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Enantioselective high-performance liquid chromatography with fluorescence detection of methamphetamine and its metabolites in human urine.
Analyst. 1998 Nov; 123(11):2333-7.A

Abstract

A simple, rapid and highly sensitive high-performance liquid chromatographic method with fluorescence detection for determining the enantiomers of methamphetamine and its major metabolites, amphetamine and p-hydroxymethamphetamine, in urine samples was developed. Using a newly developed reagent for amines, namely, 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride, six enantiomers were derivatized under mild conditions (i.e., 10 min at room temperature, pH 9.0) and separated isocratically on a cellulose tris(3,5-dimethylphenylcarbamate) coated silica gel column following a pre-separation on an ODS column within 42 min, and the effluent was monitored at 440 nm (lambda ex 330 nm). Calibration curves for these derivatives using spiked human urine were linear in the range 0.05-100 mumol dm-3 with correlation coefficients > or = 0.999. The detection limits at a signal-to-noise ratio of 3 were 2.8-8.8 fmol per 5 microliters injection. The relative standard deviations of within- (n = 6) and between-day (n = 5) variations were < or = 7.4%. The method was successfully applied to discriminate between (S)-(+)-methamphetamine and its corresponding metabolites found in abusers' urine and their antipodes in a sample taken from a Parkinsonian patient on selegiline (Deprenyl) therapy.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Nagasaki University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10396810

Citation

al-Dirbashi, O, et al. "Enantioselective High-performance Liquid Chromatography With Fluorescence Detection of Methamphetamine and Its Metabolites in Human Urine." The Analyst, vol. 123, no. 11, 1998, pp. 2333-7.
al-Dirbashi O, Kuroda N, Menichini F, et al. Enantioselective high-performance liquid chromatography with fluorescence detection of methamphetamine and its metabolites in human urine. Analyst. 1998;123(11):2333-7.
al-Dirbashi, O., Kuroda, N., Menichini, F., Noda, S., Minemoto, M., & Nakashima, K. (1998). Enantioselective high-performance liquid chromatography with fluorescence detection of methamphetamine and its metabolites in human urine. The Analyst, 123(11), 2333-7.
al-Dirbashi O, et al. Enantioselective High-performance Liquid Chromatography With Fluorescence Detection of Methamphetamine and Its Metabolites in Human Urine. Analyst. 1998;123(11):2333-7. PubMed PMID: 10396810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enantioselective high-performance liquid chromatography with fluorescence detection of methamphetamine and its metabolites in human urine. AU - al-Dirbashi,O, AU - Kuroda,N, AU - Menichini,F, AU - Noda,S, AU - Minemoto,M, AU - Nakashima,K, PY - 1999/7/9/pubmed PY - 1999/7/9/medline PY - 1999/7/9/entrez SP - 2333 EP - 7 JF - The Analyst JO - Analyst VL - 123 IS - 11 N2 - A simple, rapid and highly sensitive high-performance liquid chromatographic method with fluorescence detection for determining the enantiomers of methamphetamine and its major metabolites, amphetamine and p-hydroxymethamphetamine, in urine samples was developed. Using a newly developed reagent for amines, namely, 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride, six enantiomers were derivatized under mild conditions (i.e., 10 min at room temperature, pH 9.0) and separated isocratically on a cellulose tris(3,5-dimethylphenylcarbamate) coated silica gel column following a pre-separation on an ODS column within 42 min, and the effluent was monitored at 440 nm (lambda ex 330 nm). Calibration curves for these derivatives using spiked human urine were linear in the range 0.05-100 mumol dm-3 with correlation coefficients > or = 0.999. The detection limits at a signal-to-noise ratio of 3 were 2.8-8.8 fmol per 5 microliters injection. The relative standard deviations of within- (n = 6) and between-day (n = 5) variations were < or = 7.4%. The method was successfully applied to discriminate between (S)-(+)-methamphetamine and its corresponding metabolites found in abusers' urine and their antipodes in a sample taken from a Parkinsonian patient on selegiline (Deprenyl) therapy. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/10396810/Enantioselective_high_performance_liquid_chromatography_with_fluorescence_detection_of_methamphetamine_and_its_metabolites_in_human_urine_ L2 - https://doi.org/10.1039/a804720k DB - PRIME DP - Unbound Medicine ER -