Tags

Type your tag names separated by a space and hit enter

Cyclin D1 gene amplification and overexpression are present in ductal carcinoma in situ of the breast.
J Pathol. 1999 Feb; 187(3):279-84.JP

Abstract

Cyclin D1 (CCND1) amplification is found in 10-15 per cent of invasive breast carcinomas, but it is not well established whether this gene alteration also occurs in the precursor of invasive breast carcinoma, ductal carcinoma in situ (DCIS). By Southern blot analysis, cyclin D1 gene amplification was detected in 10 per cent (3/32) of DCIS cases. In addition, 15 cases of DCIS were analysed using bright field in situ hybridization (BRISH), of which 11 had already been analysed by Southern blotting. One additional case with gene amplification was found by BRISH. The use of BRISH for the detection of gene amplification is shown to be a novel and reliable in situ method on paraffin-embedded tissue sections. By immunohistochemistry, 147 cases of DCIS were analysed for the expression of cyclin D1. Cyclin D1 overexpression was found in 9 per cent of well-differentiated, 29 per cent of intermediately differentiated, and 19 per cent of poorly differentiated DCIS. No statistically significant association was found between cyclin D1 overexpression and the differentiation grade of DCIS, although 90 per cent of the cases that show overexpression are classified as intermediately and poorly differentiated. An association was found between cyclin D1 overexpression and oestrogen receptor positivity. Cyclin D1 overexpression was found in all four cases with cyclin D1 gene amplification, but was also found in 30 per cent (8/27) of cases without detectable gene amplification. It is concluded that cyclin D1 gene amplification is an early event in the development of breast carcinoma and occurs in poorly differentiated DCIS. Cyclin D1 protein overexpression is also present in tumours without cyclin D1 gene amplification and is seen predominantly in DCIS of intermediately and poorly differentiated histological type and oestrogen receptor positivity.

Authors+Show Affiliations

Department of Pathology, Leiden University Medical Centre, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10398079

Citation

Vos, C B., et al. "Cyclin D1 Gene Amplification and Overexpression Are Present in Ductal Carcinoma in Situ of the Breast." The Journal of Pathology, vol. 187, no. 3, 1999, pp. 279-84.
Vos CB, Ter Haar NT, Peterse JL, et al. Cyclin D1 gene amplification and overexpression are present in ductal carcinoma in situ of the breast. J Pathol. 1999;187(3):279-84.
Vos, C. B., Ter Haar, N. T., Peterse, J. L., Cornelisse, C. J., & van de Vijver, M. J. (1999). Cyclin D1 gene amplification and overexpression are present in ductal carcinoma in situ of the breast. The Journal of Pathology, 187(3), 279-84.
Vos CB, et al. Cyclin D1 Gene Amplification and Overexpression Are Present in Ductal Carcinoma in Situ of the Breast. J Pathol. 1999;187(3):279-84. PubMed PMID: 10398079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cyclin D1 gene amplification and overexpression are present in ductal carcinoma in situ of the breast. AU - Vos,C B, AU - Ter Haar,N T, AU - Peterse,J L, AU - Cornelisse,C J, AU - van de Vijver,M J, PY - 1999/7/9/pubmed PY - 1999/7/9/medline PY - 1999/7/9/entrez SP - 279 EP - 84 JF - The Journal of pathology JO - J Pathol VL - 187 IS - 3 N2 - Cyclin D1 (CCND1) amplification is found in 10-15 per cent of invasive breast carcinomas, but it is not well established whether this gene alteration also occurs in the precursor of invasive breast carcinoma, ductal carcinoma in situ (DCIS). By Southern blot analysis, cyclin D1 gene amplification was detected in 10 per cent (3/32) of DCIS cases. In addition, 15 cases of DCIS were analysed using bright field in situ hybridization (BRISH), of which 11 had already been analysed by Southern blotting. One additional case with gene amplification was found by BRISH. The use of BRISH for the detection of gene amplification is shown to be a novel and reliable in situ method on paraffin-embedded tissue sections. By immunohistochemistry, 147 cases of DCIS were analysed for the expression of cyclin D1. Cyclin D1 overexpression was found in 9 per cent of well-differentiated, 29 per cent of intermediately differentiated, and 19 per cent of poorly differentiated DCIS. No statistically significant association was found between cyclin D1 overexpression and the differentiation grade of DCIS, although 90 per cent of the cases that show overexpression are classified as intermediately and poorly differentiated. An association was found between cyclin D1 overexpression and oestrogen receptor positivity. Cyclin D1 overexpression was found in all four cases with cyclin D1 gene amplification, but was also found in 30 per cent (8/27) of cases without detectable gene amplification. It is concluded that cyclin D1 gene amplification is an early event in the development of breast carcinoma and occurs in poorly differentiated DCIS. Cyclin D1 protein overexpression is also present in tumours without cyclin D1 gene amplification and is seen predominantly in DCIS of intermediately and poorly differentiated histological type and oestrogen receptor positivity. SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/10398079/Cyclin_D1_gene_amplification_and_overexpression_are_present_in_ductal_carcinoma_in_situ_of_the_breast_ L2 - https://doi.org/10.1002/(SICI)1096-9896(199902)187:3<279::AID-PATH240>3.0.CO;2-C DB - PRIME DP - Unbound Medicine ER -