Tags

Type your tag names separated by a space and hit enter

Cellular immune response of adenoidal and tonsillar lymphocytes to the P6 outer membrane protein of non-typeable Haemophilus influenzae and its relation to otitis media.
Acta Otolaryngol. 1999; 119(3):377-83.AO

Abstract

Cellular immune responses to the P6 outer membrane protein of non-typeable Haemophilus influenzae (NTHi) were determined in vitro by measuring immunoglobulin (Ig) secreting cells and lymphocyte proliferation in adenoidal and tonsillar lymphocytes from 19 children. Preliminary tests showed that P6 did not stimulate naive cells such as cord blood lymphocytes, but did stimulate sensitized cells in adenoids and tonsils. Cellular proliferation was significantly higher in adenoidal lymphocytes than in tonsillar lymphocytes (median: quadratile of stimulation index = 3.7:2.3-5.5 vs. 1.2:1.0-2.1, p < 0.02). A comparison between children with or without otitis media revealed that proliferative responses to P6 of adenoidal lymphocytes from children with otitis media were significantly decreased (2.0:1.8-3.6 vs. 3.7:2.3-5.5, p < 0.04). P6-specific antibody secreting cells were identified in a total of 14 adenoids and the number of cells secreting IgA was decreased in the otitis media group compared to controls (median: quadratile/10(6) cells = 435:359-499 vs. 755:593-1870, p < 0.05). Cultivation with P6 stimulated IgA secretion in children without otitis media, while no response was seen in children with otitis media (median: quadratile/10(6) cells = 1323:915-2410 vs. 2240:1900-2830, p < 0.02). These preliminary data demonstrate that lymphocytes from adenoids and tonsils recognize P6 as a specific antigen and that the adenoid is the more reactive of the two organs. Impaired P6-specific cellular immune responses of adenoids in children with otitis media may explain the recurrent nature of otitis media due to NTHi in the otitis prone population.

Links

Publisher Full Text

Authors+Show Affiliations

Kodama H
Department of Otolaryngology, Sapporo Medical University, Japan.
Faden H
No affiliation info available
Harabuchi Y
No affiliation info available
Kataura A
No affiliation info available
Bernstein JM
No affiliation info available
Brodsky L
No affiliation info available

MeSH

Acute DiseaseAdenoidsAntibodies, BacterialAntibody SpecificityBacterial Outer Membrane ProteinsCell MovementChildChild, PreschoolFemaleHaemophilus InfectionsHaemophilus VaccinesHumansMaleNasopharynxOtitis Media with EffusionPalatine TonsilT-Lymphocytes

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10380746

Citation

Kodama, H, et al. "Cellular Immune Response of Adenoidal and Tonsillar Lymphocytes to the P6 Outer Membrane Protein of Non-typeable Haemophilus Influenzae and Its Relation to Otitis Media." Acta Oto-laryngologica, vol. 119, no. 3, 1999, pp. 377-83.
Kodama H, Faden H, Harabuchi Y, et al. Cellular immune response of adenoidal and tonsillar lymphocytes to the P6 outer membrane protein of non-typeable Haemophilus influenzae and its relation to otitis media. Acta Otolaryngol. 1999;119(3):377-83.
Kodama, H., Faden, H., Harabuchi, Y., Kataura, A., Bernstein, J. M., & Brodsky, L. (1999). Cellular immune response of adenoidal and tonsillar lymphocytes to the P6 outer membrane protein of non-typeable Haemophilus influenzae and its relation to otitis media. Acta Oto-laryngologica, 119(3), 377-83.
Kodama H, et al. Cellular Immune Response of Adenoidal and Tonsillar Lymphocytes to the P6 Outer Membrane Protein of Non-typeable Haemophilus Influenzae and Its Relation to Otitis Media. Acta Otolaryngol. 1999;119(3):377-83. PubMed PMID: 10380746.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular immune response of adenoidal and tonsillar lymphocytes to the P6 outer membrane protein of non-typeable Haemophilus influenzae and its relation to otitis media. AU - Kodama,H, AU - Faden,H, AU - Harabuchi,Y, AU - Kataura,A, AU - Bernstein,J M, AU - Brodsky,L, PY - 1999/6/25/pubmed PY - 1999/6/25/medline PY - 1999/6/25/entrez SP - 377 EP - 83 JF - Acta oto-laryngologica JO - Acta Otolaryngol VL - 119 IS - 3 N2 - Cellular immune responses to the P6 outer membrane protein of non-typeable Haemophilus influenzae (NTHi) were determined in vitro by measuring immunoglobulin (Ig) secreting cells and lymphocyte proliferation in adenoidal and tonsillar lymphocytes from 19 children. Preliminary tests showed that P6 did not stimulate naive cells such as cord blood lymphocytes, but did stimulate sensitized cells in adenoids and tonsils. Cellular proliferation was significantly higher in adenoidal lymphocytes than in tonsillar lymphocytes (median: quadratile of stimulation index = 3.7:2.3-5.5 vs. 1.2:1.0-2.1, p < 0.02). A comparison between children with or without otitis media revealed that proliferative responses to P6 of adenoidal lymphocytes from children with otitis media were significantly decreased (2.0:1.8-3.6 vs. 3.7:2.3-5.5, p < 0.04). P6-specific antibody secreting cells were identified in a total of 14 adenoids and the number of cells secreting IgA was decreased in the otitis media group compared to controls (median: quadratile/10(6) cells = 435:359-499 vs. 755:593-1870, p < 0.05). Cultivation with P6 stimulated IgA secretion in children without otitis media, while no response was seen in children with otitis media (median: quadratile/10(6) cells = 1323:915-2410 vs. 2240:1900-2830, p < 0.02). These preliminary data demonstrate that lymphocytes from adenoids and tonsils recognize P6 as a specific antigen and that the adenoid is the more reactive of the two organs. Impaired P6-specific cellular immune responses of adenoids in children with otitis media may explain the recurrent nature of otitis media due to NTHi in the otitis prone population. SN - 0001-6489 UR - https://www.unboundmedicine.com/medline/citation/10380746/Cellular_immune_response_of_adenoidal_and_tonsillar_lymphocytes_to_the_P6_outer_membrane_protein_of_non_typeable_Haemophilus_influenzae_and_its_relation_to_otitis_media_ L2 - https://www.tandfonline.com/doi/full/10.1080/00016489950181422 DB - PRIME DP - Unbound Medicine ER -