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Structural determination of the acidic exopolysaccharide produced by a Pseudomonas sp. strain 1.15.
Carbohydr Res. 1999 Jan 31; 315(1-2):159-68.CR

Abstract

Pseudomonas strain 1.15 was isolated from a freshwater biofilm and shown to produce considerable amounts of an acidic polysaccharide which was investigated by methylation analysis, NMR spectroscopy and ionspray mass spectrometry (ISMS). The polysaccharide was depolymerised by a bacteriophage-associated endoglucosidase and by autohydrolysis, and the resulting oligosaccharides were investigated by NMR spectroscopy and mass spectrometry. The resulting data showed that the parent repeating unit of the 1.15 exopolysaccharide (EPS) is a branched hexasaccharide. The main chain is constituted of the trisaccharide -->4)-alpha-L-Fucp-(1-->4)-alpha-L-Fucp-(1-->3)-beta-D-Glcp- (1--> and the side chain alpha-D-Galp-(1-->4)-beta-D-GlcAp-(1-->3)-alpha-D-Galp-(1-->is linked to O-3 of the first Fuc residue. The terminal non-reducing Gal carries a 1-carboxyethylidene acetal in the R configuration at the positions 4 and 6. Of the four different O-acetyl groups present in non-stoichiometric amounts, two were established to be on O-2 of the 3-linked Gal and on O-2 of the 4-linked Fuc.

Authors+Show Affiliations

Cescutti P
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, Italy. cescutti@bbcm.univ.trieste.it
Toffanin R
No affiliation info available
Pollesello P
No affiliation info available
Sutherland IW
No affiliation info available

MeSH

Carbohydrate SequenceMagnetic Resonance SpectroscopyMass SpectrometryMolecular Sequence DataMolecular StructurePolysaccharides, BacterialPseudomonas

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10385979

Citation

Cescutti, P, et al. "Structural Determination of the Acidic Exopolysaccharide Produced By a Pseudomonas Sp. Strain 1.15." Carbohydrate Research, vol. 315, no. 1-2, 1999, pp. 159-68.
Cescutti P, Toffanin R, Pollesello P, et al. Structural determination of the acidic exopolysaccharide produced by a Pseudomonas sp. strain 1.15. Carbohydr Res. 1999;315(1-2):159-68.
Cescutti, P., Toffanin, R., Pollesello, P., & Sutherland, I. W. (1999). Structural determination of the acidic exopolysaccharide produced by a Pseudomonas sp. strain 1.15. Carbohydrate Research, 315(1-2), 159-68.
Cescutti P, et al. Structural Determination of the Acidic Exopolysaccharide Produced By a Pseudomonas Sp. Strain 1.15. Carbohydr Res. 1999 Jan 31;315(1-2):159-68. PubMed PMID: 10385979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural determination of the acidic exopolysaccharide produced by a Pseudomonas sp. strain 1.15. AU - Cescutti,P, AU - Toffanin,R, AU - Pollesello,P, AU - Sutherland,I W, PY - 1999/7/1/pubmed PY - 1999/7/1/medline PY - 1999/7/1/entrez SP - 159 EP - 68 JF - Carbohydrate research JO - Carbohydr Res VL - 315 IS - 1-2 N2 - Pseudomonas strain 1.15 was isolated from a freshwater biofilm and shown to produce considerable amounts of an acidic polysaccharide which was investigated by methylation analysis, NMR spectroscopy and ionspray mass spectrometry (ISMS). The polysaccharide was depolymerised by a bacteriophage-associated endoglucosidase and by autohydrolysis, and the resulting oligosaccharides were investigated by NMR spectroscopy and mass spectrometry. The resulting data showed that the parent repeating unit of the 1.15 exopolysaccharide (EPS) is a branched hexasaccharide. The main chain is constituted of the trisaccharide -->4)-alpha-L-Fucp-(1-->4)-alpha-L-Fucp-(1-->3)-beta-D-Glcp- (1--> and the side chain alpha-D-Galp-(1-->4)-beta-D-GlcAp-(1-->3)-alpha-D-Galp-(1-->is linked to O-3 of the first Fuc residue. The terminal non-reducing Gal carries a 1-carboxyethylidene acetal in the R configuration at the positions 4 and 6. Of the four different O-acetyl groups present in non-stoichiometric amounts, two were established to be on O-2 of the 3-linked Gal and on O-2 of the 4-linked Fuc. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/10385979/Structural_determination_of_the_acidic_exopolysaccharide_produced_by_a_Pseudomonas_sp__strain_1_15_ DB - PRIME DP - Unbound Medicine ER -