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Physical mapping of the CC-chemokine gene cluster on the human 17q11. 2 region.
Genomics. 1999 Jul 15; 59(2):213-23.G

Abstract

Chemokines are a family of small secreted proteins that are involved in the trafficking of leukocytes by acting on G-protein-coupled receptors. Specific chemokines are also implicated in the regulation of angiogenesis and mobilization of hematopoietic cell precursors. Chemokines are subdivided into four groups on the basis of the relative positions of their conserved cysteines. For the CC-chemokine group, in which the first two (of four) conserved cysteines are adjacent, 22 members have been described so far. In this work, we have analyzed the genomic organization of these genes. We first assigned the genes encoding CC-chemokines to chromosomal regions and organized their relative positioning by using two radiation hybrid panels. Fifteen CC-chemokine genes were shown to be clustered within the 17q11.2 region of the human genome. These genes appeared to be segregated into two subclusters separated by about 2. 25 Mb (9 cR). Contigs of bacterial artificial chromosomes (BAC) covering these two subclusters were subsequently isolated and the localizations of the CC-chemokine genes within these contigs determined. The relative positioning of the BAC clones was determined with the help of fluorescence hybridization on combed genomic DNA. The cluster organization of the various CC-chemokine genes in the genome was found to be grossly consistent with their structural similarities. This map of the CC-chemokine gene cluster should facilitate the determination of the full sequence of the chromosomal region.

Authors+Show Affiliations

IRIBHN, Service de Génétique Médicale, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Brussels, B-1070, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10409433

Citation

Maho, A, et al. "Physical Mapping of the CC-chemokine Gene Cluster On the Human 17q11. 2 Region." Genomics, vol. 59, no. 2, 1999, pp. 213-23.
Maho A, Carter A, Bensimon A, et al. Physical mapping of the CC-chemokine gene cluster on the human 17q11. 2 region. Genomics. 1999;59(2):213-23.
Maho, A., Carter, A., Bensimon, A., Vassart, G., & Parmentier, M. (1999). Physical mapping of the CC-chemokine gene cluster on the human 17q11. 2 region. Genomics, 59(2), 213-23.
Maho A, et al. Physical Mapping of the CC-chemokine Gene Cluster On the Human 17q11. 2 Region. Genomics. 1999 Jul 15;59(2):213-23. PubMed PMID: 10409433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physical mapping of the CC-chemokine gene cluster on the human 17q11. 2 region. AU - Maho,A, AU - Carter,A, AU - Bensimon,A, AU - Vassart,G, AU - Parmentier,M, PY - 1999/7/20/pubmed PY - 1999/7/20/medline PY - 1999/7/20/entrez SP - 213 EP - 23 JF - Genomics JO - Genomics VL - 59 IS - 2 N2 - Chemokines are a family of small secreted proteins that are involved in the trafficking of leukocytes by acting on G-protein-coupled receptors. Specific chemokines are also implicated in the regulation of angiogenesis and mobilization of hematopoietic cell precursors. Chemokines are subdivided into four groups on the basis of the relative positions of their conserved cysteines. For the CC-chemokine group, in which the first two (of four) conserved cysteines are adjacent, 22 members have been described so far. In this work, we have analyzed the genomic organization of these genes. We first assigned the genes encoding CC-chemokines to chromosomal regions and organized their relative positioning by using two radiation hybrid panels. Fifteen CC-chemokine genes were shown to be clustered within the 17q11.2 region of the human genome. These genes appeared to be segregated into two subclusters separated by about 2. 25 Mb (9 cR). Contigs of bacterial artificial chromosomes (BAC) covering these two subclusters were subsequently isolated and the localizations of the CC-chemokine genes within these contigs determined. The relative positioning of the BAC clones was determined with the help of fluorescence hybridization on combed genomic DNA. The cluster organization of the various CC-chemokine genes in the genome was found to be grossly consistent with their structural similarities. This map of the CC-chemokine gene cluster should facilitate the determination of the full sequence of the chromosomal region. SN - 0888-7543 UR - https://www.unboundmedicine.com/medline/citation/10409433/Physical_mapping_of_the_CC_chemokine_gene_cluster_on_the_human_17q11__2_region_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0888-7543(99)95850-0 DB - PRIME DP - Unbound Medicine ER -