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[Lipid degradation by way of beta and alpha oxidation in peroxisomes of mammals].
Verh K Acad Geneeskd Belg. 1999; 61(1):65-89.VK

Abstract

Recently, it has become clear that the peroxisomal beta-oxidation system in rat and man consists of multiple pathways. In rat and man straight chain fatty acids, dicarboxylic fatty acids and prostaglandins are oxidized via the L-specific pathway catalyzed by palmitoyl-CoA oxidase, multifunctional protein-1 and thiolase. 2-Methyl-branched fatty acids and the bile acid intermediates are oxidized via the D-specific pathway. In the rat this pathway is catalyzed by prostanoyl-CoA oxidase, multifunctional protein-2 and sterol carrier protein-X (branched fatty acids) and by trihydroxycoprostanoyl-CoA oxidase, multifunctional protein-2 and sterol carrier protein-X (bile acid intermediates). In the human, branched fatty acids and bile acid intermediates are oxidized via branched chain acyl-CoA oxidase, multifunctional protein-2 and sterol carrier protein-X. All enzymes of these pathways have been purified, cloned and characterized. Also the reactions that constitute the alpha-oxidation pathway for 3-methyl-branched fatty acids, have recently been identified in rat and man. The revised pathway consists of the following reactions: 1) an activation reaction catalyzed by an acyl-CoA synthetase, that forms a 3-methylacyl-CoA; 2) a hydroxylation (dioxygenase) reaction catalyzed by a 3-methylacyl-CoA 2-hydroxylase, that converts the CoA ester to a 2-hydroxy-3-methylacyl-CoA; 3) a cleavage reaction catalyzed by a 2-hydroxy-3-methylacyl-CoA lyase, that releases a 2-methyl fatty aldehyde and formyl-CoA. The branched aldehyde is dehydrogenated by an aldehyde dehydrogenase to a 2-methyl branched fatty acid that can be degraded by peroxisomal beta-oxidation. Formyl-CoA is enzymatically hydrolyzed to formate which is then converted to CO2.

Authors+Show Affiliations

Departement Moleculaire Celbiologie, Faculteit Geneeskunde-K.U.Leuven.

Pub Type(s)

English Abstract
Journal Article

Language

dut

PubMed ID

10379198

Citation

Mannaerts, G P.. "[Lipid Degradation By Way of Beta and Alpha Oxidation in Peroxisomes of Mammals]." Verhandelingen - Koninklijke Academie Voor Geneeskunde Van Belgie, vol. 61, no. 1, 1999, pp. 65-89.
Mannaerts GP. [Lipid degradation by way of beta and alpha oxidation in peroxisomes of mammals]. Verh K Acad Geneeskd Belg. 1999;61(1):65-89.
Mannaerts, G. P. (1999). [Lipid degradation by way of beta and alpha oxidation in peroxisomes of mammals]. Verhandelingen - Koninklijke Academie Voor Geneeskunde Van Belgie, 61(1), 65-89.
Mannaerts GP. [Lipid Degradation By Way of Beta and Alpha Oxidation in Peroxisomes of Mammals]. Verh K Acad Geneeskd Belg. 1999;61(1):65-89. PubMed PMID: 10379198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Lipid degradation by way of beta and alpha oxidation in peroxisomes of mammals]. A1 - Mannaerts,G P, PY - 1999/6/24/pubmed PY - 1999/6/24/medline PY - 1999/6/24/entrez SP - 65 EP - 89 JF - Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie JO - Verh K Acad Geneeskd Belg VL - 61 IS - 1 N2 - Recently, it has become clear that the peroxisomal beta-oxidation system in rat and man consists of multiple pathways. In rat and man straight chain fatty acids, dicarboxylic fatty acids and prostaglandins are oxidized via the L-specific pathway catalyzed by palmitoyl-CoA oxidase, multifunctional protein-1 and thiolase. 2-Methyl-branched fatty acids and the bile acid intermediates are oxidized via the D-specific pathway. In the rat this pathway is catalyzed by prostanoyl-CoA oxidase, multifunctional protein-2 and sterol carrier protein-X (branched fatty acids) and by trihydroxycoprostanoyl-CoA oxidase, multifunctional protein-2 and sterol carrier protein-X (bile acid intermediates). In the human, branched fatty acids and bile acid intermediates are oxidized via branched chain acyl-CoA oxidase, multifunctional protein-2 and sterol carrier protein-X. All enzymes of these pathways have been purified, cloned and characterized. Also the reactions that constitute the alpha-oxidation pathway for 3-methyl-branched fatty acids, have recently been identified in rat and man. The revised pathway consists of the following reactions: 1) an activation reaction catalyzed by an acyl-CoA synthetase, that forms a 3-methylacyl-CoA; 2) a hydroxylation (dioxygenase) reaction catalyzed by a 3-methylacyl-CoA 2-hydroxylase, that converts the CoA ester to a 2-hydroxy-3-methylacyl-CoA; 3) a cleavage reaction catalyzed by a 2-hydroxy-3-methylacyl-CoA lyase, that releases a 2-methyl fatty aldehyde and formyl-CoA. The branched aldehyde is dehydrogenated by an aldehyde dehydrogenase to a 2-methyl branched fatty acid that can be degraded by peroxisomal beta-oxidation. Formyl-CoA is enzymatically hydrolyzed to formate which is then converted to CO2. SN - 0302-6469 UR - https://www.unboundmedicine.com/medline/citation/10379198/[Lipid_degradation_by_way_of_beta_and_alpha_oxidation_in_peroxisomes_of_mammals]_ L2 - https://antibodies.cancer.gov/detail/CPTC-OTUB1-1 DB - PRIME DP - Unbound Medicine ER -
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