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Neuronal, glial, and epithelial localization of gamma-aminobutyric acid transporter 2, a high-affinity gamma-aminobutyric acid plasma membrane transporter, in the cerebral cortex and neighboring structures.
J Comp Neurol. 1999 Jul 05; 409(3):482-94.JC

Abstract

Neuronal and glial high-affinity Na+/Cl(-)-dependent plasma membrane gamma-aminobutyric acid (GABA) transporters (GATs) contribute to regulating neuronal function. We investigated in the cerebral cortex and neighboring regions of adult rats the distribution and cellular localization of the GABA transporter GAT-2 by immunocytochemistry with affinity-purified polyclonal antibodies that react monospecifically with a protein of 82 kDa. Conventional and confocal laser-scanning light microscopic studies revealed intense GAT-2 immunoreactivity (ir) in the leptomeninges, choroid plexus, and ependyma. Weak GAT-2 immunoreactivity also was observed in the cortical parenchyma, where it was localized to puncta of different sizes scattered throughout the radial extension of the neocortex and to few cell bodies. In sections double-labeled with GAT-2 and glial fibrillary acidic protein (GFAP) antibodies, some GAT-2-positive profiles also were GFAP positive. Ultrastructural studies showed GAT-2 immunoreactivity mostly in patches of varying sizes scattered in the cytoplasm of neuronal and nonneuronal elements: GAT-2-positive neuronal elements included perikarya, dendrites, and axon terminals forming both symmetric and asymmetric synapses; nonneuronal elements expressing GAT-2 were cells forming the pia and arachnoid mater; astrocytic processes, including glia limitans and perivascular end feet; ependymal cells; and epithelial cells of the choroid plexuses. The widespread cellular expression of GAT-2 suggests that it may have several functional roles in the overall regulation of GABA levels in the brain.

Authors+Show Affiliations

Institute of Human Physiology, University of Ancona, Italy. f.conti@popcsi.unian.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10379832

Citation

Conti, F, et al. "Neuronal, Glial, and Epithelial Localization of Gamma-aminobutyric Acid Transporter 2, a High-affinity Gamma-aminobutyric Acid Plasma Membrane Transporter, in the Cerebral Cortex and Neighboring Structures." The Journal of Comparative Neurology, vol. 409, no. 3, 1999, pp. 482-94.
Conti F, Zuccarello LV, Barbaresi P, et al. Neuronal, glial, and epithelial localization of gamma-aminobutyric acid transporter 2, a high-affinity gamma-aminobutyric acid plasma membrane transporter, in the cerebral cortex and neighboring structures. J Comp Neurol. 1999;409(3):482-94.
Conti, F., Zuccarello, L. V., Barbaresi, P., Minelli, A., Brecha, N. C., & Melone, M. (1999). Neuronal, glial, and epithelial localization of gamma-aminobutyric acid transporter 2, a high-affinity gamma-aminobutyric acid plasma membrane transporter, in the cerebral cortex and neighboring structures. The Journal of Comparative Neurology, 409(3), 482-94.
Conti F, et al. Neuronal, Glial, and Epithelial Localization of Gamma-aminobutyric Acid Transporter 2, a High-affinity Gamma-aminobutyric Acid Plasma Membrane Transporter, in the Cerebral Cortex and Neighboring Structures. J Comp Neurol. 1999 Jul 5;409(3):482-94. PubMed PMID: 10379832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal, glial, and epithelial localization of gamma-aminobutyric acid transporter 2, a high-affinity gamma-aminobutyric acid plasma membrane transporter, in the cerebral cortex and neighboring structures. AU - Conti,F, AU - Zuccarello,L V, AU - Barbaresi,P, AU - Minelli,A, AU - Brecha,N C, AU - Melone,M, PY - 1999/6/24/pubmed PY - 2000/6/20/medline PY - 1999/6/24/entrez SP - 482 EP - 94 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 409 IS - 3 N2 - Neuronal and glial high-affinity Na+/Cl(-)-dependent plasma membrane gamma-aminobutyric acid (GABA) transporters (GATs) contribute to regulating neuronal function. We investigated in the cerebral cortex and neighboring regions of adult rats the distribution and cellular localization of the GABA transporter GAT-2 by immunocytochemistry with affinity-purified polyclonal antibodies that react monospecifically with a protein of 82 kDa. Conventional and confocal laser-scanning light microscopic studies revealed intense GAT-2 immunoreactivity (ir) in the leptomeninges, choroid plexus, and ependyma. Weak GAT-2 immunoreactivity also was observed in the cortical parenchyma, where it was localized to puncta of different sizes scattered throughout the radial extension of the neocortex and to few cell bodies. In sections double-labeled with GAT-2 and glial fibrillary acidic protein (GFAP) antibodies, some GAT-2-positive profiles also were GFAP positive. Ultrastructural studies showed GAT-2 immunoreactivity mostly in patches of varying sizes scattered in the cytoplasm of neuronal and nonneuronal elements: GAT-2-positive neuronal elements included perikarya, dendrites, and axon terminals forming both symmetric and asymmetric synapses; nonneuronal elements expressing GAT-2 were cells forming the pia and arachnoid mater; astrocytic processes, including glia limitans and perivascular end feet; ependymal cells; and epithelial cells of the choroid plexuses. The widespread cellular expression of GAT-2 suggests that it may have several functional roles in the overall regulation of GABA levels in the brain. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/10379832/Neuronal_glial_and_epithelial_localization_of_gamma_aminobutyric_acid_transporter_2_a_high_affinity_gamma_aminobutyric_acid_plasma_membrane_transporter_in_the_cerebral_cortex_and_neighboring_structures_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1999&volume=409&issue=3&spage=482 DB - PRIME DP - Unbound Medicine ER -