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[The importance of polymerase chain reaction in the diagnosis of enterovirus infections of the central nervous system in children. Clinico-epidemiologic characteristics].
Enferm Infecc Microbiol Clin. 1999 May; 17(5):227-30.EI

Abstract

BACKGROUND

To compare the diagnosis utility of a reverse transcription-polymerase chain reaction (RT-PCR) for detection of enteroviral RNA in cerebrospinal fluid (CSF) in comparison to viral culture for central nervous system infections in pediatric population and to know the clinic epidemiological characteristics of this infection.

METHODS

From June to December of 1997, 116 CSF samples of children were included in the study. The samples were inoculated in MRC-5 and HEP-2 and the enterovirus RNA was detected with AMPLICOR-Enterovirus (Roche Diagnostic System). The virus were typed by neutralization.

RESULTS

36 samples were positive (30 were positive for RT-PCR and 6 for RT-PCR and viral culture). The media time in detect the CPE was 4.8 days. The viruses found were four echovirus 6, one echovirus 30 and one echovirus 7. Fifteen children were under 6 months (93.3% under two months) and 21 children over 6 months with a media age of 81 months (range, 38-160 months). In over 6 months old children, the most frequent clinical presentation was meningitis aseptic syndrome with pleocytosis and in under 6 months old ones was fever and only 60% of these children had pleocytosis. The evolution was good in all the cases.

CONCLUSIONS

AMPLICOR-Enterovirus is a sensitive method for the diagnosis of enteroviral meningitis. The clinical manifestations are different with the age and due to the absence of neurological symptoms and pleocytosis in the patients under two months, we think that it is necessary to study the presence of enterovirus with genetic amplification methods in this population of patients.

Authors+Show Affiliations

Servicio de Microbiología, Hospital Nuestra Señora de Pino, Las Palmas de Gran Canaria. mjpena@correo.hpino.rcanaria.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

spa

PubMed ID

10396087

Citation

Pena, M J., et al. "[The Importance of Polymerase Chain Reaction in the Diagnosis of Enterovirus Infections of the Central Nervous System in Children. Clinico-epidemiologic Characteristics]." Enfermedades Infecciosas Y Microbiologia Clinica, vol. 17, no. 5, 1999, pp. 227-30.
Pena MJ, Bolaños M, Pérez MC, et al. [The importance of polymerase chain reaction in the diagnosis of enterovirus infections of the central nervous system in children. Clinico-epidemiologic characteristics]. Enferm Infecc Microbiol Clin. 1999;17(5):227-30.
Pena, M. J., Bolaños, M., Pérez, M. C., Mosquera, M. M., Trallero, G., & Lafarga, B. (1999). [The importance of polymerase chain reaction in the diagnosis of enterovirus infections of the central nervous system in children. Clinico-epidemiologic characteristics]. Enfermedades Infecciosas Y Microbiologia Clinica, 17(5), 227-30.
Pena MJ, et al. [The Importance of Polymerase Chain Reaction in the Diagnosis of Enterovirus Infections of the Central Nervous System in Children. Clinico-epidemiologic Characteristics]. Enferm Infecc Microbiol Clin. 1999;17(5):227-30. PubMed PMID: 10396087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The importance of polymerase chain reaction in the diagnosis of enterovirus infections of the central nervous system in children. Clinico-epidemiologic characteristics]. AU - Pena,M J, AU - Bolaños,M, AU - Pérez,M C, AU - Mosquera,M M, AU - Trallero,G, AU - Lafarga,B, PY - 1999/7/9/pubmed PY - 1999/7/9/medline PY - 1999/7/9/entrez SP - 227 EP - 30 JF - Enfermedades infecciosas y microbiologia clinica JO - Enferm Infecc Microbiol Clin VL - 17 IS - 5 N2 - BACKGROUND: To compare the diagnosis utility of a reverse transcription-polymerase chain reaction (RT-PCR) for detection of enteroviral RNA in cerebrospinal fluid (CSF) in comparison to viral culture for central nervous system infections in pediatric population and to know the clinic epidemiological characteristics of this infection. METHODS: From June to December of 1997, 116 CSF samples of children were included in the study. The samples were inoculated in MRC-5 and HEP-2 and the enterovirus RNA was detected with AMPLICOR-Enterovirus (Roche Diagnostic System). The virus were typed by neutralization. RESULTS: 36 samples were positive (30 were positive for RT-PCR and 6 for RT-PCR and viral culture). The media time in detect the CPE was 4.8 days. The viruses found were four echovirus 6, one echovirus 30 and one echovirus 7. Fifteen children were under 6 months (93.3% under two months) and 21 children over 6 months with a media age of 81 months (range, 38-160 months). In over 6 months old children, the most frequent clinical presentation was meningitis aseptic syndrome with pleocytosis and in under 6 months old ones was fever and only 60% of these children had pleocytosis. The evolution was good in all the cases. CONCLUSIONS: AMPLICOR-Enterovirus is a sensitive method for the diagnosis of enteroviral meningitis. The clinical manifestations are different with the age and due to the absence of neurological symptoms and pleocytosis in the patients under two months, we think that it is necessary to study the presence of enterovirus with genetic amplification methods in this population of patients. SN - 0213-005X UR - https://www.unboundmedicine.com/medline/citation/10396087/[The_importance_of_polymerase_chain_reaction_in_the_diagnosis_of_enterovirus_infections_of_the_central_nervous_system_in_children__Clinico_epidemiologic_characteristics]_ DB - PRIME DP - Unbound Medicine ER -