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Effect of oral supplementation with vitamin E on the oxido-reductive status of red blood cells in normal mice and mice subject to oxidative stress by chronic administration of adriamycin.
Ann Clin Biochem. 1999 Mar; 36 (Pt 2):216-20.AC

Abstract

The effect of oral vitamin E supplementation on the oxido-reductive status of red blood cells in normal mice and those subject to oxidative stress by chronic administration of the anti-tumour drug Adriamycin was investigated. Mice were randomly separated into three groups of 20 animals each and maintained on diets identical in all respects except for vitamin E content. Group 1 received a low vitamin E diet that provided 10 mg vitamin E/kg body weight/day, group 2 received a normal mice chow diet (45 mg vitamin E/kg body weight/day) while group 3 received a high vitamin E diet (200 mg vitamin E/kg body weight/day). In comparison with the normal mice in group 1, their counterparts in groups 2 and 3 exhibited significantly higher (P < 0.001) activities of superoxide dismutase (SOD) in red blood cells (79.4% higher in group 2 and 114.2% higher in group 3, respectively) and produced lower concentrations of malondialdehyde (MDA) (22.9% less in group 2 and 51.2% less in group 3, respectively), with little difference in the glutathione peroxidase (GPX) activity. In Adriamycin-treated animals on the low vitamin E diet (group 1) the red blood cell SOD activity and MDA production were 46.2% and 200.7% higher (P < 0.001), respectively, and the GPX activity was 39.1% lower than in the red blood cells of untreated (normal) animals in the same group. The Adriamycin-induced changes were significantly less in animals receiving higher doses of vitamin E (groups 2 and 3). Thus, in the group maintained on the high vitamin E diet (group 3), Adriamycin administration resulted in only a 38.9% increase in the MDA production above that generated by red blood cells of normal mice in the same group, with no significant change in the SOD or GPX activities. Thus, in normal conditions as well as in conditions of oxidative stress, high doses of vitamin E appear to be able to protect the oxido-reductive status of red blood cells by modulating the extent of lipid peroxidation as well as the activities of antioxidant enzymes.

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Authors+Show Affiliations

Thabrew MI
Department of Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka. mrthab@sri.lanka.net
Samarawickrema N
No affiliation info available
Chandrasena LG
No affiliation info available
Jayasekera S
No affiliation info available

MeSH

Administration, OralAnimalsAntibiotics, AntineoplasticDoxorubicinErythrocytesGlutathione PeroxidaseLipid PeroxidationMaleMalondialdehydeMiceOxidation-ReductionOxidative StressSuperoxide DismutaseVitamin E

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10370740

Citation

Thabrew, M I., et al. "Effect of Oral Supplementation With Vitamin E On the Oxido-reductive Status of Red Blood Cells in Normal Mice and Mice Subject to Oxidative Stress By Chronic Administration of Adriamycin." Annals of Clinical Biochemistry, vol. 36 (Pt 2), 1999, pp. 216-20.
Thabrew MI, Samarawickrema N, Chandrasena LG, et al. Effect of oral supplementation with vitamin E on the oxido-reductive status of red blood cells in normal mice and mice subject to oxidative stress by chronic administration of adriamycin. Ann Clin Biochem. 1999;36 (Pt 2):216-20.
Thabrew, M. I., Samarawickrema, N., Chandrasena, L. G., & Jayasekera, S. (1999). Effect of oral supplementation with vitamin E on the oxido-reductive status of red blood cells in normal mice and mice subject to oxidative stress by chronic administration of adriamycin. Annals of Clinical Biochemistry, 36 (Pt 2), 216-20.
Thabrew MI, et al. Effect of Oral Supplementation With Vitamin E On the Oxido-reductive Status of Red Blood Cells in Normal Mice and Mice Subject to Oxidative Stress By Chronic Administration of Adriamycin. Ann Clin Biochem. 1999;36 (Pt 2):216-20. PubMed PMID: 10370740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of oral supplementation with vitamin E on the oxido-reductive status of red blood cells in normal mice and mice subject to oxidative stress by chronic administration of adriamycin. AU - Thabrew,M I, AU - Samarawickrema,N, AU - Chandrasena,L G, AU - Jayasekera,S, PY - 1999/6/17/pubmed PY - 1999/6/17/medline PY - 1999/6/17/entrez SP - 216 EP - 20 JF - Annals of clinical biochemistry JO - Ann Clin Biochem VL - 36 (Pt 2) N2 - The effect of oral vitamin E supplementation on the oxido-reductive status of red blood cells in normal mice and those subject to oxidative stress by chronic administration of the anti-tumour drug Adriamycin was investigated. Mice were randomly separated into three groups of 20 animals each and maintained on diets identical in all respects except for vitamin E content. Group 1 received a low vitamin E diet that provided 10 mg vitamin E/kg body weight/day, group 2 received a normal mice chow diet (45 mg vitamin E/kg body weight/day) while group 3 received a high vitamin E diet (200 mg vitamin E/kg body weight/day). In comparison with the normal mice in group 1, their counterparts in groups 2 and 3 exhibited significantly higher (P < 0.001) activities of superoxide dismutase (SOD) in red blood cells (79.4% higher in group 2 and 114.2% higher in group 3, respectively) and produced lower concentrations of malondialdehyde (MDA) (22.9% less in group 2 and 51.2% less in group 3, respectively), with little difference in the glutathione peroxidase (GPX) activity. In Adriamycin-treated animals on the low vitamin E diet (group 1) the red blood cell SOD activity and MDA production were 46.2% and 200.7% higher (P < 0.001), respectively, and the GPX activity was 39.1% lower than in the red blood cells of untreated (normal) animals in the same group. The Adriamycin-induced changes were significantly less in animals receiving higher doses of vitamin E (groups 2 and 3). Thus, in the group maintained on the high vitamin E diet (group 3), Adriamycin administration resulted in only a 38.9% increase in the MDA production above that generated by red blood cells of normal mice in the same group, with no significant change in the SOD or GPX activities. Thus, in normal conditions as well as in conditions of oxidative stress, high doses of vitamin E appear to be able to protect the oxido-reductive status of red blood cells by modulating the extent of lipid peroxidation as well as the activities of antioxidant enzymes. SN - 0004-5632 UR - https://www.unboundmedicine.com/medline/citation/10370740/Effect_of_oral_supplementation_with_vitamin_E_on_the_oxido_reductive_status_of_red_blood_cells_in_normal_mice_and_mice_subject_to_oxidative_stress_by_chronic_administration_of_adriamycin_ L2 - https://journals.sagepub.com/doi/10.1177/000456329903600214?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -