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The major opsin in bees (Insecta: Hymenoptera): A promising nuclear gene for higher level phylogenetics.
Mol Phylogenet Evol. 1999 Jul; 12(2):168-76.MP

Abstract

We report the phylogenetic utility of the nuclear gene encoding the long-wavelength opsin (LW Rh) for tribes of bees. Aligned nucleotide sequences were examined in multiple taxa from the four tribes comprising the corbiculate bees within the subfamily Apinae. Phylogenetic analyses of sequence variation in a 502-bp fragment (approx 40% of the coding region) strongly supported the monophyly of each of the four tribes, which are well established from previous studies of morphology and DNA. Trees estimated from parsimony and maximum likelihood analyses of LW Rh sequences show a strongly supported relationship between the tribes Meliponini and Bombini, a relationship that has been found uniformly in studies of other genes (28S, 16S, and cytochrome b). All of the tribal clades as well as relationships among the tribes are supported by high bootstrap values, suggesting the utility of LW Rh in estimating tribal and subfamily rank for these bees. The sequences exhibit minimal base composition bias. Both 1st + 2nd and 3rd position sites provide information for estimating a reliable tree topology. These results suggest that LW Rh, which has not been reported previously in studies of organismal phylogenetics, could provide important new data from the nuclear genome for phylogeny reconstruction.

Authors+Show Affiliations

Department of Entomology, University of Arkansas, Fayetteville, Arkansas 72701, USA.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

10381319

Citation

Mardulyn, P, and S A. Cameron. "The Major Opsin in Bees (Insecta: Hymenoptera): a Promising Nuclear Gene for Higher Level Phylogenetics." Molecular Phylogenetics and Evolution, vol. 12, no. 2, 1999, pp. 168-76.
Mardulyn P, Cameron SA. The major opsin in bees (Insecta: Hymenoptera): A promising nuclear gene for higher level phylogenetics. Mol Phylogenet Evol. 1999;12(2):168-76.
Mardulyn, P., & Cameron, S. A. (1999). The major opsin in bees (Insecta: Hymenoptera): A promising nuclear gene for higher level phylogenetics. Molecular Phylogenetics and Evolution, 12(2), 168-76.
Mardulyn P, Cameron SA. The Major Opsin in Bees (Insecta: Hymenoptera): a Promising Nuclear Gene for Higher Level Phylogenetics. Mol Phylogenet Evol. 1999;12(2):168-76. PubMed PMID: 10381319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The major opsin in bees (Insecta: Hymenoptera): A promising nuclear gene for higher level phylogenetics. AU - Mardulyn,P, AU - Cameron,S A, PY - 1999/6/25/pubmed PY - 1999/6/25/medline PY - 1999/6/25/entrez SP - 168 EP - 76 JF - Molecular phylogenetics and evolution JO - Mol Phylogenet Evol VL - 12 IS - 2 N2 - We report the phylogenetic utility of the nuclear gene encoding the long-wavelength opsin (LW Rh) for tribes of bees. Aligned nucleotide sequences were examined in multiple taxa from the four tribes comprising the corbiculate bees within the subfamily Apinae. Phylogenetic analyses of sequence variation in a 502-bp fragment (approx 40% of the coding region) strongly supported the monophyly of each of the four tribes, which are well established from previous studies of morphology and DNA. Trees estimated from parsimony and maximum likelihood analyses of LW Rh sequences show a strongly supported relationship between the tribes Meliponini and Bombini, a relationship that has been found uniformly in studies of other genes (28S, 16S, and cytochrome b). All of the tribal clades as well as relationships among the tribes are supported by high bootstrap values, suggesting the utility of LW Rh in estimating tribal and subfamily rank for these bees. The sequences exhibit minimal base composition bias. Both 1st + 2nd and 3rd position sites provide information for estimating a reliable tree topology. These results suggest that LW Rh, which has not been reported previously in studies of organismal phylogenetics, could provide important new data from the nuclear genome for phylogeny reconstruction. SN - 1055-7903 UR - https://www.unboundmedicine.com/medline/citation/10381319/The_major_opsin_in_bees__Insecta:_Hymenoptera_:_A_promising_nuclear_gene_for_higher_level_phylogenetics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1055-7903(98)90606-6 DB - PRIME DP - Unbound Medicine ER -