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Determination of the effect of pH and foetal haemoglobin fraction on haemoglobin oxygen saturation readings by the OSM3 hemoximeter.
Scand J Clin Lab Invest. 1999 May; 59(3):233-7.SJ

Abstract

The visible absorption spectrum of oxyhaemoglobin depends on its content of foetal haemoglobin and on the pH of the blood. These variables are known to affect oxygen saturation readings measured by the OSM3 Hemoximeter. As the hemoximeter estimates foetal haemoglobin content from the absorption spectrum of oxyhaemoglobin, this estimation is affected by pH. We quantified the effect of pH on oxyhaemoglobin reading in order to calculate a correction factor to adjust for pH. We manipulated a pool of fresh heparinized cord blood samples to produce a range of pH values from 6.99-7.53 in fully oxygenated blood with a carbon dioxide tension of 5.3 kPa. We measured oxygen saturation and percent foetal haemoglobin using an OSM3 Hemoximeter and pH with a linked blood gas analyser. The effect of pH on readings of saturation and fractional foetal haemoglobin was confirmed and a correction factor was calculated. The value of KHbF, the coefficient used in the OSM3 Hemoximeter to estimate the amount of fractional foetal haemoglobin present in a sample, was determined to be 18.3, which slightly but significantly differed (p = 0.04) from the 18.6 used by the OSM3 Hemoximeter. An equation was derived to correct the errors made by pH on the saturation measurement of oxygenated blood.

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Publisher Full Text

Authors+Show Affiliations

Mackay AR
Department of Pathology, IWK Grace Health Centre, Halifax, Nova Scotia, Canada.
Dooley KC
No affiliation info available
Whyte RK
No affiliation info available

MeSH

AdultBlood Gas AnalysisCarbon DioxideFemaleFetal BloodFetal HemoglobinHumansHydrogen-Ion ConcentrationOximetryOxyhemoglobinsPregnancyReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10400168

Citation

Mackay, A R., et al. "Determination of the Effect of pH and Foetal Haemoglobin Fraction On Haemoglobin Oxygen Saturation Readings By the OSM3 Hemoximeter." Scandinavian Journal of Clinical and Laboratory Investigation, vol. 59, no. 3, 1999, pp. 233-7.
Mackay AR, Dooley KC, Whyte RK. Determination of the effect of pH and foetal haemoglobin fraction on haemoglobin oxygen saturation readings by the OSM3 hemoximeter. Scand J Clin Lab Invest. 1999;59(3):233-7.
Mackay, A. R., Dooley, K. C., & Whyte, R. K. (1999). Determination of the effect of pH and foetal haemoglobin fraction on haemoglobin oxygen saturation readings by the OSM3 hemoximeter. Scandinavian Journal of Clinical and Laboratory Investigation, 59(3), 233-7.
Mackay AR, Dooley KC, Whyte RK. Determination of the Effect of pH and Foetal Haemoglobin Fraction On Haemoglobin Oxygen Saturation Readings By the OSM3 Hemoximeter. Scand J Clin Lab Invest. 1999;59(3):233-7. PubMed PMID: 10400168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of the effect of pH and foetal haemoglobin fraction on haemoglobin oxygen saturation readings by the OSM3 hemoximeter. AU - Mackay,A R, AU - Dooley,K C, AU - Whyte,R K, PY - 1999/7/10/pubmed PY - 1999/7/10/medline PY - 1999/7/10/entrez SP - 233 EP - 7 JF - Scandinavian journal of clinical and laboratory investigation JO - Scand J Clin Lab Invest VL - 59 IS - 3 N2 - The visible absorption spectrum of oxyhaemoglobin depends on its content of foetal haemoglobin and on the pH of the blood. These variables are known to affect oxygen saturation readings measured by the OSM3 Hemoximeter. As the hemoximeter estimates foetal haemoglobin content from the absorption spectrum of oxyhaemoglobin, this estimation is affected by pH. We quantified the effect of pH on oxyhaemoglobin reading in order to calculate a correction factor to adjust for pH. We manipulated a pool of fresh heparinized cord blood samples to produce a range of pH values from 6.99-7.53 in fully oxygenated blood with a carbon dioxide tension of 5.3 kPa. We measured oxygen saturation and percent foetal haemoglobin using an OSM3 Hemoximeter and pH with a linked blood gas analyser. The effect of pH on readings of saturation and fractional foetal haemoglobin was confirmed and a correction factor was calculated. The value of KHbF, the coefficient used in the OSM3 Hemoximeter to estimate the amount of fractional foetal haemoglobin present in a sample, was determined to be 18.3, which slightly but significantly differed (p = 0.04) from the 18.6 used by the OSM3 Hemoximeter. An equation was derived to correct the errors made by pH on the saturation measurement of oxygenated blood. SN - 0036-5513 UR - https://www.unboundmedicine.com/medline/citation/10400168/Determination_of_the_effect_of_pH_and_foetal_haemoglobin_fraction_on_haemoglobin_oxygen_saturation_readings_by_the_OSM3_hemoximeter_ L2 - https://www.tandfonline.com/doi/full/10.1080/00365519950185760 DB - PRIME DP - Unbound Medicine ER -