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hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing.
EMBO J. 1999 Jul 15; 18(14):4060-7.EJ

Abstract

Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are necessary for HIV-1 splicing inhibition. We demonstrate that heterogeneous nuclear ribonucleoproteins (hnRNPs) of the A and B group are trans-acting factors required for the function of the tat exon 2 ESS. Depletion of hnRNP A/B proteins from HeLa cell nuclear extract activates splicing of tat exon 2 pre-mRNA substrate. Splicing inhibition is restored by addition of recombinant hnRNP A/B proteins to the depleted extract. A high-affinity hnRNP A1-binding sequence can substitute functionally for the ESS in tat exon 2. These results demonstrate that hnRNP A/B proteins are required for repression of HIV-1 splicing.

Authors+Show Affiliations

Department of Biology and Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California Santa Cruz, Santa Cruz, CA 95064, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10406810

Citation

Caputi, M, et al. "HnRNP A/B Proteins Are Required for Inhibition of HIV-1 pre-mRNA Splicing." The EMBO Journal, vol. 18, no. 14, 1999, pp. 4060-7.
Caputi M, Mayeda A, Krainer AR, et al. HnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J. 1999;18(14):4060-7.
Caputi, M., Mayeda, A., Krainer, A. R., & Zahler, A. M. (1999). HnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. The EMBO Journal, 18(14), 4060-7.
Caputi M, et al. HnRNP A/B Proteins Are Required for Inhibition of HIV-1 pre-mRNA Splicing. EMBO J. 1999 Jul 15;18(14):4060-7. PubMed PMID: 10406810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. AU - Caputi,M, AU - Mayeda,A, AU - Krainer,A R, AU - Zahler,A M, PY - 1999/7/16/pubmed PY - 1999/7/16/medline PY - 1999/7/16/entrez SP - 4060 EP - 7 JF - The EMBO journal JO - EMBO J VL - 18 IS - 14 N2 - Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are necessary for HIV-1 splicing inhibition. We demonstrate that heterogeneous nuclear ribonucleoproteins (hnRNPs) of the A and B group are trans-acting factors required for the function of the tat exon 2 ESS. Depletion of hnRNP A/B proteins from HeLa cell nuclear extract activates splicing of tat exon 2 pre-mRNA substrate. Splicing inhibition is restored by addition of recombinant hnRNP A/B proteins to the depleted extract. A high-affinity hnRNP A1-binding sequence can substitute functionally for the ESS in tat exon 2. These results demonstrate that hnRNP A/B proteins are required for repression of HIV-1 splicing. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/10406810/hnRNP_A/B_proteins_are_required_for_inhibition_of_HIV_1_pre_mRNA_splicing_ L2 - https://doi.org/10.1093/emboj/18.14.4060 DB - PRIME DP - Unbound Medicine ER -