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Frequent Bax frameshift mutations in gastric cancer with high but not low microsatellite instability.
J Exp Clin Cancer Res. 1999 Mar; 18(1):103-6.JE

Abstract

Widespread microsatellite instability (MSI) due to the defective DNA mismatch repair underlies the pathogenesis of the majority of hereditary non-polyposis colorectal cancer and a subset of various sporadic malignant tumors. Using 5 microsatellite markers and the criteria of MSI proposed by the National Cancer Institute (NCI) workshop, we analyzed 205 gastric adenocarcinomas for MSI. Based on the number of markers showing instability per tumor, the tumors were divided into three groups; those with two or more of the five markers displaying instability (high MSI, MSI-H), those with one of five markers displaying instability (low MSI, MSI-L), and those with no instability (microsatellite stable, MSS). Among 205 tumors, 30 (15%) were MSI-H, 15 (7%) were MSI-L, and 160 (78%) were MSS. All of the 30 MSI-H tumors demonstrated instability at BAT26, a sensitive marker for the widespread MSI, while none of the 15 MSI-L tumors did. MSI-H tumors were significantly associated with distal location and well or moderate differentiation, but MSI-L tumors were indistinguishable from MSS tumors. Bax frameshift mutations were detected in 60% of the 30 MSI-H tumors, while not in any of the 15 MSI-L tumors. These results suggest that microsatellite analysis using the criteria proposed by the NCI workshop may be appropriate for gastric cancers because it unveils real differences in genotype and phenotype.

Authors+Show Affiliations

First Dept. of Internal Medicine, Sapporo Medical University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10374688

Citation

Yamamoto, H, et al. "Frequent Bax Frameshift Mutations in Gastric Cancer With High but Not Low Microsatellite Instability." Journal of Experimental & Clinical Cancer Research : CR, vol. 18, no. 1, 1999, pp. 103-6.
Yamamoto H, Itoh F, Fukushima H, et al. Frequent Bax frameshift mutations in gastric cancer with high but not low microsatellite instability. J Exp Clin Cancer Res. 1999;18(1):103-6.
Yamamoto, H., Itoh, F., Fukushima, H., Adachi, Y., Itoh, H., Hinoda, Y., & Imai, K. (1999). Frequent Bax frameshift mutations in gastric cancer with high but not low microsatellite instability. Journal of Experimental & Clinical Cancer Research : CR, 18(1), 103-6.
Yamamoto H, et al. Frequent Bax Frameshift Mutations in Gastric Cancer With High but Not Low Microsatellite Instability. J Exp Clin Cancer Res. 1999;18(1):103-6. PubMed PMID: 10374688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequent Bax frameshift mutations in gastric cancer with high but not low microsatellite instability. AU - Yamamoto,H, AU - Itoh,F, AU - Fukushima,H, AU - Adachi,Y, AU - Itoh,H, AU - Hinoda,Y, AU - Imai,K, PY - 1999/6/22/pubmed PY - 1999/6/22/medline PY - 1999/6/22/entrez SP - 103 EP - 6 JF - Journal of experimental & clinical cancer research : CR JO - J Exp Clin Cancer Res VL - 18 IS - 1 N2 - Widespread microsatellite instability (MSI) due to the defective DNA mismatch repair underlies the pathogenesis of the majority of hereditary non-polyposis colorectal cancer and a subset of various sporadic malignant tumors. Using 5 microsatellite markers and the criteria of MSI proposed by the National Cancer Institute (NCI) workshop, we analyzed 205 gastric adenocarcinomas for MSI. Based on the number of markers showing instability per tumor, the tumors were divided into three groups; those with two or more of the five markers displaying instability (high MSI, MSI-H), those with one of five markers displaying instability (low MSI, MSI-L), and those with no instability (microsatellite stable, MSS). Among 205 tumors, 30 (15%) were MSI-H, 15 (7%) were MSI-L, and 160 (78%) were MSS. All of the 30 MSI-H tumors demonstrated instability at BAT26, a sensitive marker for the widespread MSI, while none of the 15 MSI-L tumors did. MSI-H tumors were significantly associated with distal location and well or moderate differentiation, but MSI-L tumors were indistinguishable from MSS tumors. Bax frameshift mutations were detected in 60% of the 30 MSI-H tumors, while not in any of the 15 MSI-L tumors. These results suggest that microsatellite analysis using the criteria proposed by the NCI workshop may be appropriate for gastric cancers because it unveils real differences in genotype and phenotype. SN - 0392-9078 UR - https://www.unboundmedicine.com/medline/citation/10374688/Frequent_Bax_frameshift_mutations_in_gastric_cancer_with_high_but_not_low_microsatellite_instability_ L2 - https://medlineplus.gov/stomachcancer.html DB - PRIME DP - Unbound Medicine ER -