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Characteristics of transient outward K+ current in human atrial cardiomyocytes.
Zhongguo Yao Li Xue Bao. 1998 Sep; 19(5):481-5.ZY

Abstract

AIM

To study the properties of transient outward potassium current (Ito) in human atrial myocytes.

METHODS

The patch-clamp whole-cell recording techniques were used. When the calcium inward current was blocked by CdCl2 (0.1 mmol.L-1), the transient outward potassium current (Ito) was recorded by depolarizing cells.

RESULTS

The current was voltage dependent, it was activated quickly and inactivated rapidly too. 4-Aminopyridine (4-AP) 10 mmol.L-1 (a selective blocker of Ito) blocked the current completely. The IC50 (95% confidence limits) of 4-AP on Ito were 0.67 (0.58-0.80) mmol.L-1, 4-AP 1 mmol.L-1 shifted the activation curve of Ito to positive potential, therefore a higher membrane potential was required to activate Ito.

CONCLUSION

Ito, a major K+ channel current in human atrial cells, is blocked by 4-AP.

Authors+Show Affiliations

Department of Pharmacology, Peking Union Medical College, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10375816

Citation

Xu, W H., et al. "Characteristics of Transient Outward K+ Current in Human Atrial Cardiomyocytes." Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica, vol. 19, no. 5, 1998, pp. 481-5.
Xu WH, Li W, Yue HW, et al. Characteristics of transient outward K+ current in human atrial cardiomyocytes. Zhongguo Yao Li Xue Bao. 1998;19(5):481-5.
Xu, W. H., Li, W., Yue, H. W., & Wang, X. L. (1998). Characteristics of transient outward K+ current in human atrial cardiomyocytes. Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica, 19(5), 481-5.
Xu WH, et al. Characteristics of Transient Outward K+ Current in Human Atrial Cardiomyocytes. Zhongguo Yao Li Xue Bao. 1998;19(5):481-5. PubMed PMID: 10375816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristics of transient outward K+ current in human atrial cardiomyocytes. AU - Xu,W H, AU - Li,W, AU - Yue,H W, AU - Wang,X L, PY - 1999/6/22/pubmed PY - 1999/6/22/medline PY - 1999/6/22/entrez SP - 481 EP - 5 JF - Zhongguo yao li xue bao = Acta pharmacologica Sinica JO - Zhongguo Yao Li Xue Bao VL - 19 IS - 5 N2 - AIM: To study the properties of transient outward potassium current (Ito) in human atrial myocytes. METHODS: The patch-clamp whole-cell recording techniques were used. When the calcium inward current was blocked by CdCl2 (0.1 mmol.L-1), the transient outward potassium current (Ito) was recorded by depolarizing cells. RESULTS: The current was voltage dependent, it was activated quickly and inactivated rapidly too. 4-Aminopyridine (4-AP) 10 mmol.L-1 (a selective blocker of Ito) blocked the current completely. The IC50 (95% confidence limits) of 4-AP on Ito were 0.67 (0.58-0.80) mmol.L-1, 4-AP 1 mmol.L-1 shifted the activation curve of Ito to positive potential, therefore a higher membrane potential was required to activate Ito. CONCLUSION: Ito, a major K+ channel current in human atrial cells, is blocked by 4-AP. SN - 0253-9756 UR - https://www.unboundmedicine.com/medline/citation/10375816/Characteristics_of_transient_outward_K+_current_in_human_atrial_cardiomyocytes_ L2 - http://www.chinaphar.com/1671-4083/19/481.pdf DB - PRIME DP - Unbound Medicine ER -