucosa than normal mucosa of controls (3.16 +/- 1.63, n = 8). In addition, there was an increased unstimulated VEGF production by cultured inflamed mucosa of patients with Crohn's disease compared with non-inflamed mucosa (3.88 +/- 3.66, p < 0.015, n = 9). In both Crohn's disease and ulcerative colitis, there was no significant difference between VEGF production by non-inflamed mucosa and normal mucosa of controls. CONCLUSIONS: The present study identifies the intestinal mucosa as one of the origins of the elevated VEGF serum levels in patients with active inflammatory bowel disease and verifies the findings of recent studies about the importance of VEGF in Crohn's disease and ulcerative colitis. SN - 0172-6390 UR - https://www.unboundmedicine.com/medline/citation/10370639/Increased_production_of_vascular_endothelial_growth_factor_by_intestinal_mucosa_of_patients_with_inflammatory_bowel_disease_ L2 - https://medlineplus.gov/crohnsdisease.html DB - PRIME DP - Unbound Medicine ER -