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Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway.
J Cell Biol. 1999 Jun 28; 145(7):1369-80.JC

Abstract

A collection of yeast strains surviving with mutant 5S RNA has been constructed. The mutant strains presented alterations of the nucleolar structure, with less granular component, and a delocalization of the 25S rRNA throughout the nucleoplasm. The 5S RNA mutations affected helix I and resulted in decreased amounts of stable 5S RNA and of the ribosomal 60S subunits. The shortage of 60S subunits was due to a specific defect in the processing of the 27SB precursor RNA that gives rise to the mature 25S and 5.8S rRNA. The processing rate of the 27SB pre-rRNA was specifically delayed, whereas the 27SA and 20S pre-rRNA were processed at a normal rate. The defect was partially corrected by increasing the amount of mutant 5S RNA. We propose that the 5S RNA is recruited by the pre-60S particle and that its recruitment is necessary for the efficient processing of the 27SB RNA precursor. Such a mechanism could ensure that all newly formed mature 60S subunits contain stoichiometric amounts of the three rRNA components.

Authors+Show Affiliations

Service de Biochimie et de Génétique Moléculaire, Commissariat á L'Energie Atomique (CEA)/Saclay, F-91191 Gif-sur-Yvette Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10385518

Citation

Dechampesme, A M., et al. "Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the pre-rRNA Processing Pathway." The Journal of Cell Biology, vol. 145, no. 7, 1999, pp. 1369-80.
Dechampesme AM, Koroleva O, Leger-Silvestre I, et al. Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway. J Cell Biol. 1999;145(7):1369-80.
Dechampesme, A. M., Koroleva, O., Leger-Silvestre, I., Gas, N., & Camier, S. (1999). Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway. The Journal of Cell Biology, 145(7), 1369-80.
Dechampesme AM, et al. Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the pre-rRNA Processing Pathway. J Cell Biol. 1999 Jun 28;145(7):1369-80. PubMed PMID: 10385518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway. AU - Dechampesme,A M, AU - Koroleva,O, AU - Leger-Silvestre,I, AU - Gas,N, AU - Camier,S, PY - 1999/6/29/pubmed PY - 1999/6/29/medline PY - 1999/6/29/entrez SP - 1369 EP - 80 JF - The Journal of cell biology JO - J Cell Biol VL - 145 IS - 7 N2 - A collection of yeast strains surviving with mutant 5S RNA has been constructed. The mutant strains presented alterations of the nucleolar structure, with less granular component, and a delocalization of the 25S rRNA throughout the nucleoplasm. The 5S RNA mutations affected helix I and resulted in decreased amounts of stable 5S RNA and of the ribosomal 60S subunits. The shortage of 60S subunits was due to a specific defect in the processing of the 27SB precursor RNA that gives rise to the mature 25S and 5.8S rRNA. The processing rate of the 27SB pre-rRNA was specifically delayed, whereas the 27SA and 20S pre-rRNA were processed at a normal rate. The defect was partially corrected by increasing the amount of mutant 5S RNA. We propose that the 5S RNA is recruited by the pre-60S particle and that its recruitment is necessary for the efficient processing of the 27SB RNA precursor. Such a mechanism could ensure that all newly formed mature 60S subunits contain stoichiometric amounts of the three rRNA components. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/10385518/Assembly_of_5S_ribosomal_RNA_is_required_at_a_specific_step_of_the_pre_rRNA_processing_pathway_ L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.145.7.1369 DB - PRIME DP - Unbound Medicine ER -