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HLA-B27 subtypes and HLA class II alleles in Japanese patients with anterior uveitis.
Invest Ophthalmol Vis Sci. 1999 Jul; 40(8):1838-44.IO

Abstract

PURPOSE

Some patients with anterior uveitis (AU) have ankylosing spondylitis (AS) and are HLA-B27 class I-positive. The purpose of this study was to investigate whether there are differences in HLA at the allele level among each group of patients with AU.

METHODS

Seventy-three patients with AU were studied. They were classified into three groups: 31 with AS-associated AU, 14 with HLA-B27-associated AU, and 28 with idiopathic AU. Three control groups without AU were used: 138 random subjects, 33 HLA-B27-positive healthy subjects, and 19 HLA-B27-positive patients with AS. DRB1 and DQB1 genotyping was performed using polymerase chain reaction (PCR)-single-strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism. HLA-B27 subtype was determined by PCR-SSCP.

RESULTS

There was no difference in the frequency of any class I antigen except HLA-B27 among the patients studied. The frequencies of HLA-DR12 in AS-associated AU and HLA-DR1 in HLA-B27-associated AU showed an increase. In HLA-B27-associated AU, DRB1*0101 and DQB1*0501 were increased compared with HLA-B27-positive control subjects. When HLA-B27 subtype distribution was compared among the groups, the proportion of B*2704 was significantly lower in HLA-B27-associated AU (P = 0.037), however, such a difference was not present in AS-associated groups.

CONCLUSIONS

These results indicated that B*2704 seemed to be less susceptible to AU compared with B*2705 in Japanese subjects. The increase of HLA-DR12 and HLA-DR1 in AU may be caused by linkage disequilibrium with B*2704 and B*2705, respectively.

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Authors+Show Affiliations

Konno Y
Department of Ophthalmology, Faculty of Medicine, University of Tokyo, Japan.
Numaga J
No affiliation info available
Tsuchiya N
No affiliation info available
Ogawa A
No affiliation info available
Islam SM
No affiliation info available
Mochizuki M
No affiliation info available
Mitsui H
No affiliation info available
Oda H
No affiliation info available
Maeda H
No affiliation info available

MeSH

AdultAllelesDNADNA PrimersFemaleGenotypeHLA-B27 AntigenHistocompatibility Antigens Class IIHumansJapanMalePolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPolymorphism, Single-Stranded ConformationalSpondylitis, AnkylosingUveitis, Anterior

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10393058

Citation

Konno, Y, et al. "HLA-B27 Subtypes and HLA Class II Alleles in Japanese Patients With Anterior Uveitis." Investigative Ophthalmology & Visual Science, vol. 40, no. 8, 1999, pp. 1838-44.
Konno Y, Numaga J, Tsuchiya N, et al. HLA-B27 subtypes and HLA class II alleles in Japanese patients with anterior uveitis. Invest Ophthalmol Vis Sci. 1999;40(8):1838-44.
Konno, Y., Numaga, J., Tsuchiya, N., Ogawa, A., Islam, S. M., Mochizuki, M., Mitsui, H., Oda, H., & Maeda, H. (1999). HLA-B27 subtypes and HLA class II alleles in Japanese patients with anterior uveitis. Investigative Ophthalmology & Visual Science, 40(8), 1838-44.
Konno Y, et al. HLA-B27 Subtypes and HLA Class II Alleles in Japanese Patients With Anterior Uveitis. Invest Ophthalmol Vis Sci. 1999;40(8):1838-44. PubMed PMID: 10393058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HLA-B27 subtypes and HLA class II alleles in Japanese patients with anterior uveitis. AU - Konno,Y, AU - Numaga,J, AU - Tsuchiya,N, AU - Ogawa,A, AU - Islam,S M, AU - Mochizuki,M, AU - Mitsui,H, AU - Oda,H, AU - Maeda,H, PY - 1999/7/7/pubmed PY - 1999/7/7/medline PY - 1999/7/7/entrez SP - 1838 EP - 44 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 40 IS - 8 N2 - PURPOSE: Some patients with anterior uveitis (AU) have ankylosing spondylitis (AS) and are HLA-B27 class I-positive. The purpose of this study was to investigate whether there are differences in HLA at the allele level among each group of patients with AU. METHODS: Seventy-three patients with AU were studied. They were classified into three groups: 31 with AS-associated AU, 14 with HLA-B27-associated AU, and 28 with idiopathic AU. Three control groups without AU were used: 138 random subjects, 33 HLA-B27-positive healthy subjects, and 19 HLA-B27-positive patients with AS. DRB1 and DQB1 genotyping was performed using polymerase chain reaction (PCR)-single-strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism. HLA-B27 subtype was determined by PCR-SSCP. RESULTS: There was no difference in the frequency of any class I antigen except HLA-B27 among the patients studied. The frequencies of HLA-DR12 in AS-associated AU and HLA-DR1 in HLA-B27-associated AU showed an increase. In HLA-B27-associated AU, DRB1*0101 and DQB1*0501 were increased compared with HLA-B27-positive control subjects. When HLA-B27 subtype distribution was compared among the groups, the proportion of B*2704 was significantly lower in HLA-B27-associated AU (P = 0.037), however, such a difference was not present in AS-associated groups. CONCLUSIONS: These results indicated that B*2704 seemed to be less susceptible to AU compared with B*2705 in Japanese subjects. The increase of HLA-DR12 and HLA-DR1 in AU may be caused by linkage disequilibrium with B*2704 and B*2705, respectively. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/10393058/HLA_B27_subtypes_and_HLA_class_II_alleles_in_Japanese_patients_with_anterior_uveitis_ L2 - https://iovs.arvojournals.org/article.aspx?volume=40&issue=8&page=1838 DB - PRIME DP - Unbound Medicine ER -